| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001478 |
| 产品用途: | 3' AAVLINK plasmid for PXDN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PXDN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PXDN-FLAG 6468 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6468)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6468
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3169
/codon_start=1
/label=PXDN
/translation="GIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGDHRANEQLG
LTSMHTLWFREHNRIATELLKLNPHWDGDTIYYETRKIVGAEIQHITYQHWLPKILGEV
GMRTLGEYHGYDPGINAGIFNAFATAAFRFGHTLVNPLLYRLDENFQPIAQDHLPLHKA
FFSPFRIVNEGGIDPLLRGLFGVAGKMRVPSQLLNTELTERLFSMAHTVALDLAAINIQ
RGRDHGIPPYHDYRVYCNLSAAHTFEDLKNEIKNPEIREKLKRLYGSTLNIDLFPALVV
EDLVPGSRLGPTLMCLLSTQFKRLRDGDRLWYENPGVFSPAQLTQIKQTSLARILCDNA
DNITRVQSDVFRVAEFPHGYGSCDEIPRVDLRVWQDCCEDCRTRGQFNAFSYHFRGRRS
LEFSYQEDKPTKKTRPRKIPSVGRQGEHLSNSTSAFSTRSDASGTNDFREFVLEMQKTI
TDLRTQIKKLESRLSTTECVDAGGESHANNTKWKKDACTICECKDGQVTCFVEACPPAT
CAVPVNIPGACCPVCLQKRAEEKP"
misc_feature 2760..2765
/label=SDS
CDS 3176..3241
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3257..3688
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3401..3406
/label=SDS
repeat_region 3731..3871
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3731..3860
/label=AAV2 ITR
rep_origin 3946..4401
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4683..4787
/gene="bla"
/label=AmpR promoter
CDS 4788..5648
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5819..6407
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca tcgtgcagcg
1561 gtccgggaag ccgctgctcc ccttcgccac cgggccgccc acggagtgca tgcgggacga
1621 gaacgagagc cccatcccct gcttcctggc cggggaccac cgcgccaacg agcagctggg
1681 cctgaccagc atgcacacgc tgtggttccg cgagcacaac cgcattgcca cggagctgct
1741 caagctgaac ccgcactggg acggcgacac catctactat gagaccagga agatcgtggg
1801 tgcggagatc cagcacatca cctaccagca ctggctcccg aagatcctgg gggaggtggg
1861 catgaggacg ctgggagagt accacggcta cgaccccggc atcaatgctg gcatcttcaa
1921 cgccttcgcc accgcggcct tcaggtttgg ccacacgctt gtcaacccac tgctttaccg
1981 gctggacgag aacttccagc ccattgcaca agatcacctc ccccttcaca aagctttctt
2041 ctctcccttc cggattgtga atgagggcgg catcgatccg cttctcaggg ggctgttcgg
2101 ggtggcgggg aaaatgcgtg tgccctcgca gctgctgaac acggagctca cggagcggct
2161 gttctccatg gcacacacgg tggctctgga cctggcggcc atcaacatcc agcggggccg
2221 ggaccacggg atcccaccct accacgacta cagggtctac tgcaatctat cggcggcaca
2281 cacgttcgag gacctgaaaa atgagattaa aaaccctgag atccgggaga aactgaaaag
2341 gttgtatggc tcgacactca acatcgacct gtttccggcg ctcgtggtgg aggacctggt
2401 gcctggcagc cggctgggcc ccaccctgat gtgtcttctc agcacacagt tcaagcgcct
2461 gcgagatggg gacaggttgt ggtatgagaa ccctggggtg ttctccccgg cccagctgac
2521 tcagatcaag cagacgtcgc tggccaggat cctatgcgac aacgcggaca acatcacccg
2581 ggtgcagagc gacgtgttca gggtggcgga gttccctcac ggctacggca gctgtgacga
2641 gatccccagg gtggacctcc gggtgtggca ggactgctgt gaagactgta ggaccagggg
2701 gcagttcaat gccttttcct atcatttccg aggcagacgg tctcttgagt tcagctacca
2761 ggaggacaag ccgaccaaga aaacaagacc acggaaaata cccagtgttg ggagacaggg
2821 ggaacatctc agcaacagca cctcagcctt cagcacacgc tcagatgcat ctgggacaaa
2881 tgacttcaga gagtttgttc tggaaatgca gaagaccatc acagacctca gaacacagat
2941 aaagaaactt gaatcacggc tcagtaccac agagtgcgtg gatgccgggg gcgaatctca
3001 cgccaacaac accaagtgga aaaaagatgc atgcaccatt tgtgaatgca aagacgggca
3061 ggtcacctgc ttcgtggaag cttgcccccc tgccacctgt gctgtccccg tgaacatccc
3121 aggggcctgc tgtccagtct gcttacagaa gagggcggag gaaaagccct gtacagacta
3181 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
3241 gtaagaattc tgatgactcg agataatcaa cctctggatt acaaaatttg tgaaagattg
3301 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
3361 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
3421 ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
3481 cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga tgctattgct
3541 ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt tatttgtaac
3601 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
3661 tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca cgtgcggacc
3721 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
3781 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
3841 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
3901 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
3961 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
4021 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
4081 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
4141 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
4201 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
4261 ctggaacaac actcaactct atctcgggct attcttttga tttataaggg attttgccga
4321 tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
4381 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
4441 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
4501 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
4561 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
4621 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
4681 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
4741 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
4801 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
4861 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
4921 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
4981 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
5041 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
5101 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
5161 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
5221 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
5281 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5341 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5401 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5461 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5521 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5581 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
5641 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
5701 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
5761 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
5821 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
5881 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
5941 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
6001 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
6061 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
6121 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
6181 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
6241 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6301 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6361 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6421 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
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