| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001532 |
| 产品用途: | 3' AAVLINK plasmid for TNRC6C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TNRC6C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TNRC6C-FLAG 6960 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6960)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6960
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3673
/codon_start=1
/label=KIF1A
/translation="DGGLVEEPTPSPFLPSPSLKLPLSHSALPSQALGGIASGLGMQNL
NSSRQIPSGNLGMFGNSGAAQARTMQQPPQPPVQPLNSSQPSLRAQVPQFLSPQVQAQL
LQFAAKNIGLNPALLTSPINPQHMTMLNQLYQLQLAYQRLQIQQQMLQAQRNVSGSMRQ
QEQQVARTITNLQQQIQQHQRQLAQALLVKQPPPPPPPPHLSLHPSAGKSAMDSFPSHP
QTPGLPDLQTKEQQSSPNTFAPYPLAGLNPNMNVNSMDMTGGLSVKDPSQSQSRLPQWT
HPNSMDNLPSAASPLEQNPSKHGAIPGGLSIGPPGKSSIDDSYGRYDLIQNSESPASPP
VAVPHSWSRAKSDSDKISNGSSINWPPEFHPGVPWKGLQNIDPENDPDVTPGSVPTGPT
INTTIQDVNRYLLKSGGSSPPSSQNATLPSSSAWPLSASGYSSSFSSIASAPSVAGKLS
DIKSTWSSGPTSHTQASLSHELWKVPRNSTAPTRPPPGLTNPKPSSTWGASPLGWTSSY
SSGSAWSTDTSGRTSSWLVLRNLTPQIDGSTLRTLCLQHGPLITFHLNLTQGNAVVRYS
SKEEAAKAQKSLHMCVLGNTTILAEFAGEEEVNRFLAQGQALPPTSSWQSSSASSQPRL
SAAGSSHGLVRSDAGHWNAPCLGGKGSSELLWGGVPQYSSSLWGPPSADDSRVIGSPTP
LTTLLPGDLLSGESL"
misc_feature 2776..2781
/label=SDS
misc_feature 3051..3056
/label=SDS
misc_feature 3279..3284
/label=SDS
misc_feature 3282..3287
/label=SDS
CDS 3680..3745
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3749..4180
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3893..3898
/label=SDS
repeat_region 4223..4363
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4223..4352
/label=AAV2 ITR
rep_origin 4438..4893
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5175..5279
/gene="bla"
/label=AmpR promoter
CDS 5280..6140
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6311..6899
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggatg gcggcctcgt
1561 ggaagagccc acgccttcac cgttcttgcc ttccccaagc ctgaagctcc ccctttcaca
1621 cagtgcactc cccagtcagg ccctgggtgg gattgcctcc gggctgggca tgcaaaactt
1681 gaattcttct agacagatac cgagtggcaa tctgggtatg tttggcaata gtggagcagc
1741 acaagccagg accatgcagc agccgccaca gccaccagtg cagcctctta actcttccca
1801 gcccagtctc cgtgctcaag tgcctcagtt tctatcccct caggttcaag cacagctttt
1861 gcagtttgca gcaaaaaaca ttggtctcaa ccctgcacta ttaacctcgc caattaatcc
1921 tcaacatatg acgatgttga accagctcta tcagctgcag ctggcatacc aacgtttaca
1981 aatccagcag cagatgttac aggcccagcg taatgtgtcc ggatccatga gacaacagga
2041 gcagcaagtt gcgcgcacaa tcactaatct gcagcagcag atccagcagc accagcgcca
2101 gctggcccag gccctgctcg tgaagcagcc accaccgcca ccgcccccgc cgcacctgtc
2161 tctgcacccc tctgcaggca aatcggccat ggacagcttc ccctcgcacc cacagactcc
2221 cggcctacct gacctgcaga ccaaagagca gcagtcttca cccaacacct ttgctcctta
2281 ccctctcgct ggactgaacc caaacatgaa tgtcaacagc atggacatga ccggtggctt
2341 gtcggtgaag gacccatccc agtcccagtc acgcctcccc cagtggacgc accccaactc
2401 catggataac ttgcccagtg ccgcttcccc cctggagcag aaccctagca agcatggtgc
2461 tatccctgga ggtctaagca ttgggcctcc aggtaagtcc tccattgatg actcctatgg
2521 ccggtacgat ttaatccaga acagtgagtc accagccagt cctcccgtag ctgttcccca
2581 tagctggtca cgtgccaaat ctgacagtga taaaatctca aatggctcta gcatcaactg
2641 gcccccagaa ttccatccgg gagttccatg gaaaggactg cagaatattg accctgagaa
2701 tgaccctgac gtcactcctg gcagtgtccc cactgggcct accatcaaca ccaccatcca
2761 ggatgtcaac cgctacctcc tcaagagtgg agggtcctcc ccgccatcat ctcagaatgc
2821 cacgctgcct tcttcgagtg cctggccact cagtgcctcc ggctacagta gctctttcag
2881 cagcattgca tccgcaccta gtgttgcagg taaactgtca gacatcaaat cgacgtggtc
2941 ctctggccct acctcccaca cgcaagcctc tctgtctcat gaactatgga aggtgcccag
3001 aaacagtact gcacccacga ggccacctcc agggttaacc aatcccaagc cctcctccac
3061 ctggggtgcc agccccctcg gttggaccag ctcctactcc tcgggttctg cctggagcac
3121 cgacacctca ggaagaacca gcagctggct cgttcttcga aacctcactc cccagattga
3181 tggttctaca ctgcggacat tgtgtttgca acatgggcct cttatcacat tccacctgaa
3241 tctgactcaa ggcaatgctg tggtccggta cagctccaag gaggaggctg ccaaggccca
3301 gaagtctctg cacatgtgcg tcctgggaaa cactaccatc ctggccgagt tcgctggtga
3361 agaagaagtg aatcgcttct tagcccaagg ccaggcgctg ccacccactt ccagctggca
3421 gtccagcagc gcgtccagcc agccgcggct cagcgcagcg ggcagctccc atggcctggt
3481 acgcagcgac gctggccact ggaacgcccc gtgcctgggt ggcaagggga gcagtgagct
3541 gctgtggggc ggggtgcccc agtactccag cagcctgtgg ggcccgccca gcgccgacga
3601 cagcagggtg ataggcagcc ccacgccgct aaccaccctg ctgcctgggg acctgctcag
3661 cggggagtcc ctgtgtacag actacaaaga ccatgacggt gattataaag atcatgacat
3721 cgactacaag gatgacgatg acaagtaact cgagataatc aacctctgga ttacaaaatt
3781 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
3841 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
3901 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
3961 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
4021 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
4081 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
4141 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
4201 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
4261 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc
4321 cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat
4381 tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa ccatagtacg
4441 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4501 cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4561 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4621 ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt agtgggccat
4681 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4741 tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt gatttataag
4801 ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4861 cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt acaatctgct
4921 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
4981 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
5041 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
5101 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
5161 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
5221 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
5281 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
5341 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
5401 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
5461 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
5521 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
5581 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
5641 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
5701 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
5761 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
5821 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
5881 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
5941 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
6001 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
6061 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
6121 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
6181 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
6241 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
6301 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
6361 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
6421 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
6481 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
6541 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
6601 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
6661 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
6721 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
6781 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
6841 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
6901 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
//
