| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001367 |
| 产品用途: | 3' AAVLINK plasmid for NEXMIF expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
NEXMIF
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEXMIF-FLAG 6664 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6664)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6664
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3377
/codon_start=1
/label=NEXMIF
/translation="GDSPNPIQ*IWSLPSSLHGK*PHTYNIQSNLSQ*WWQ*LQQGPV*
LHARYPTPI**LLPIMSL**WRDLLSFPAGASQYG*WSAL*L*FNGPTLCQLK*LLLLK
LEVL*KGWR**YH**LPGPLQP*AGDPAKH**DSTTKGVH*PPGYIQLHP*QIPPLFPF
RDVPTGHP*SFPSNYQM*EYEDTRNTKGVPRGCPRTIGQCGKNQVGLQYPFTAGPNGGW
IYFK*SPVSVPYVQ**GFCQPAPKKPLPVNI**SIWSN*YQQQSVKIKKEKFTQQEWGY
EPKLFSEKHQEKIPQR*QQGD*KTTWQKLPPGP*VHKEREIHGCHQWRENANWHWPWGK
PNQHHILHWKDIG*MYPTWWPYGLYEDAKSKGTFWRLGLGEGEQPRLE*YEHGHQHQQP
SG**PTGISGAFLYLVQHCLWYGRCAEIHDGLHRAPLGTHGAPWGSQHILLP*VQ*SKI
KNPQNIGWDTTGV*EKDQQWVPRSH*ESQVNQGCEQKQWENSNR*SWSCKHAWL*RGLS
LYLL**KV**HEHFRQ*RTDTQKVVSSQIQLQGPERREM*GKAHGARTGPQG*VWDSFF
*KTEGFRLQSPKSRNNLLGFTCV*RRDSHFPERH"
misc_feature 1982..1987
/label=SDS
CDS 3384..3449
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3453..3884
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3597..3602
/label=SDS
repeat_region 3927..4067
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3927..4056
/label=AAV2 ITR
rep_origin 4142..4597
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4879..4983
/gene="bla"
/label=AmpR promoter
CDS 4984..5844
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6015..6603
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag atagccccaa
1561 cccaatccag tgaatttgga gcctcccaag tagtctccat ggaaaataac ctcacaccta
1621 caacatacaa tccaatctgt ctcaatagtg gtggcagtaa ttgcaacaag gtcctgtatg
1681 actccatgca agatacccaa ctcccatctg atgactctta ccaattatgt cactttaata
1741 atggagagat ctgctttcct ttccagcagg ggccagtcaa tatggatgat ggtcggctct
1801 ttagctttga ttcaatggcc ccactctctg tcagctcaag taattattgc tccttaagct
1861 tgaagtcctg tgaaaaggat ggcgatgatg atatcactga tgacttcctg gcccattgca
1921 gccctaagct ggtgatccag caaagcattg atgagatagc accactaaag gagtccactg
1981 acctcctgga tatatccaac ttcacccctg acaaattccg ccactcttcc ctttcagaga
2041 tgtccccacc ggacacccct agtctttccc ctcaaattac cagatgtgag agtatgaaga
2101 cactaggaac actaaagggg ttccaagagg gtgtcccagg accattggac agtgtggaaa
2161 aaatcaagtg ggactgcagt accctttcac ggcaggtcca aatggaggat ggatttactt
2221 taaataatca ccagtttcag ttccatatgt tcaatgatga ggattctgtc agcctgctcc
2281 aaaaaaaccc ttgcctgtca acatttaatg atccatctgg tcaaattagt accaacaaca
2341 aagtgtcaaa atcaagaaag aaaagttcac ccagcaagag tggggctatg aaccaaagct
2401 cttctcagaa aaacaccagg aaaaaatccc tcaaaggtaa caacaagggg attgaaaaac
2461 cacctggcaa aaactcccgc caggtcccta agtccacaaa gaaagggaaa tacatggctg
2521 ccatcaatgg agagaaaatg caaattggca ttggccgtgg gggaagccaa accaacacca
2581 tatcctccac tggaaagaca ttggctgaat gtatccaaca tggtggccct atggcctcta
2641 tgaagatgcc aagtcaaaag ggactttctg gagattgggc cttggggaag gagagcagcc
2701 caggctggag tgatatgagc atgggcacca acaccaacag ccttctggat gatgaccaac
2761 gggaatttca ggagccttcc tatatcttgt ccaacattgc ctctggtatg gcagatgtgc
2821 agagattcat gatggcctcc atagagcccc tttgggaacc catggagcac catggggatc
2881 ccaacatatt ctactcccct gagtccaata gtctaaaatt aaaaaccctc aaaatattgg
2941 ctgggacacc acaggagtct aagaaaaaga tcaacagtgg gtcccaagga gccactaaga
3001 atcacaggtc aatcaagggt gtgagcaaaa gcaatgggaa aacagcaata ggtgatcctg
3061 gtcgtgcaaa catgcctggt tataacgagg actctcgctc taccttcttt gataaaaagt
3121 atagtaacat gagcacttta ggcaataacg gaccgacaca caaaaagttg tatcgtcaca
3181 aatccagctc caaggccctg agagacgaga aatgtaaggg aaagcacatg gagcgagaac
3241 aggtccacaa ggatgagtct gggacagctt cttttgaaaa actgagggat tccgactaca
3301 atctcctaaa agcagaaaca accttttggg ttttacctgt gtttgaagaa gagactcgca
3361 ttttccagaa agacatttgt acagactaca aagaccatga cggtgattat aaagatcatg
3421 acatcgacta caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa
3481 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
3541 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
3601 cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg
3661 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat
3721 ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta
3781 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
3841 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt
3901 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
3961 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
4021 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
4081 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
4141 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
4201 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4261 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4321 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
4381 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
4441 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
4501 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
4561 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
4621 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
4681 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
4741 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
4801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
4861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
4921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
4981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
5041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
5101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
5161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
5221 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
5281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
5341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
5401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
5461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
5521 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
5581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
5641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
5701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
5761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
5821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
5881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
5941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
6001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
6061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
6121 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
6181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
6421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
6481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
6541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
6601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
6661 atgt
//
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