| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001457 |
| 产品用途: | 3' AAVLINK plasmid for ROBO2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ROBO2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ROBO2-FLAG 6821 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6821)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6821
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3534
/codon_start=1
/label=ROBO2
/translation="VFNTG*RLQLVPVQGLE*RVSHSQ**SGDAMKLSLLKTITA*LSK
SLMW*SNQPL*LVLVVPAG*F*WVLAYGCIGEERRGRDSVIMLLRFKEEMED**AMEAV
QVFSMLVIPAIHGLLILGQPRACQ*IIATVAQMRLEILAVEMCCHQFQAKGIKQQRCSQ
MEPFIVALTSLPKPVTTVPAK*HRLPHMPRHRSCIPTAYMNWLSICLIHNGKAQFSKKQ
I*WDLVILYLIRTKVTMVGKVEKRRKIKTLLNHRKTMDPLGPMSLYLPPQSSPFLARSW
NTMQWNNKKMAMTVIAGAHHCQYKLTYTKVWKMNWKKMMIGSQHLLFEAWLLLLLSPLD
SSPLQLLLHPHGKRCNPCCRLTWMS*QEPISLI*QNKHGTFKAIINLHSLQFHR*VMCL
EP*FLIWKRMLQMMMPTTKRKL*KSPGP*EHWTRLLDPAWTI*TAL*QEKPLPPLKDLD
LPAHFLLTVTPVQP*VKVRGLGPLKNTREGGWTNNQHCLIEGKE*QMIFHHHQIPRQVR
V*GSK*ARASRLVTWKTQQREKEAL*RDNMHPA*KTQRAHWIVQLEPP*SGSDKPRNG*
APQNDKKIYGKPHTNKVSDQRRPWCPIASPVSHLQVATAHQEQLLLRDPLDLGKPRC*E
QATSAMPATFLT*DIWAPTVKDSLQVN"
misc_feature 2521..2526
/label=SDS
misc_feature 2900..2905
/label=SDS
misc_feature 3335..3340
/label=SDS
CDS 3541..3606
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3610..4041
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3754..3759
/label=SDS
repeat_region 4084..4224
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4084..4213
/label=AAV2 ITR
rep_origin 4299..4754
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5036..5140
/gene="bla"
/label=AmpR promoter
CDS 5141..6001
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6172..6760
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtat tcaataccgg
1561 gtagaggttg cagctagtac cagtgcaggg gttggagtaa agagtgagcc acagccaata
1621 ataatcggga gacgcaatga agttgtcatt actgaaaaca ataacagcat aactgagcaa
1681 atcactgatg tggtgaagca accagccttt atagctggta ttggtggtgc ctgctgggta
1741 attctgatgg gttttagcat atggttgtat tggcgaagaa agaagaggaa gggactcagt
1801 aattatgctg ttacgtttca aagaggagat ggaggactaa tgagcaatgg aagccgtcca
1861 ggtcttctca atgctggtga tcccagctat ccatggcttg ctgattcttg gccagccacg
1921 agcttgccag taaataatag caacagtggc ccaaatgaga ttggaaattt tggccgtgga
1981 gatgtgctgc caccagttcc aggccaaggg gataaaacag caacgatgct ctcagatgga
2041 gccatttata gtagcattga cttcactacc aaaaccagtt acaacagttc cagccaaata
2101 acacaggcta ccccatatgc cacgacacag atcttgcatt ccaacagcat acatgaattg
2161 gctgtcgatc tgcctgatcc acaatggaaa agctcaattc agcaaaaaac agatctgatg
2221 ggatttggtt attctctacc tgatcagaac aaaggtaaca atggtgggaa aggtggaaaa
2281 aagaagaaaa ataaaaactc ttctaaacca cagaaaaaca atggatccac ttgggccaat
2341 gtccctctac ctcccccccc agtccagccc cttcctggca cggagctgga acactatgca
2401 gtggaacaac aagaaaatgg ctatgacagt gatagctggt gcccaccatt gccagtacaa
2461 acttacttac accaaggtct ggaagatgaa ctggaagaag atgatgatag ggtcccaaca
2521 cctcctgttc gaggcgtggc ttcttctcct gctatctcct ttggacagca gtccactgca
2581 actcttactc catccccacg ggaagagatg caacccatgc tgcaggctca cctggatgag
2641 ttgacaagag cctatcagtt tgatatagca aaacaaacat ggcacattca aagcaataat
2701 caacctccac agcctccagt tccaccgtta ggttatgtgt ctggagcctt gatttctgat
2761 ttggaaacgg atgttgcaga tgatgatgcc gacgacgaag aggaagcttt agaaatcccc
2821 aggcccctga gagcactgga ccagactcct ggatccagca tggacaatct agacagctct
2881 gtgacaggaa aagcctttac ctcctctcaa agacctcgac ctaccagccc attttctact
2941 gacagtaaca ccagtgcagc cctgagtcaa agtcagaggc ctcggcccac taaaaaacac
3001 aagggagggc ggatggacca acaaccagca ttgcctcatc gaagggaagg aatgacagat
3061 gatcttccac caccaccaga tcccccgcca ggtcagggtt taaggcagca aataggcccg
3121 agccagcagg ctggtaacgt ggaaaactca gcagagagaa aaggaagctc tctagagaga
3181 caacatgcat ccagcttaga agacacaaag agctcattgg attgtccagc tagaacctcc
3241 ctagagtggc agcgacaaac ccaggaatgg ataagctcca cagaacgaca agaagatata
3301 cggaaagccc cacacaaaca aggtgtcgga tcagaggagg ccttggtgcc ctatagcaag
3361 cccagtttcc catctccagg tggccacagc tcatcaggaa cagcttcttc taagggatcc
3421 actggaccta ggaaaaccga ggtgttgaga gcaggccacc agcgcaatgc cagcgacctt
3481 cttgacatag gatatatggg ctccaacagt caaggacagt ttacaggtga attatgtaca
3541 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
3601 gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga ttgactggta
3661 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
3721 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttagttc
3781 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
3841 tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt gctttatttg
3901 taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
3961 agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg
4021 gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg accgagcggc
4081 cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
4141 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
4201 agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt acgcatctgt
4261 gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta gcggcgcatt
4321 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccttagc
4381 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
4441 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
4501 caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
4561 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
4621 aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc cgatttcggt
4681 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
4741 aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg catagttaag
4801 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
4861 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
4921 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
4981 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
5041 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
5101 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
5161 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
5221 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
5281 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
5341 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
5401 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
5461 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
5521 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
5581 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
5641 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
5701 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
5761 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
5821 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
5881 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
5941 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
6001 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
6061 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
6121 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
6181 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
6241 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
6301 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
6361 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
6421 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
6481 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
6541 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
6601 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
6661 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
6721 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
6781 tttacggttc ctggcctttt gctggccttt tgctcacatg t
//
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