| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001397 |
| 产品用途: | 3' AAVLINK plasmid for SETD1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD1A |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG 6658 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6658)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6658
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3371
/codon_start=1
/label=SETD1A
/translation="GCRLPSHTPARTGGVSSKACRPHGGVTPQCASASPRTTCWAPGPC
PTPR*ASLFSHPPPAPTQETPENCLLLCHRGGASPGAPSSHTAAGQVSRPSLPQGSPGR
GADHPQPAPGPRISGQELARGGVPRRPEPGWRPRPPHRGRGG*ARDRGGPGGPGRPGPD
PCPARAACPACC*RLRGHRDIGRGRAP*APAQPHPPGAQLCPGRQAHAPCASPAAPGAS
ARTRRPLQFPS**GPGGPRGGGG*GGGAQAAATAAAAGGGRRGGGGRGGGRGGGVL*QQ
QQQRWGGRPPEAQPPLPRPAPPPSAPTPAATAPRLRATQ*V*TDDHPV*HLELGPGLRG
HELPAAYVRAAAAADKRG*LAQRHSLGPSHNHQPDHPKTQAAAPGWAPGAPDRLSPQRR
LLPHQQEGEGQVPGRVPSLGPAAGGRGHSGDEPRAVRAPVRAAAAAERHRYLRHHGQ*P
AETQPAQVPEEEAPIWPEPDPRVGSVCHGTHCC*RDGHRIRGSEHPSDGGRHAGEALRA
GGHWQQLPVPGGPRHHHRCHQVWQPGQIHQPLLHA*LLRQGHHHRVPEEDRDLLQAAHW
RGRGDHLRLQVPTGRQQDPVSVWHRELPGLPK"
misc_feature 1582..1587
/label=SDS
misc_feature 1718..1723
/label=SDS
misc_feature 1918..1923
/label=SDS
misc_feature 1931..1936
/label=SDS
misc_feature 1978..1983
/label=SDS
misc_feature 2138..2143
/label=SDS
misc_feature 2326..2331
/label=SDS
misc_feature 2338..2343
/label=SDS
misc_feature 2365..2370
/label=SDS
misc_feature 2368..2373
/label=SDS
misc_feature 3097..3102
/label=SDS
CDS 3378..3443
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3447..3878
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3591..3596
/label=SDS
repeat_region 3921..4061
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3921..4050
/label=AAV2 ITR
rep_origin 4136..4591
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4873..4977
/gene="bla"
/label=AmpR promoter
CDS 4978..5838
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6009..6597
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggtt gcaggctccc
1561 cagtcacacc cctgcccgaa caggaggcgt ctccagcaag gcctgcaggc cccacggagg
1621 agtcaccccc cagtgcgcct ctgcgtcccc cagaaccacc tgctgggccc ccggcccctg
1681 ccccacgccc cgatgagcgt ccctcttctc ccatccccct cctgccccca cccaagaaac
1741 gccggaaaac tgtctccttc tctgccatcg aggtggtgcc agccccggag ccccctccag
1801 ccacaccgcc gcaggccaag tttcccggcc cagcctcccg caaggctccc cggggcgtgg
1861 agcggaccat ccgcaacctg cccctggacc acgcatctct ggtcaagagt tggcccgagg
1921 aggtgtcccg aggaggccgg agccgggctg gaggccgagg ccgcctcacc gaggaagagg
1981 aggctgagcc agggacagag gtggacctgg cggtcctggc cgacctggcc ctgacccctg
2041 cccggcgcgg gctgcctgcc ctgcctgctg ttgaagactc agaggccaca gagacatcgg
2101 acgaggccga gcgccctagg cccctgctca gccacatcct cctggagcac aactatgccc
2161 tggccgtcaa gcccacgccc cctgcgccag ccctgcggcc cccggagcca gtgcccgcac
2221 ccgccgccct cttcagttcc ccagctgatg aggtcctgga ggcccccgag gtggtggtgg
2281 ctgaggcgga ggagcccaag ccgcagcaac tgcagcagca gcgggaggag ggcgaagagg
2341 agggggagga agagggggag gaagaggagg aggagtcctc tgacagcagc agcagcagcg
2401 atggggaggg cgccctccgg aggcgcagcc tccgctccca cgcccggcgc cgccgccctc
2461 cgcccccacc cccgccgcca ccgccccgcg cctacgagcc acgcagtgag tttgaacaga
2521 tgaccatcct gtatgacatt tggaactcgg gcctggactc agaggacatg agttacctgc
2581 ggcttacgta cgagcggctg ctgcagcaga caagcggggc tgactggctc aacgacactc
2641 actgggtcca tcacacaatc accaacctga ccaccccaaa acgcaagcgg cggccccagg
2701 atgggccccg ggagcaccag acaggctcag cccgcagcga aggctactac cccatcagca
2761 agaaggagaa ggacaagtac ctggacgtgt gcccagtctc ggcccggcag ctggagggcg
2821 tggacactca ggggacgaac cgcgtgctgt ccgagcgccg gtccgagcag cggcggctgc
2881 tgagcgccat cggtacctcc gccatcatgg acagtgacct gctgaaactc aaccagctca
2941 agttccggaa gaagaagctc cgatttggcc ggagccggat ccacgagtgg ggtctgtttg
3001 ccatggaacc cattgctgct gacgagatgg tcatcgaata cgtgggtcag aacatccgtc
3061 agatggtggc cgacatgcgg gagaagcgct acgtgcagga gggcattggc agcagctacc
3121 tgttccgggt ggaccacgac accatcatcg atgccaccaa gtgtggcaac ctggccagat
3181 tcatcaacca ctgctgcacg cctaactgct acgccaaggt catcaccatc gagtcccaga
3241 agaagatcgt gatctactcc aagcagccca ttggcgtgga cgaggagatc acctacgact
3301 acaagttccc actggaagac aacaagatcc cgtgtctgtg tggcacagag agctgccggg
3361 gctccctaaa ctgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3421 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
3481 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3541 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
3601 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
3661 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
3721 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
3781 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
3841 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
3901 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
3961 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4021 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4081 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4141 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4201 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4261 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4321 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4381 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4441 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4501 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4561 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
4621 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
4681 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
4741 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
4801 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
4861 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4921 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
4981 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5041 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5101 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5161 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5221 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5281 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5341 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5401 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5461 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5521 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5581 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
5641 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5701 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5761 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5821 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5881 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
5941 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6001 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6061 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6121 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6181 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6241 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6301 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6361 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6421 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6481 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6541 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6601 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SETD1A-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG
- 下一篇：没有了
