| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001460 |
| 产品用途: | 3' AAVLINK plasmid for NINL expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NINL |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NINL-FLAG 6389 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6389)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6389
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3102
/codon_start=1
/label=NINL
/translation="VMAESLRRRQEPGLAAGKPRTQKLRRARPPPLPRHPTAPQRGGHA
CSPVEWMGILSQRSQSLSARAQRGWSSLEPGSCLCWEQRETPRKPSHGCGSHP*GRPLR
AGDRLRGYRPFRKSEHEAGAGAPRSRPRSSRPGPRAPWSLGVTSTVWRLPGEGPCHPTS
SSQTRRVPGRSSLLPQKRGRPK*RWRERRMTWKPNFYIWKTSSGLWRNM*I*ERTTDWS
SIDFLKKTLC*KTIWEGFGKSLKLQKVLTMHRGRKLRF*RKTRKRPALRWRCSTDRIRT
TRINYPSSMSGFFNWDRRLLPTRPKTRSIV*PFRC*HRAWRRWFAVGSSRVTKSKNLEL
NLNA*IRNIRACSCHGQS*PRPLRKVKTRCRELT*G*GRPRPSTCRRSGWCPRTVWPSC
IACSAFRESRPGGAWMHSGKNMRNS*KPQKSGWKRRR*F*RIWKCSSKRKWIS*RSSLK
RTRSPTCC*RSCTWRTPTW*EHFRPPRRSSEAPRNKAASWKKKFALSTNSSVGLPPQPS
L"
misc_feature 1565..1570
/label=SDS
misc_feature 2438..2443
/label=SDS
misc_feature 2510..2515
/label=SDS
misc_feature 2702..2707
/label=SDS
misc_feature 2776..2781
/label=SDS
CDS 3109..3174
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3178..3609
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3322..3327
/label=SDS
repeat_region 3652..3792
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3652..3781
/label=AAV2 ITR
rep_origin 3867..4322
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4604..4708
/gene="bla"
/label=AmpR promoter
CDS 4709..5569
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5740..6328
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtga tggcagagag
1561 tctgaggagg cggcaggagc cgggcctcgc cgcaggcaag cccaggacac agaagctacg
1621 cagagcccgg cccccgcccc tgccccggca tcccacggcc cctcagagag gtggtcacgc
1681 atgcagccct gtggagtgga tggggatatt gtcccaaagg agccagagcc tttcggcgcg
1741 agcgcagcgg ggctggagca gcctggagcc cgggagctgc ctctgctggg aacagagaga
1801 gacgcctcgc aaacccagcc acggatgtgg gagccacccc tgaggccggc cgcttcgtgc
1861 aggggacagg ctgagaggct acaggccatt caggaagagc gagcacgaag ctggagcagg
1921 ggcacccagg agcaggcctc ggagcagcag gcccgggccg agggcgccct ggagcctggg
1981 tgtcacaagc acagtgtgga ggttgccagg agagggtcct tgccatccca cctccagctc
2041 gcagacccgc agggttcctg gcaggagcag cttgctgccc cagaagaggg ggagaccaaa
2101 atagcgctgg agagagagaa ggatgacatg gaaaccaaac ttctacatct ggaagacgtc
2161 gtccgggctc tggagaaaca tgtagatttg agagagaacg acagactgga gttccataga
2221 ctttctgaag aaaacacttt gttgaaaaac gatctgggaa gggttcggca agagcttgaa
2281 gctgcagaaa gtactcacga tgcacagagg aaggaaattg aggttttaaa gaaagacaag
2341 gaaaaggcct gctctgagat ggaggtgctc aacagacaga atcagaacta caaggatcaa
2401 ttatcccagc tcaatgtcag ggttcttcaa ctgggacagg aggcttctac ccaccaggcc
2461 caaaacgagg agcatcgtgt gaccattcag atgttaacac agagcctgga ggaggtggtt
2521 cgcagtgggc agcagcagag tgaccaaatc caaaaactta gagttgaact tgaatgcctg
2581 aatcaggaac atcagagcct gcagctgcca tggtcagagc tgacccagac ccttgaggaa
2641 agtcaagacc aggtgcaggg agctcacctg aggctgaggc aggcccaggc ccagcacttg
2701 caggaggtcc ggctggtgcc ccaggaccgt gtggccgagc tgcatcgcct gctcagcctt
2761 cagggagagc aggccaggag gcgcctggat gcacagcggg aagaacatga gaaacagctg
2821 aaagccacag aagagcgggt ggaagaggcg gagatgattc tgaagaatat ggaaatgctc
2881 ctccaagaga aagtggataa gctgaaggag cagtttgaaa agaacacgaa gtccgacctg
2941 ctgctgaagg agctgtacgt ggagaacgcc cacctggtga gagcacttca ggccaccgag
3001 gagaagcagc gaggcgccga gaaacaaagc cgcctcttgg aagaaaaagt tcgcgctctc
3061 aacaaactcg tcagtaggat tgcccccgca gccctctctg tgtgtacaga ctacaaagac
3121 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtaactc
3181 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
3241 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
3301 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
3361 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3421 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
3481 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
3541 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
3601 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
3661 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
3721 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
3781 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
3841 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
3901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
3961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4021 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4081 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
4201 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
4261 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
4321 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
4381 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
4441 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4501 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
4561 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
4621 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
4681 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
4741 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
4801 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
4861 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
4921 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
4981 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
5041 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
5101 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
5161 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5221 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
5281 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
5341 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
5401 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
5461 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
5521 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
5581 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
5641 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
5701 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
5761 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
5821 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
5881 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
5941 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6001 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
6061 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6121 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6181 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6241 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
6301 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
6361 ggccttttgc tggccttttg ctcacatgt
//
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