| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001382 |
| 产品用途: | 3' AAVLINK plasmid for SYNGAP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SYNGAP1 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SYNGAP1-FLAG 7050 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7050)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7050
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3763
/codon_start=1
/label=SYNGAP1
/translation="VVNSHCVFPRELKEVFASWRLRCAERGREDIADRLISASLFLRFL
CPAIMSPSLFGLMQEYPDEQTSRTLTLIAKVIQNLANFSKFTSKEDFLGFMNEFLELEW
GSMQQFLYEISNLDTLTNSSSFEGYIDLGRELSTLHALLWEVLPQLSKEALLKLGPLPR
LLNDISTALRNPNIQRQPSRQSERPRPQPVVLRGPSAEMQGYMMRDLNSSMDMARLPSP
TKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKSVSMLDLQG
DGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLS
QMGVTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPGGHGGGGGHGPPSSHHHHHHHHHH
RGGEPPGDTFAPFHGYSKSEDLSSGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQ
DNLQHMLSPPQITIGPQRPAPSGPGGGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKP
RPSSGNLLQSPEPSYGPARPRQQSLSKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPST
LNPTMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLDREYEEEIHS
LKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQAEKDSQIKSII
GRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV"
misc_feature 1584..1589
/label=SDS
misc_feature 1812..1817
/label=SDS
misc_feature 2008..2013
/label=SDS
CDS 2714..2743
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
misc_feature 3177..3182
/label=SDS
misc_feature 3435..3440
/label=SDS
misc_feature 3648..3653
/label=SDS
CDS 3770..3835
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3839..4270
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3983..3988
/label=SDS
repeat_region 4313..4453
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4313..4442
/label=AAV2 ITR
rep_origin 4528..4983
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5265..5369
/gene="bla"
/label=AmpR promoter
CDS 5370..6230
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6401..6989
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgg tcaactccca
1561 ctgcgtgttc ccgagggagc tgaaggaggt gtttgcttca tggcggctgc gctgcgcaga
1621 gcgaggccgg gaggacatcg cagacaggct tatcagcgcc tcactcttcc tgcgcttcct
1681 ctgcccagcg attatgtcgc ccagtctctt tgggcttatg caggagtacc cagatgagca
1741 gacctcacga accctcaccc tcattgccaa ggtcatccag aacctggcca acttttccaa
1801 gtttacctca aaggaggact ttctgggctt catgaatgag tttctggagc tggaatgggg
1861 ttccatgcag cagtttttgt atgagatctc caatctggac acgctaacca acagcagtag
1921 ctttgagggt tacatcgact tgggccgaga gctctccaca ctgcatgccc tactctggga
1981 ggtgctgccc cagctcagca aggaagccct cctgaagctg ggtccactgc cccggctcct
2041 caacgacatc agcacagctc tgaggaaccc caacatccaa aggcagccaa gccgccagag
2101 tgagcggccc cggcctcagc ctgtggtact gcgggggcca tcggctgaga tgcagggcta
2161 catgatgcgg gacctcaaca gctctatgga catggctcgc ctcccctccc caaccaagga
2221 aaagccaccc ccaccaccgc ctggtggtgg taaagacctg ttctatgtaa gccgtccacc
2281 cctggcccgt tcctcaccag catactgcac gagcagctcg gacatcacag agccagagca
2341 gaagatgctg agtgtcaaca agagtgtgtc catgctggac ttacagggtg atgggcctgg
2401 tggccgcctc aacagcagca gtgtttcgaa cctggcggcc gtaggggacc tgctgcactc
2461 aagccaggcc tcgctgacag cagccttggg gctacggcct gcgcctgccg gacgcctctc
2521 ccaggggagt ggctcatcca tcacggcggc tggcatgcgc ctcagccaga tgggtgtcac
2581 cacagacggt gtccctgccc agcaactgcg aatccccctc tccttccaga accctctctt
2641 ccacatggct gctgatgggc caggtccccc aggcggccat ggagggggcg gtggccatgg
2701 cccaccttcc tcccatcacc accaccacca ccatcaccac caccgaggtg gagagccccc
2761 tggggacacc tttgccccat tccatggcta tagcaagagt gaggacctct cttccggggt
2821 ccccaagccc cctgctgcct ccatccttca tagccacagc tacagtgatg agtttggacc
2881 ctctggcact gacttcaccc gtcggcagct ttcactccag gacaacctgc agcacatgct
2941 gtcccctccc cagatcacca ttggtcccca gaggccagcc ccctcagggc ctggaggtgg
3001 gagcggtggg ggcagcggtg ggggtggcgg gggccagccg cctccattgc agaggggcaa
3061 gtctcagcag ttgacagtca gcgcagccca gaaaccccgg ccatccagcg ggaatctatt
3121 gcagtcccca gagccaagtt atggccccgc ccgtccacgg caacagagcc tcagcaagga
3181 gggcagcatt gggggcagcg ggggcagcgg tggcggaggg ggtggggggc tgaagccctc
3241 catcaccaag cagcattctc agacaccatc cacattgaac cccacaatgc cagcctctga
3301 gcggacagtg gcctgggtct ccaacatgcc tcacctgtcg gctgacatcg agagtgccca
3361 catcgagcgg gaagagtaca agctcaagga gtactcaaaa tcgatggatg agagccggct
3421 ggatagggag tacgaggagg agattcactc actgaaagag cggctgcaca tgtccaaccg
3481 gaagctggaa gagtatgagc ggaggctgct gtcccaggaa gaacaaacca gcaaaatcct
3541 gatgcagtat caggcccgac tggagcagag tgagaagagg ctaaggcagc agcaggcaga
3601 gaaggattcc cagatcaaga gcatcattgg caggctgatg ctggtggagg aggagctgcg
3661 ccgggaccac cccgccatgg ctgagccgct gccagaaccc aagaagaggc tgctcgacgc
3721 tcagagaggc agcttccccc cttgggtcca acaaacccgc gtgtgtacag actacaaaga
3781 ccatgacggt gattacaaag atcatgacat cgactacaag gatgacgatg acaagtaact
3841 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
3961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
4021 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
4081 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
4141 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
4201 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
4261 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
4321 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4381 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4441 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4501 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4621 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4681 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4741 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4801 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4861 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4921 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
4981 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
5041 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
5101 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
5161 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
5221 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
5281 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
5341 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5401 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5461 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5521 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5581 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5641 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5701 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5761 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5821 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5881 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
5941 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
6001 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
6061 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
6121 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
6181 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
6241 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
6301 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6361 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6421 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6481 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6541 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6601 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6661 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6721 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6781 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6841 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6901 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6961 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
7021 tggccttttg ctggcctttt gctcacatgt
//
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