| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001508 |
| 产品用途: | 3' AAVLINK plasmid for TOP2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TOP2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TOP2B-FLAG 6834 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6834)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6834
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3547
/codon_start=1
/label=TOP2B
/translation="VLEPMLNGTDKTPALISDYKEYHTDTTVKFVVKMTEEKLAQAEAA
GLHKVFKLQTTLTCNSMVLFDHMGCLKKYETVQDILKEFFDLRLSYYGLRKEWLVGMLG
AESTKLNNQARFILEKIQGKITIENRSKKDLIQMLVQRGYESDPVKAWKEAQEKAAEED
ETQNQHDDSSSDSGTPSGPDFNYILNMSLWSLTKEKVEELIKQRDAKGREVNDLKRKSP
SDLWKEDLAAFVEELDKVESQEREDVLAGMSGKAIKGKVGKPKVKKLQLEETMPSPYGR
RIIPEITAMKADASKKLLKKKKGDLDTAAVKVEFDEEFSGAPVEGAGEEALTPSVPINK
GPKPKREKKEPGTRVRKTPTSSGKPSAKKVKKRNPWSDDESKSESDLEETEPVVIPRDS
LLRRAAAERPKYTFDFSEEEDDDADDDDDDNNDLEELKVKASPITNDGEDEFVPSDGLD
KDEYTFSPGKSKATPEKSLHDKKSQDFGNLFSFPSYSQKSEDDSAKFDSNEEDSASVFS
PSFGLKQTDKVPSKTVAAKKGKPSSDTVPKPKRAPKQKKVVEAVNSDSDSEFGIPKKTT
TPKGKGRGAKKRKASGSENEGDYNPGRKTSKTTSKKPKKTSFDQDSDVDIFPSDFPTEP
PSLPRTGRARKEVKYFAESDEEEDDVDFAMFN"
CDS 3554..3619
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3623..4054
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3767..3772
/label=SDS
repeat_region 4097..4237
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4097..4226
/label=AAV2 ITR
rep_origin 4312..4767
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5049..5153
/gene="bla"
/label=AmpR promoter
CDS 5154..6014
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6185..6773
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttt tagaacctat
1561 gctaaatgga acagataaaa caccagcatt aatttctgat tataaagaat atcatactga
1621 cacaactgtg aaatttgtgg tgaaaatgac tgaagagaaa ctagcacaag cagaagctgc
1681 tggactgcat aaagttttta aacttcaaac tactcttact tgtaattcca tggtactttt
1741 tgatcatatg ggatgtctga agaaatatga aactgtgcaa gacattctga aagaattctt
1801 tgatttacga ttaagttatt acggtttacg taaggagtgg cttgtgggaa tgttgggagc
1861 agaatctaca aagctgaaca atcaagcccg tttcatttta gagaagatac aagggaaaat
1921 tactatagag aataggtcaa agaaagattt gattcaaatg ttagtccaga gaggttatga
1981 atctgaccca gtgaaagcct ggaaagaagc acaagaaaag gcagcagaag aggatgaaac
2041 acaaaaccag catgatgata gttcctccga ttcaggaact ccttcaggcc cagattttaa
2101 ttatatttta aatatgtctc tgtggtctct tactaaagaa aaagttgaag aactgattaa
2161 acagagagat gcaaaagggc gagaggtcaa tgatcttaaa agaaaatctc cttcagatct
2221 ttggaaagag gatttagcgg catttgttga agaactggat aaagtggaat ctcaagaacg
2281 agaagatgtt ctggctggaa tgtctggaaa agcaattaaa ggtaaagttg gcaaacctaa
2341 ggtgaagaaa ctccagttgg aagagacaat gccctcacct tatggcagaa gaataattcc
2401 tgaaattaca gctatgaagg cagatgccag caaaaagttg ctgaagaaga agaagggtga
2461 tcttgatact gcagcagtaa aagtggaatt tgatgaagaa ttcagtggag caccagtaga
2521 aggtgcagga gaagaggcat tgactccatc agttcctata aataaaggtc ccaaacctaa
2581 gagggagaag aaggagcctg gtaccagagt gagaaaaaca cctacatcat ctggtaaacc
2641 tagtgcaaag aaagtgaaga aacggaatcc ttggtcagat gatgaatcca agtcagaaag
2701 tgatttggaa gaaacagaac ctgtggttat tccaagagat tctttgctta ggagagcagc
2761 agccgaaaga cctaaataca catttgattt ctcagaagaa gaggatgatg atgctgatga
2821 tgatgatgat gacaataatg atttagagga attgaaagtt aaagcatctc ccataacaaa
2881 tgatggggaa gatgaatttg ttccttcaga tgggttagat aaagatgaat atacattttc
2941 accaggcaaa tcaaaagcca ctccagaaaa atctttgcat gacaaaaaaa gtcaggattt
3001 tggaaatctc ttctcatttc cttcatattc tcagaagtca gaagatgatt cagctaaatt
3061 tgacagtaat gaagaagatt ctgcttctgt tttttcacca tcatttggtc tgaaacagac
3121 agataaagtt ccaagtaaaa cggtagctgc taaaaaggga aaaccgtctt cagatacagt
3181 ccctaagccc aagagagccc caaaacagaa gaaagtagta gaggctgtaa actctgactc
3241 ggattcagaa tttggcattc caaagaagac tacaacacca aaaggtaaag gccgaggggc
3301 aaagaaaagg aaagcatctg gctctgaaaa tgaaggcgat tataaccctg gcaggaaaac
3361 atccaaaaca acaagcaaga aaccgaagaa gacatctttt gatcaggatt cagatgtgga
3421 catcttcccc tcagacttcc ctactgagcc accttctctg ccacgaaccg gtcgggctag
3481 gaaagaagta aaatattttg cagagtctga tgaagaagaa gatgatgttg attttgcaat
3541 gtttaattgt acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta
3601 caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa
3661 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
3721 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
3781 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
3841 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
3901 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
3961 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
4021 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
4081 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
4141 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
4201 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
4261 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4381 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4501 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4621 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
4681 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4741 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
4801 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
4861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
4921 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4981 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
5041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
5101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
5161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
5221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
5281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
5341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
5401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
5461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
5521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
6001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
6061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
6121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
6181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
6241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
6301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
6361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
6421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
6481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
