| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001475 |
| 产品用途: | 3' AAVLINK plasmid for PCDH11X expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PCDH11X |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-PCDH11X-HA 6813 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6813)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6813
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3496
/codon_start=1
/label=PCDH11X
/translation="VIAVDNDTGMNAEVRYSIVGGNTRDLFAIDQETGNITLMEKCDVT
DLGLHRVLVKANDLGQPDSLFSVVIVNLFVNESVTNATLINELVRKSTEAPVTPNTEIA
DVSSPTSDYVKILVAAVAGTITVVVVIFITAVVRCRQAPHLKAAQKNKQNSEWATPNPE
NRQMIMMKKKKKKKKHSPKNLLLNFVTIEETKADDVDSDGNRVTLDLPIDLEEQTMGKY
NWVTTPTTFKPDSPDLARHYKSASPQPAFQIQPETPLNSKHHIIQELPLDNTFVACDSI
SKCSSSSSDPYSVSDCGYPVTTFEVPVSVHTRPPMKEVVRSCTPMKESTTMEIWIHPQP
QRKSEGKVAGKSQRRVTFHLPEGSQESSSDGGLGDHDAGSLTSTSHGLPLGYPQEEYFD
RATPSNRTEGDGNSDPESTFIPGLKKAAEITVQPTVEEASDNCTQECLIYGHSDACWMP
ASLDHSSSSQAQASALCHSPPLSQASTQHHSPRVTQTIALCHSPPVTQTIALCHSPPPI
QVSALHHSPPLVQATALHHSPPSAQASALCYSPPLAQAAAISHSSPLPQVIALHRSQAQ
SSVSLQQGWVQGADGLCSVDQGVQGSATSQFYTMSERLHPSDDSIKVIPLTTFTPRQQA
RPSRGDSPIMEEHPL"
misc_feature 1603..1608
/label=SDS
misc_feature 2496..2501
/label=SDS
misc_feature 2727..2732
/label=SDS
misc_feature 3122..3127
/label=SDS
misc_feature 3194..3199
/label=SDS
CDS 3512..3538
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3542..3568
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 3572..3598
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
terminator 3602..4033
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3746..3751
/label=SDS
repeat_region 4076..4216
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4076..4205
/label=AAV2 ITR
rep_origin 4291..4746
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5028..5132
/gene="bla"
/label=AmpR promoter
CDS 5133..5993
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6164..6752
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtaa ttgctgttga
1561 caatgacact ggcatgaatg cagaggttcg ttacagcatt gtaggaggaa acacaagaga
1621 tctgtttgca atcgaccaag aaacaggcaa cataacattg atggagaaat gtgatgttac
1681 agaccttggt ttacacagag tgttggtcaa agctaatgac ttaggacagc ctgattctct
1741 cttcagtgtt gtaattgtca atctgttcgt gaatgagtcg gtgaccaatg ctacactgat
1801 taatgaactg gtgcgcaaaa gcactgaagc accagtgacc ccaaatactg agatagctga
1861 tgtatcctca ccaactagtg actatgtcaa gatcctggtt gcagctgttg ctggcaccat
1921 aactgtcgtt gtagttattt tcatcactgc tgtagtaaga tgtcgccagg caccacacct
1981 taaggctgct cagaaaaaca agcagaattc tgaatgggct accccaaacc cagaaaacag
2041 gcagatgata atgatgaaga aaaagaaaaa gaagaagaag cattccccta agaacttgct
2101 gcttaatttt gtcactattg aagaaactaa ggcagatgat gttgacagtg atggaaacag
2161 agtcacacta gaccttccta ttgatctaga agagcaaaca atgggaaagt acaattgggt
2221 aactacacct actactttca agcccgacag ccctgatttg gcccgacact acaaatctgc
2281 ctctccacag cctgccttcc aaattcagcc tgaaactccc ctgaattcga agcaccacat
2341 catccaagaa ctgcctctcg ataacacctt tgtggcctgt gactctatct ccaagtgttc
2401 ctcaagcagt tcagatccct acagcgtttc tgactgtggc tatccagtga cgaccttcga
2461 ggtacctgtg tccgtacaca ccagaccgcc aatgaaggag gttgtgcgat cttgcacccc
2521 catgaaagag tctacaacta tggagatctg gattcatccc caaccacagc ggaaatctga
2581 agggaaagtg gcaggaaagt cccagcggcg tgtcacattt cacctgccag aaggctctca
2641 ggaaagcagc agtgatggtg gactgggaga ccatgatgca ggcagcctta ccagcacatc
2701 tcatggcctg ccccttggct atcctcagga ggagtacttt gatcgtgcta cacccagcaa
2761 tcgcactgaa ggggatggca actccgatcc tgaatctact ttcatacctg gactaaagaa
2821 agctgcagaa ataactgttc aaccaactgt ggaagaggcc tctgacaact gcactcaaga
2881 atgtctcatc tatggccatt ctgatgcctg ctggatgccg gcatctctgg atcattccag
2941 ctcttcgcaa gcacaggcct ctgctctatg ccacagccca ccactgtcac aggcctctac
3001 tcagcaccac agcccacgag tgacacagac cattgctctc tgccacagcc ctccagtgac
3061 acagaccatc gcattgtgcc acagcccacc accgatacag gtgtctgctc tccaccacag
3121 tcctcctcta gtgcaggcta ctgcacttca ccacagccca ccatcagcac aggcctcagc
3181 cctctgctac agccctcctt tagcacaggc tgctgcaatc agccacagct ctcctctgcc
3241 acaggttatt gccctccatc gtagtcaggc ccaatcatca gtcagtttgc agcaaggttg
3301 ggtgcaaggt gctgatgggc tatgctctgt tgatcaggga gtgcaaggta gtgcaacatc
3361 tcagttttac accatgtctg aaagacttca tcccagtgat gattcaatta aagtcattcc
3421 tttgacaacc ttcactccac gccaacaggc cagaccgtcc agaggtgatt cccccattat
3481 ggaagaacat cccttgggag gtggatctgg ttacccgtat gatgttccgg attacgctgg
3541 ctatccctac gacgtgcccg actatgccgg gtacccctat gacgtcccag actacgcata
3601 actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
3661 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
3721 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg
3781 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
3841 gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
3901 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
3961 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
4021 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
4081 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
4141 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
4201 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
4261 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
4321 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
4381 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
4441 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
4501 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
4561 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
4621 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
4681 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
4741 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
4801 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
4861 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
4921 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
4981 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
5041 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
5101 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
5161 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
5221 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
5281 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
5341 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
5401 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
5461 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
5521 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
5581 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
5641 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
5701 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
5761 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
5821 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
5881 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
5941 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
6001 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
6061 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
6121 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
6181 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
6241 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
6301 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
6361 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
6421 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
6481 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
6541 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
6601 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
6661 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
6721 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
6781 tcctggcctt ttgctggcct tttgctcaca tgt
//
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