pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RIMS2-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RIMS2-FLAG               6486 bp DNA     circular SYN 18-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 21, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3199
                     /codon_start=1
                     /label=RIMS2
                     /translation="GLRGTRTMTGHYNTISRMDRHRVMDDHYSPDRDRDCEAADRQPYH
                     RSRSTEQRPLLERTTTRSRSTERPDTNLMRSMPSLMTGRSAPPSPALSRSHPRTGSVQT
                     SPSSTPVAGRRGRQLPQLPPKGTLDRKELDSTRRRYAGTMDIEERNRQMKINKYKQVAG
                     SDPRLEQDYHSKYRSGWDPHRGADNVSTKSSDSDVSDISAVSRTSSASRFSSTSYMSVQ
                     SERPGGNKKISVFTSKMQSRQMGISGKNMTKSTSISGDMCSLEKNDGSQSDTAVGTLGT
                     SGKKRRSSLGAKMVAIVGLSRKSRSASQLSQTEAGGKKLRSTVQRSTETGLAVEMRNWM
                     TRQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMG
                     DIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARKT
                     LEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWFKL
                     FPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS"
     misc_feature    1805..1810
                     /label=SDS
     misc_feature    2224..2229
                     /label=SDS
     misc_feature    2491..2496
                     /label=SDS
     CDS             3206..3271
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3275..3706
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3419..3424
                     /label=SDS
     repeat_region   3749..3889
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3749..3878
                     /label=AAV2 ITR
     rep_origin      3964..4419
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4701..4805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4806..5666
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5837..6425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc ttcgagggac
     1561 ccgcactatg accggacatt ataatacaat tagccgaatg gacagacatc gtgtcatgga
     1621 tgaccattat tctccagata gagacaggga ttgtgaagca gcagatagac agccatatca
     1681 cagatccaga tcaacagaac aacggcctct ccttgagcgg accaccaccc gctccagatc
     1741 cactgaacgt cctgatacaa acctcatgag gtcgatgcct tcattaatga ctggaagatc
     1801 tgcccctcct tcacctgcct tatcgaggtc tcatcctcgt actgggtctg tccagacaag
     1861 cccatcaagt actccagtcg caggacgaag gggccgacag cttccacagc ttccaccaaa
     1921 gggaacgttg gatagaaaag aactggactc tacaaggaga agatatgcag gtactatgga
     1981 tatagaggag agaaatcgcc aaatgaaaat taacaaatac aaacaggtag ccggatcaga
     2041 tcccagactg gaacaagatt accattcgaa gtatcgatca ggatgggatc ctcatagagg
     2101 ggcagataat gtttctacta aatcttcgga cagtgatgta agtgatatat ctgcggtttc
     2161 aaggactagt agtgcttctc gtttcagcag cacaagctac atgtctgtcc aatcagaacg
     2221 cccaggagga aacaagaaaa tcagtgtctt tacatccaaa atgcaaagca gacaaatggg
     2281 catatcaggg aagaacatga caaaaagcac cagcatcagt ggagacatgt gctcactgga
     2341 gaagaatgat ggcagccagt ctgacactgc agtgggcacc ttgggcacca gtggcaaaaa
     2401 gcggcgctct agccttggtg ccaaaatggt agctatcgtt ggtctgtcac ggaaaagtcg
     2461 cagtgcttct cagctcagcc aaacggaagc aggaggtaaa aaactaagga gcactgtcca
     2521 aagaagtaca gaaacaggcc tggccgtgga aatgaggaac tggatgactc gacaggcaag
     2581 ccgagagtct acagatggta gcatgaacag ctacagctca gaaggaaatc tgattttccc
     2641 tggtgttcgc ttggcctctg atagccagtt cagtgatttc ctggatggcc ttggccctgc
     2701 tcagctagtg ggacgccaga ctctggcaac acctgcaatg ggtgacattc aagtaggaat
     2761 gatggacaaa aagggacagc tggaggtaga aatcatccgg gcccgtggcc ttgttgtaaa
     2821 accaggttcc aagacactgc cagcaccgta tgtaaaagtg tatctattag ataacggagt
     2881 ctgcatagcc aaaaagaaaa caaaagtggc aagaaaaacg ctggaacccc tttaccagca
     2941 gctattatct ttcgaagaga gtccacaagg aaaagtttta cagatcatcg tctggggaga
     3001 ttatggccgc atggatcaca aatcttttat gggagtggcc cagatacttt tagatgaact
     3061 agagctatcc aatatggtga tcggatggtt caaacttttc ccaccttcct ccctagtaga
     3121 tccaaccttg gctcctctga caagaagagc ttcccaatca tctctggaaa gttcaactgg
     3181 accttcttac tctcgttcat gtacagacta caaagaccat gacggtgatt ataaagatca
     3241 tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac
     3301 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     3361 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     3421 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
     3481 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
     3541 atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc
     3601 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     3661 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg
     3721 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     3781 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     3841 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     3901 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     3961 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     4021 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
     4081 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     4141 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     4201 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     4261 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
     4321 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
     4381 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     4441 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     4501 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     4561 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     4621 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     4681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     4741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     4801 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     4861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     4921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     4981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5041 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5341 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     5701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     5761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     5821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     5881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     5941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6481 acatgt
//