| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001409 |
| 产品用途: | 3' AAVLINK plasmid for PHIP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHIP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PHIP-FLAG 7078 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7078)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7078
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3791
/codon_start=1
/label=PHIP
/translation="VCHR*CLVVWNNRKPGTSST*VP**SVSMLQCLLGQWRYRKDESL
GYGAYT**CCIS*RTRYQCSFN*W*VQITNL*TS*WRMGYQSQG*RM*KNCGRNKPVDD
TRYCLSICGPRGSASLSHVLHSSGISNGSKYN*TKTGKQVLQAGFFPNVGSSIYRA*YT
NI**AWKPYCEIC*IRD*SSSTFYKGSDLL*HNSTL*FNEEESFV*F*G*RERC*CARN
FYSKKEGPSA*KKIT**SPVLRYSSMEETV*RIVKSHISM*RFRAFPSAGRSP*ISRLQ
RHH*HSNGFCYR*RNFRGWEL*VTNGVM*RCQTYFQ*FQSIYTKQKIKDLQHEFALVCI
L*RTH*FSFIRL*ICSSFS*KKYHNQKEEEKKQKQLCFQ*CCIKP*KEKKDLKTPAKIR
KLYLCILYTYTINTAKTQCCSDKR*NRI*FCGSNQKQPSGCRSSCH*ATIYFFSCKDFY
YKS*CICNTRENNTREFCETFQSFEYSFQSWSIQF*SWH*E*FCKRKHGKGKASQT*NE
VICTPKGVHSFKVISCH*ARRL*EQRSCTRNHSSKWPWRTAIKTCEEGTWKET*SRS*Y
Q*W*NYTQEKG*KAQKATVCKARRFRAK*CASHQR*STSFFNMQFSF*N**CKGRFVTE
KESWR*EAQKEDEDTKIRCRSPSPCKCQSVKEK*PKKDR*SYR*GRRV*RTQRL*TPHE
N*KSRSKDSFL**G*L*RGAKAAVVRRHLFNFWNF**RTSPKVD*KSKS*FNWL"
misc_feature 2646..2651
/label=SDS
CDS 3798..3863
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3867..4298
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4011..4016
/label=SDS
repeat_region 4341..4481
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4341..4470
/label=AAV2 ITR
rep_origin 4556..5011
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5293..5397
/gene="bla"
/label=AmpR promoter
CDS 5398..6258
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6429..7017
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtct gtcatagatg
1561 atgcctggtg gtttggaaca atcgaaagcc aggaacctct tcaacctgag taccctgata
1621 gtctgtttca atgctacaat gtttgctggg acaatggaga tacagaaaag atgagtcctt
1681 gggatatgga gcttatacct aataatgctg tatttcctga agaactaggt accagtgttc
1741 ctttaactga tggtgagtgc agatcactaa tctataaacc tcttgatgga gaatggggta
1801 ccaatcccag ggatgaagaa tgtgaaagaa ttgtggcagg aataaaccag ttgatgacac
1861 tagatattgc ctcagcattt gtggcccccg tggatctgca agcctatccc atgtattgca
1921 cagtagtggc atatccaacg gatctaagta caattaaaca aagactggaa aacaggtttt
1981 acaggcgggt ttcttcccta atgtgggaag ttcgatatat agagcataat acacgaacat
2041 ttaatgagcc tggaagccct attgtgaaat ctgctaaatt cgtgactgat cttcttctac
2101 attttataaa ggatcagact tgttataaca taattccact ttataattca atgaagaaga
2161 aagttttgtc tgattctgag gatgaagaga aagatgctga tgtgccagga acttctactc
2221 gaaaaaggaa ggaccatcag cctagaagaa gattacgtaa tagagcccag tcttacgata
2281 ttcaagcatg gaagaaacag tgtgaagaat tgttaaatct catatttcaa tgtgaagatt
2341 cagagccttt ccgtcagccg gtagatctcc ttgaatatcc agactacaga gacatcattg
2401 acactccaat ggattttgct accgttagag aaactttaga ggctgggaat tatgagtcac
2461 caatggagtt atgtaaagat gtcagactta ttttcagtaa ttccaaagca tatacaccaa
2521 gcaaaagatc aaggatttac agcatgagtt tgcgcttgtc tgcattcttt gaagaacaca
2581 ttagttcagt tttatcagat tataaatctg ctcttcgttt tcataaaaga aataccataa
2641 ccaaaaggag gaagaaaaga aacagaagca gctctgtttc cagtagtgct gcatcaagcc
2701 ctgaaaggaa aaaaaggatc ttaaaacccc agctaaaatc agaaagctct acctctgcat
2761 tctctacacc tacacgatca ataccgccaa gacacaatgc tgctcagata aacggtaaaa
2821 cagaatctag ttctgtggtt cgaaccagaa gcaaccgagt ggttgtagat ccagttgtca
2881 ctgagcaacc atctacttct tcagctgcaa agacttttat tacaaaagct aatgcatctg
2941 caataccagg gaaaacaata ctagagaatt ctgtgaaaca ttccaaagct ttgaatactc
3001 tttccagtcc tggtcaatcc agttttagtc atggcactag gaataattct gcaaaagaaa
3061 acatggaaaa ggaaaagcca gtcaaacgta aaatgaagtc atctgtactc ccaaaggcgt
3121 ccactctttc aaagtcatca gctgtcattg agcaaggaga ttgtaagaac aacgctcttg
3181 taccaggaac cattcaagta aatggccatg gaggacagcc atcaaaactt gtgaagaggg
3241 gacctggaag gaaacctaaa gtagaagtta ataccaatag tggtgaaatt atacacaaga
3301 aaaggggtag aaagcccaaa aagctacagt atgcaaagcc agaagattta gagcaaaata
3361 atgtgcatcc catcagagat gaagtacttc cttcttcaac atgcaatttt ctttctgaaa
3421 ctaataatgt aaaggaagat ttgttacaga aaaagaatcg tggaggtagg aagcccaaaa
3481 ggaagatgaa gacacaaaaa ttagatgcag atctcctagt ccctgcaagt gtcaaagtgt
3541 taaggagaag taaccgaaaa aagatagatg atcctataga tgaggaagaa gagtttgaag
3601 aactcaaagg ctctgaaccc cacatgagaa ctagaaatca aggtcgaagg acagctttct
3661 ataatgagga tgactctgaa gaggagcaaa ggcagctgtt gttcgaagac acctctttaa
3721 cttttggaac ttctagtaga ggacgagtcc gaaagttgac tgaaaaagca aaagctaatt
3781 taattggttg gtgtacagac tacaaagacc atgacggtga ttataaagat catgacatcg
3841 actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt acaaaatttg
3901 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
3961 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
4021 taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
4081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg aaatttgtga
4141 tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat gctattgctt
4201 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
4261 tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac cggtgtggca
4321 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
4381 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
4441 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
4501 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
4561 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
4621 cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
4681 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
4741 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
4801 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
4861 ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga tttataaggg
4921 attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
4981 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
5041 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
5101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
5161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
5221 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
5281 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
5341 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
5401 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5461 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5521 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5581 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5641 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5701 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5761 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5821 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5881 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5941 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
6001 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
6061 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
6121 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
6181 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
6241 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
6301 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
6361 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6421 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6481 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6541 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6601 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6661 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6721 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6781 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6841 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6901 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6961 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
7021 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
