| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001364 |
| 产品用途: | 5' AAVLINK plasmid for MBD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
SCP1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
MBD5
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-MBD5-FLAG 6354 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6354)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6354
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3067
/codon_start=1
/label=MBD5
/translation="GYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRI
STQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKA
VVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSVVSAVIHGRNMG
GVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNR
WKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNN
YKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLEN
SLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVG
DLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNR
KSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR"
misc_feature 3050..3055
/label=SDS
CDS 3074..3139
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3143..3574
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3287..3292
/label=SDS
repeat_region 3617..3757
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3617..3746
/label=AAV2 ITR
rep_origin 3832..4287
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4569..4673
/gene="bla"
/label=AmpR promoter
CDS 4674..5534
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5705..6293
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggt accagaatct
1561 ccaggcgttc caaggacagt ccacaattcc ttgcccagct aacaataacc ccatggcttg
1621 tctgtttcag aactttcagg tgagaatgca ggaagatgca gctctcctaa acaaaagaat
1681 aagcactcag cctgggctca cagcacttcc tgagaatcca aacactacac ttccaccttt
1741 tcaagataca ccttgtgagt tgcaaccgag gattgaccca tctcttggtc aacaggtgaa
1801 ggatggcctc gttgtgggtg gcccaggtga tgcttccgta gatgccattt acaaagcagt
1861 tgtcgatgca gccagcaaag gaatgcaggt tgtcatcacc actgcagtca acagtacaac
1921 tcagatcagc cccattccag ctctgagtgc catgagtgcc ttcactgcct caattggtga
1981 cccattaaat ctctccagtg ttgtcagtgc ggtcattcat ggacggaaca tgggaggtgt
2041 tgatcatgat ggtaggctga ggaattcaag aggggctcgg ctgcccaaga atctagacca
2101 tgggaaaaat gtgaacgaag gagatgggtt tgaatatttc aagtcagcaa gttgccacac
2161 atccaaaaaa cagtgggacg gggagcaaag ccccagaggg gagcgaaaca ggtggaagta
2221 cgaggaattt ttagatcatc caggccatat ccacagtagt ccttgtcatg aaaggcccaa
2281 caatgtctct acactgccat ttctgcctgg ggaacagcac ccaatactgt taccaccaag
2341 aaactgtcca ggggataaaa ttctagagga aaatttcagg tataataact acaaaagaac
2401 tatgatgagt tttaaggaga gactagagaa cactgtggaa agatgtgcac acataaatgg
2461 gaatagacct cgacagagtc ggggatttgg agagctgcta agcactgcaa agcaagacct
2521 ggtcctagag gagcagtctc caagttcctc aaatagtttg gaaaattctc tggtcaaaga
2581 ctacatccat tacaatggag actttaatgc caaaagcgtt aatgggtgtg tgcctagccc
2641 ttcagatgct aaaagcatta gtagtgaaga tgacctaagg aacccagact ccccctcttc
2701 aaatgaattg atacattata gaccaaggac gttcaatgtt ggcgacttgg tctggggcca
2761 aatcaaagga ctgacttcct ggcctggaaa attagtaaga gaagacgacg ttcacaattc
2821 atgtcaacaa agccccgagg aagggaaggt ggagcccgag aagttgaaga cactaacaga
2881 aggtttggaa gcctacagcc gtgtccggaa aaggaacaga aaaagtggaa agctaaataa
2941 ccatttagaa gctgctattc atgaggccat gagtgaactg gacaaaatgt ctgggactgt
3001 acaccaaatc ccacagggtg acagacaaat gagacccccc aaacccaaga ggaggaagat
3061 ctccagatgt acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta
3121 caaggatgac gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa
3181 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
3241 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
3301 tcctggttag ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
3361 ggggctcggc tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct
3421 attgctttat ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt
3481 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
3541 tcaggttcag ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg
3601 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
3661 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
3721 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
3781 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
3841 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
3901 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
3961 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4021 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
4081 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4141 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
4201 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4261 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
4321 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
4381 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
4441 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4501 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
4561 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
4621 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
4681 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
4741 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
4801 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
4861 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
4921 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
4981 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
5041 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5101 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5161 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5221 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5281 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5341 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5401 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5461 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
5521 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
5581 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
5641 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
5701 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
5761 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
5821 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
5881 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
5941 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
6001 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6061 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6121 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6181 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6241 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6301 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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