| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001418 |
| 产品用途: | 3' AAVLINK plasmid for TCF20 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TCF20 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-TCF20-FLAG 7093 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7093)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7093
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3794
/codon_start=1
/label=TCF20
/translation="VWAAP*D*WTWTS*GYTVIQTW*CYAETSRPGGSFFSKPLNHEEA
CSFFYLSHSQ*ETVTRCKEQ*H*R*RSPPSLIKRRR**SIQFLCPSFSQSGYQVYP*ER
FLQGPSKSR**KLPCCYPHKPC*DQNTAPTERTGIEIGSYSSEDYIPKY*EERIFEQCG
GWGRHGYA**YTVFEEWSS*RWECCCSGC*HREEKR*GGFGPSQSSKPGVARRETSSKV
FRRVAWQRG*QSEDRDTCRNSYCRKGTPWCHDIHNLTEAW**PRETRWFPGWNSTFNLS
RLKECTSSGHIGP*GKPQG*REGERYSDDFTEARGFPSKGIFPIRKEEGETHW*CE*AK
ETAAATASTPSAPTDTRRFCRWRAKAKKTEAKEGEKEAWGPAEEAKNQTSSSHCGTPRT
*DQTKICHPATG*N*CQEQVFLPLHPCSK*V*TWSRLYNHQC*GRRTDQISEGQEGSEV
TDPST*QH*KQGAPGLVLYAAGTCCDRVFGYGAPGLLSVWQVGQLPEHG*PLWTFLSPR
LCSHSPEESTS*EGHRNAEQS*GTAQKCF*WLQDGH*GGGRAAAAAEGAEKPGRTPQV*
AAPPLGRLWWRPSVPVQGAPL*KSSH*GQQ*KDCFGLEALRAHHFRRWP*AGVTNP*TT
S*QQ*ILGP*GLYSLGQWNLPGLWQALWPAGSAGNSQRDEMFPLPGGRRHLGLLQQRLL
LPIPLPVCH*CRLFAT*GELLGEVP*AQASPSVPSPPLAEQDRERQPQHRAVGAG"
misc_feature 1639..1644
/label=SDS
misc_feature 1674..1679
/label=SDS
misc_feature 1763..1768
/label=SDS
misc_feature 2007..2012
/label=SDS
misc_feature 2088..2093
/label=SDS
misc_feature 2661..2666
/label=SDS
misc_feature 3126..3131
/label=SDS
misc_feature 3208..3213
/label=SDS
misc_feature 3211..3216
/label=SDS
misc_feature 3586..3591
/label=SDS
CDS 3801..3866
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3882..4313
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4026..4031
/label=SDS
repeat_region 4356..4496
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4356..4485
/label=AAV2 ITR
rep_origin 4571..5026
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5308..5412
/gene="bla"
/label=AmpR promoter
CDS 5413..6273
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6444..7032
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtat gggccgcccc
1561 atgagactga tggacatgga ctagctgagg ctacacagtc atccaaacct ggtagtgtta
1621 tgctgagact tccaggccag gaggatcatt cttctcaaaa ccccttaatc atgaggaggc
1681 gtgttcgttc ttttatctct cccattccca gtaagagaca gtcacaagat gtaaagaaca
1741 gtagcactga agataaaggt cgcctccttc actcatcaaa agaaggcgct gataaagcat
1801 tcaattccta tgcccatctt tctcacagtc aggatatcaa gtctatccct aagagagatt
1861 cctccaagga ccttccaagt ccagatagta gaaactgccc tgctgttacc ctcacaagcc
1921 ctgctaagac caaaatactg cccccacgga aaggacgggg attgaaattg gaagctatag
1981 ttcagaagat tacatcccca aatattagga ggagcgcatc ttcgaacagt gcggaggctg
2041 ggggagacac ggttacgctt gatgatatac tgtctttgaa gagtggtcct cctgaaggtg
2101 ggagtgttgc tgttcaggat gctgacatag agaagagaaa aggtgaggtg gcttcggacc
2161 tagtcagtcc agcaaaccag gagttgcacg tagagaaacc tcttccaagg tcttcagaag
2221 agtggcgtgg cagcgtggat gacaaagtga agacagagac acatgcagaa acagttactg
2281 ccggaaagga accccctggt gccatgacat ccacaacctc acagaagcct ggtagtaacc
2341 aagggagacc agatggttcc ctgggtggaa cagcaccttt aatctttcca gactcaaaga
2401 atgtacctcc agtgggcata ttggcccctg aggcaaaccc caaggctgaa gagaaggaga
2461 acgatacagt gacgatttca ccgaagcaag agggtttccc tccaaaggga tatttcccat
2521 caggaaagaa gaaggggaga cccattggta gtgtgaataa gcaaaagaaa cagcagcagc
2581 caccgcctcc accccctcag cccccacaga taccagaagg ttctgcagat ggagagccaa
2641 agccaaaaaa acagaggcaa aggagggaga gaaggaagcc tggggcccag ccgaggaagc
2701 gaaaaaccaa acaagcagtt cccattgtgg aaccccaaga acctgagatc aaactaaaat
2761 atgccaccca gccactggat aaaactgatg ccaagaacaa gtctttttac ccttacatcc
2821 atgtagtaaa taagtgtgaa cttggagccg tttgtacaat catcaatgct gaggaagaag
2881 aacagaccaa attagtgagg ggcaggaagg gtcagaggtc actgacccct ccacctagca
2941 gcactgaaag caaggcgctc ccggcctcgt cctttatgct gcagggacct gttgtgacag
3001 agtcttcggt tatggggcac ctggtttgct gtctgtgtgg caagtgggcc agttaccgga
3061 acatgggtga cctctttgga cctttttatc cccaagatta tgcagccact ctcccgaaga
3121 atccacctcc taagagggcc acagaaatgc agagcaaagt taaggtacgg cacaaaagtg
3181 cttctaatgg ctccaagacg gacactgagg aggaggaaga gcagcagcag cagcagaagg
3241 agcagagaag cctggccgca caccccaggt ttaagcggcg ccaccgctcg gaagactgtg
3301 gtggaggccc tcggtccctg tccagggggc tcccttgtaa aaaagcagcc actgagggca
3361 gcagtgaaaa gactgttttg gactcgaagc cctccgtgcc caccacttca gaaggtggcc
3421 ctgagctgga gttacaaatc cctgaactac ctcttgacag caatgaattt tgggtccatg
3481 agggttgtat tctctgggcc aatggaatct acctggtttg tggcaggctc tatggcctgc
3541 aggaagcgct ggaaatagcc agagagatga aatgttccca ctgccaggag gcaggcgcca
3601 ccttgggctg ctacaacaaa ggctgctcct tccgatacca ttacccgtgt gccattgatg
3661 cagattgttt gctacatgag gagaacttct cggtgaggtg ccctaagcac aagcctcccc
3721 ttccgtgccc tctccccccc ttgcagaaca agaccgcgaa aggcagcctc agcacagagc
3781 agtcggagcg ggggtgtaca gactacaaag accatgacgg tgattataaa gatcatgaca
3841 tcgactacaa ggatgacgat gacaagtaag aattctgatg actcgagata atcaacctct
3901 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
3961 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
4021 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
4081 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat
4141 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt
4201 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
4261 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg
4321 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
4381 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
4441 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
4501 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
4561 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
4621 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
4681 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
4741 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
4801 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
4861 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
4921 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
4981 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
5041 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
5101 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
5161 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
5221 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
5281 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
5341 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
5401 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
5461 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
5521 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
5581 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
5641 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
5701 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
5761 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
5821 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
5881 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
5941 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
6001 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
6061 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
6121 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
6181 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
6241 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
6301 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
6361 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
6421 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
6481 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
6541 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
6601 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
6661 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
6721 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
6781 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
6841 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
6901 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
6961 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
7021 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
7081 tttgctcaca tgt
//
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