| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001454 |
| 产品用途: | 3' AAVLINK plasmid for ZMYM2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ZMYM2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ZMYM2-FLAG 6676 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6676)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6676
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3377
/codon_start=1
/label=ZMYM2
/translation="V*LL*ISRNS*RASSVAWGNETFL*STLLTAFLLSTK*AQHDNSE
RT*KLTL*SGLSDISNQNDRFSTTPFSNT*QRDEEQSSSLQTFNNDKSYLL*TSHADQI
LSDR*YLEDRICSSAYPCACVYPSSYAHVQSEYSCSYYSSCSCASSCFSACSIGQQ*ED
SCSN*GAKKQGFFRCS*YRVAYNDGYDE*RRGENRDNQHQQCNY*NRYNWFRPFEEL*P
RDTVQHA*CTI*TRFGYRNRFSQSC*GA*YGK*IFITTCFWRRI*GTAQTSI*KKGSQE
KGCIRIPVS***F*QFRMQLSFQIYVWRKCMETLGQN*AT**RSSGIR*VKIF*ISKVK
RGSTLSHHS*A*LWVSSFCQ*DPTAKWRELCT*QHLLPLPWNTGVFVWK*SKRQHIY*S
WIPNI*ARIE*NTAKLATKHTSRWVNILSS*RRLSLEDKTTRITLSSSSSEYTVLL*H*
VFWPENSGTTLKTFLWHCV*ALEKKSFNDGKQSVSSIPSVFLVWNR**R*NYYWKKKT*
R**ASI*TN*KHSQSFQMSCENV*MLLV*KSTES*SEDGCFLFATRML*FYR*PCLVYV
YFTGPKHLGKYACTGSSSKRYL**RQL*TG*RHR"
CDS 3384..3449
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3465..3896
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3609..3614
/label=SDS
repeat_region 3939..4079
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3939..4068
/label=AAV2 ITR
rep_origin 4154..4609
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4891..4995
/gene="bla"
/label=AmpR promoter
CDS 4996..5856
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6027..6615
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt gactgttgta
1561 aatctcaagg aactcttaaa gagcgagttc agtggcgtgg ggaaatgaaa catttctgtg
1621 atcaacattg cttactgcgt ttctactgtc aacaaaatga gcccaacatg acaactcaga
1681 aaggacctga aaacttacat tatgatcagg gttgtcagac atctcgaacc aaaatgacag
1741 gttcagcacc acccccttct ccaacaccta acaaagagat gaagaacaaa gcagttcttt
1801 gcaaaccttt aacaatgaca aaagctactt actgtaaacc tcacatgcag accaaatctt
1861 gtcagacaga tgatacttgg aggacagaat atgttccagt gcctatccct gtgcctgtgt
1921 atatcccagt tcctatgcac atgtacagtc agaatattcc tgttcctact acagttcctg
1981 ttcctgtgcc agttcctgtt tttctgcctg ctccattgga cagcagtgag aagattcatg
2041 cagcaattga ggagctaaaa agcaaggttt cttcagatgc tcttgataca gagttgctta
2101 caatgacgga tatgatgagt gaagacgagg ggaaaacaga gacaaccaac atcaacagtg
2161 taattattga aacagatata attggttcag accttttgaa gaactctgac ccagagacac
2221 agtccagcat gcctgatgta ccatatgaac cagatttgga tatcgaaata gattttccca
2281 gagctgctga ggagcttgat atggaaaatg aatttttatt accacctgtt tttggcgaag
2341 aatatgagga acagcccaga cctcgatcta aaaaaaaggg agccaagaga aaggctgtat
2401 caggatacca gtctcatgat gatagttctg acaattcaga atgcagcttt cctttcaaat
2461 atacgtatgg cgtaaatgca tggaaacact gggtcaaaac taggcaactt gatgaagatc
2521 ttctggtatt agatgagtta aaatcttcta aatcagtaaa gttaaaagag gatctactct
2581 ctcacaccac agctgagctt aactatgggt tagctcattt tgtcaatgag atccgacggc
2641 caaatggaga gaattatgca cctgacagca tctattacct ttgccttgga atacaggagt
2701 atttgtgtgg aagtaatcga aaagacaaca tatttattga tcctggatac caaacatttg
2761 agcaagaatt gaataaaata ctgcgaagct ggcaaccaag catacttcca gatgggtcaa
2821 tattctctcg agttgaagaa gactatctct ggaggataaa acaactagga tcacactctc
2881 cagtagctct tctgaataca ctgttctact ttaacactaa gtattttggc ctgaaaacag
2941 tggaacaaca cttaagactt tcctttggca ctgtgtttag gcattggaaa aaaaatcctt
3001 taacgatgga aaacaaagcg tgtcttcgat accaagtgtc ttccttgtgt ggaacagata
3061 atgaagataa aattactact ggaaaaagaa aacatgaaga tgatgagcca gtatttgaac
3121 aaattgaaaa cacagccaat ccttccagat gtcctgtgaa aatgtttgaa tgctacttgt
3181 ctaaaagtcc acagaatctt aatcagagga tggatgtttt ttatttgcaa ccagaatgct
3241 ctagttctac agatagccct gtctggtata cgtctacttc actggaccga aacaccttgg
3301 aaaatatgct tgtacgggtt cttctagtaa aagatattta tgataaagac aattatgaac
3361 tggatgaaga cacagactgt acagactaca aagaccatga cggtgattat aaagatcatg
3421 acatcgacta caaggatgac gatgacaagt aagaattctg atgactcgag ataatcaacc
3481 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
3541 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
3601 cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc
3661 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
3721 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa
3781 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3841 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat
3901 ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt
3961 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
4021 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
4081 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
4141 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
4201 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
4261 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
4321 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
4381 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
4441 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
4501 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
4561 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
4621 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
4681 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
4741 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
4801 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
4861 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
4921 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
4981 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
5041 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
5101 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
5161 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
5221 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
5281 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
5341 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
5401 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
5461 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
5521 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
5581 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
5641 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
5701 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
5761 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
5821 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
5881 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
5941 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
6001 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
6061 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
6121 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
6181 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
6241 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
6301 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
6361 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
6421 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
6481 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
6541 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
6601 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
6661 ccttttgctc acatgt
//
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