| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001394 |
| 产品用途: | 3' AAVLINK plasmid for SMARCA4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SMARCA4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG 6828 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6828)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6828
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3541
/codon_start=1
/label=SMARCA4
/translation="GVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESF
SEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAY
RGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSD
WNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQ
KSSSHERRAFLQAILEHEEQDESRHCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDE
VPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEV
ERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKKITGKDIHDTASSVARGLQFQRG
LQFCTRASKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQ
KKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYE
LIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFT
SVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPS
RGSRAKPVVSDDDSEEEQEEDRSGSGSEED"
misc_feature 2376..2381
/label=SDS
misc_feature 2433..2438
/label=SDS
misc_feature 2481..2486
/label=SDS
misc_feature 2520..2525
/label=SDS
misc_feature 2583..2588
/label=SDS
misc_feature 2586..2591
/label=SDS
misc_feature 2589..2594
/label=SDS
misc_feature 2628..2633
/label=SDS
misc_feature 2778..2783
/label=SDS
misc_feature 2805..2810
/label=SDS
misc_feature 3297..3302
/label=SDS
misc_feature 3327..3332
/label=SDS
misc_feature 3330..3335
/label=SDS
misc_feature 3345..3350
/label=SDS
misc_feature 3495..3500
/label=SDS
misc_feature 3507..3512
/label=SDS
CDS 3548..3613
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3617..4048
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3761..3766
/label=SDS
repeat_region 4091..4231
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4091..4220
/label=AAV2 ITR
rep_origin 4306..4761
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5043..5147
/gene="bla"
/label=AmpR promoter
CDS 5148..6008
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6179..6767
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggcg tgctgctgac
1561 tgatggctcc gagaaggaca agaagggcaa aggcggcacc aagaccctga tgaacaccat
1621 catgcagctg cggaagatct gcaaccaccc ctacatgttc cagcacatcg aggagtcctt
1681 ttccgagcac ttggggttca ctggcggcat tgtccaaggg ctggacctgt accgagcctc
1741 gggtaaattt gagcttcttg atagaattct tcccaaactc cgagcaacca accacaaagt
1801 gctgctgttc tgccaaatga cctccctcat gaccatcatg gaagattact ttgcgtatcg
1861 cggctttaaa tacctcaggc ttgatggaac cacgaaggcg gaggaccggg gcatgctgct
1921 gaaaaccttc aacgagcccg gctctgagta cttcatcttc ctgctcagca cccgggctgg
1981 ggggctcggc ctgaacctcc agtcggcaga cactgtgatc atttttgaca gcgactggaa
2041 tcctcaccag gacctgcaag cgcaggaccg agcccaccgc atcgggcagc agaacgaggt
2101 gcgtgtgctc cgcctctgca ccgtcaacag cgtggaggag aagatcctag ctgcagccaa
2161 gtacaagctc aacgtggacc agaaggtgat ccaggccggc atgttcgacc agaagtcctc
2221 cagccatgag cggcgcgcct tcctgcaggc catcctggag cacgaggagc aggatgagag
2281 cagacactgc agcacgggca gcggcagtgc cagcttcgcc cacactgccc ctccgccagc
2341 gggcgtcaac cccgacttgg aggagccacc tctaaaggag gaagacgagg tgcccgacga
2401 cgagaccgtc aaccagatga tcgcccggca cgaggaggag tttgatctgt tcatgcgcat
2461 ggacctggac cgcaggcgcg aggaggcccg caaccccaag cggaagccgc gcctcatgga
2521 ggaggacgag ctcccctcgt ggatcatcaa ggacgacgcg gaggtggagc ggctgacctg
2581 tgaggaggag gaggagaaga tgttcggccg tggctcccgc caccgcaagg aggtggacta
2641 cagcgactca ctgacggaga agcagtggct caagaaaatt acaggaaaag atatccatga
2701 cacagccagc agtgtggcac gtgggctaca attccagcgt ggccttcagt tctgcacacg
2761 tgcgtcaaag gccatcgagg agggcacgct ggaggagatc gaagaggagg tccggcagaa
2821 gaaatcatca cggaagcgca agcgagacag cgacgccggc tcctccaccc cgaccaccag
2881 cacccgcagc cgcgacaagg acgacgagag caagaagcag aagaagcgcg ggcggccgcc
2941 tgccgagaaa ctctccccta acccacccaa cctcaccaag aagatgaaga agattgtgga
3001 tgccgtgatc aagtacaagg acagcagcag tggacgtcag ctcagcgagg tcttcatcca
3061 gctgccctcg cgaaaggagc tgcccgagta ctacgagctc atccgcaagc ccgtggactt
3121 caagaagata aaggagcgca ttcgcaacca caagtaccgc agcctcaacg acctagagaa
3181 ggacgtcatg ctcctgtgcc agaacgcaca gaccttcaac ctggagggct ccctgatcta
3241 tgaagactcc atcgtcttgc agtcggtctt caccagcgtg cggcagaaaa tcgagaagga
3301 ggatgacagt gaaggcgagg agagtgagga ggaggaagag ggcgaggagg aaggctccga
3361 atccgaatct cggtccgtca aagtgaagat caagcttggc cggaaggaga aggcacagga
3421 ccggctgaag ggcggccggc ggcggccgag ccgagggtcc cgagccaagc cggtcgtgag
3481 tgacgatgac agtgaggagg aacaagagga ggaccgctca ggaagtggca gcgaagaaga
3541 ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg actacaagga
3601 tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg tgaaagattg
3661 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
3721 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
3781 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
3841 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct
3901 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac
3961 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
4021 tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc
4081 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
4141 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
4201 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
4261 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
4321 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4381 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4441 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4501 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
4561 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
4621 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
4681 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4741 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
4801 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
4861 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
4921 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
4981 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
5041 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
5101 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
5161 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
5221 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
5281 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
5341 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
5401 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
5461 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
5521 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
5581 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
5641 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5701 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5761 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5821 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5881 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5941 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
6001 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
6061 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
6121 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
6181 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
6241 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
6301 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
6361 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
6421 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
6481 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
6541 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
6601 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6661 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6721 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6781 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA4-FLAG质粒载体应用举例
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