| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001361 |
| 产品用途: | 3' AAVLINK plasmid for RALGAPB expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RALGAPB |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG 5804 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5804)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5804
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2517
/codon_start=1
/label=RALGAPB
/translation="VRKRTKRF*RMWSLPELFSHIS*NFCFPLAGQ*MWADTLVGLGMF
LPVGLLIVVMMVKDLNKK*FPLKILELAFSMDRRRCCIMLMPLQKLLLWFLLLWSP*LI
HWKVTSRTKIVIQIWILCQEF*NSHP*HLSFSPIIQTILIPHRGSVPVPE*GSCLRVAL
FLPLDLRQEFL*SGWNAMMI*KTFPSQS**QRSVLVWKLLQIVALH*DLQLLKKKFLSS
SSTL*TLDYSG*KFKEPLENLIWSSLLWMG*LSAGELLAFW*GRL*LTFVEERDWKVTP
TVPPMSAGNRKSPTLSTSTGTSSWSQSFILHFSRRLDSRTAV"
misc_feature 2489..2494
/label=SDS
CDS 2524..2589
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2593..3024
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2737..2742
/label=SDS
repeat_region 3067..3207
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3067..3196
/label=AAV2 ITR
rep_origin 3282..3737
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4019..4123
/gene="bla"
/label=AmpR promoter
CDS 4124..4984
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5155..5743
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtca gaaaacgaac
1561 caagagattt taaagaatgt ggagtcttcc agaactgttc agccacattt cctagaattt
1621 ttgctttccc ttggctggtc agtagatgtg ggcagacacc ctggttggac tgggcatgtt
1681 tctaccagtt ggtctattaa ttgttgtgat gatggtgaag gatctcaaca agaagtgatt
1741 tcctctgaag atattggagc tagcattttc aatggacaga agaaggtgct gtattatgct
1801 gatgccctta cagaaattgc ttttgtggtt ccttctcctg tggagtcctt aactgattca
1861 ttggaaagta acatctcgga ccaagatagt gattcaaata tggatcttat gccaggaatt
1921 ctgaaacagc catccctgac acttgagctt ttccccaatc atacagacaa tcttaattcc
1981 tcacagaggc tcagtcccag ttccagaatg aggaagctgc ctcagggtcg ccctgttcct
2041 ccccttggac ctgagacaag agtttctgta gtctgggtgg aacgctatga tgatatagaa
2101 aactttcccc tctcagagct gatgacagag atcagtactg gtgtggaaac tactgcaaat
2161 agtagcactt cactgagatc tacaactctt gaaaaagaag ttcctgtcat cttcatccac
2221 cctttaaaca ctggattatt ccggataaaa attcaaggag ccactggaaa atttaatatg
2281 gtcatccctc ttgtggatgg gatgattgtc agcaggcgag ctcttggctt tctggtgagg
2341 cagactgtaa ttaacatttg tagaagaaag agactggaaa gtgactccta cagtcccccc
2401 catgtccgcc ggaaacagaa aatcaccgac attgtcaaca agtaccggaa caagcagctg
2461 gagccagagt tttatacttc acttttccag gaggttggac tcaagaactg cagttcttgt
2521 acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
2581 gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
2761 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
2821 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
2881 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
2941 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
3001 ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc
3061 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
3121 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
3181 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
3241 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
3301 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
3361 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
3421 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
3481 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
3541 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
3601 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
3661 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
3721 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
3781 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
3841 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
3901 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
3961 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
4021 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
4081 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
4141 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
4201 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
4261 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
4321 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
4381 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
4441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
4501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
4561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
4621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
4681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
4741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
4801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
4861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
4921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
4981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
5041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
5101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
5161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
5221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
5281 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
5341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
5401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
5461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
5521 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
5581 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
5641 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
5701 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
5761 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-RALGAPB-FLAG质粒载体应用举例
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