| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001403 |
| 产品用途: | 5' AAVLINK plasmid for NUP155 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NUP155 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NUP155-FLAG 6861 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6861)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6861
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3574
/codon_start=1
/label=NUP155
/translation="GLQEFLDRNSQFAGGPLGNPNTTAKVQQRLIGFMRPENGNPQQMQ
QELQRKFHEAQLSEKISLQAIQQLVRKSYQALALWKLLCEHQFTIIVAELQKELQEQLK
ITTFKDLVIRDKELTGALIASLINCYIRDNAAVDGISLHLQDICPLLYSTDDAICSKAN
ELLQRSRQVQNKTEKERMLRESLKEYQKISNQVDLSNVCAQYRQVRFYEGVVELSLTAA
EKKDPQGLGLHFYKHGEPEEDIVGLQAFQERLNSYKCITDTLQELVNQSKAAPQSPSVP
KKPGPPVLSSDPNMLSNEEAGHHFEQMLKLSQRSKDELFSIALYNWLIQVDLADKLLQV
ASPFLEPHLVRMAKVDQNRVRYMDLLWRYYEKNRSFSNAARVLSRLADMHSTEISLQQR
LEYIARAILSAKSSTAISSIAADGEFLHELEEKMEVARIQLQIQETLQRQYSHHSSVQD
AVSQLDSELMDITKLYGEFADPFKLAECKLAIIHCAGYSDPILVQTLWQDIIEKELSDS
VTLSSSDRMHALSLKIVLLGKIYAGTPRFFPLDFIVQFLEQQVCTLNWDVGFVIQTMNE
IGVPLPRLLEVYDQLFKSRDPFWNRMKKPLHLLDCIHVLLIRYVENPSQVLNCERRRFT
NLCLDAVCGYLVELQSMSSSVAVQAITGNFKSLQAKLERLH"
misc_feature 1585..1590
/label=SDS
CDS 3581..3646
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3650..4081
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3794..3799
/label=SDS
repeat_region 4124..4264
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4124..4253
/label=AAV2 ITR
rep_origin 4339..4794
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5076..5180
/gene="bla"
/label=AmpR promoter
CDS 5181..6041
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6212..6800
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggtt tgcaggaatt
1561 tctagacaga aactcccagt ttgcaggagg accattagga aatccaaata ctactgctaa
1621 agtgcagcag aggctgatag gattcatgcg tcctgaaaac ggaaatcccc agcaaatgca
1681 acaggaactg cagaggaagt ttcatgaggc tcaactaagt gaaaagattt cacttcaggc
1741 aattcagcag ttggttcgaa aatcatatca ggctctggct ttatggaaac ttctttgtga
1801 acatcaattc actatcattg tggcagaact tcagaaggaa cttcaagagc agctgaagat
1861 caccaccttt aaagatcttg taatcaggga caaagaactc acaggggcat taattgcttc
1921 tcttatcaac tgctacatca gagataatgc cgctgttgat ggcattagtt tacatttaca
1981 ggatatctgc ccacttctat atagcactga tgatgcaatt tgttctaagg caaatgagct
2041 tctccagcgt tcccgacaag ttcaaaataa gactgaaaaa gaaagaatgt taagggaatc
2101 attaaaggaa tatcaaaaaa ttagcaatca agtggacctt tccaatgttt gtgctcagta
2161 tagacaagtg agattttatg agggtgtggt ggaactttct cttacggctg cagagaaaaa
2221 agatcctcaa ggtcttgggc ttcatttcta taaacatgga gaaccagaag aagacatagt
2281 tggacttcag gccttccaag aaagattaaa cagttacaaa tgcattacag acacacttca
2341 agaactggta aatcaaagta aggccgctcc tcagtctccc agtgtaccca aaaaacctgg
2401 tcctccagtg ttgtcatctg atccaaatat gctgagtaat gaagaagcag gacatcattt
2461 tgaacaaatg cttaaattgt cacagcgatc caaggatgag ctctttagta ttgcccttta
2521 taattggcta atacaagtcg accttgcaga taagctgcta caggttgctt ctccatttct
2581 ggagccacat ctagtccgaa tggccaaagt tgatcaaaac agagttcgtt atatggattt
2641 actctggcgg tattacgaga agaacagaag tttcagtaat gctgctcgtg tactgtccag
2701 actggctgac atgcatagca cagaaatttc acttcagcag cgactagagt acattgctcg
2761 agccattctt agtgccaaaa gttccactgc catttcatca atagctgccg atggtgaatt
2821 tcttcatgaa ttagaagaaa aaatggaggt tgctaggatc caacttcaga tacaggagac
2881 actacaaagg cagtattccc atcattcttc tgtacaggat gcagtttctc agctggattc
2941 tgagctgatg gacataacta agctttatgg ggaatttgct gacccattta aacttgcaga
3001 gtgcaaactt gcaataattc attgtgccgg ttattcagac cctatattgg tgcagacact
3061 ttggcaagat atcatagaga aagaattgag tgacagtgtg acattgagct cctcggatag
3121 aatgcatgct cttagtctca agattgttct ccttggcaaa atttatgctg gcacaccacg
3181 cttctttcct ttagatttta ttgtacagtt tttagaacag caggtttgta ctttgaactg
3241 ggatgtgggc ttcgtaatac agacaatgaa tgaaattgga gtaccattac ctagactact
3301 agaagtttat gatcagttgt tcaaatcacg ggatccattc tggaacagaa tgaagaagcc
3361 actgcacctt ttggattgta tacatgtatt attgataaga tatgttgaga atcccagcca
3421 agttttaaat tgtgaaagga gaagatttac aaatctctgc ctggatgctg tttgtggtta
3481 ccttgttgag ctccagtcta tgagctcgtc agtagcagta caagccatca ctgggaattt
3541 taaatctctt caagctaaat tagaacggct tcattgtaca gactacaaag accatgacgg
3601 tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat
3661 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
3721 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
3781 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
3841 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
3901 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
3961 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
4021 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
4081 aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
4141 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
4201 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
4261 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
4321 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
4381 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
4441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
4501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
4561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
4621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
4681 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
4741 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
4801 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
4861 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
4921 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
4981 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
5041 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
5101 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
5161 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
5221 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
5281 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
5341 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
5401 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
5461 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
5521 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
5581 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
5641 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
5701 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
5761 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
5821 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
5881 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
5941 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
6001 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
6061 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
6121 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
6181 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
6241 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
6301 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
6361 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6421 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6481 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
6541 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
6601 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
6661 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6721 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6781 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
6841 gctggccttt tgctcacatg t
//
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