| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001427 |
| 产品用途: | 3' AAVLINK plasmid for KIAA0232 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KIAA0232 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG 6541 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6541)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6541
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3264
/codon_start=1
/label=KIAA0232
/translation="GLLKTFGQRWQTQILWLQ*K*KELMLSCFRQM*ITTAAV*MLKLN
WRPFRSLIRLCGGQSTICGRDRKRAWRKEHLLLVSYQTWMVVIIQHPLNPGM*PKIKKT
HSFLEEFMENSKPSIVMGSGQSYHLVTQKEVCYSVQLLML*R*LVQMSL*PQETVLLLG
TGSYGNPSCHLNRMISRRVGKMG*IRDFLLSSMKTY*ELVATFKSKILDLNTHFLPLT*
AIHFHKFFM*NAHLNLKGLHLSAPVLNRNQSSVLIQTVKCFTPGYVVLTEHNTGLFGSL
LGLTFAQFLHPNCPQEEEASQNLNLRKMKQIFPFLLKLIYLKIRRQISNLWKKMQRKKA
ITMENQSLSLENSFPG*KNLGWKRVLRHHWIPRRNQLGFFQ*ESKISVWNVA*MNPWK*
I*KAQKQIVK*WHNARKKLIIFVVAKQVVSFLLMKIIQFLRDSWKSSLY*TLIYKE*IE
VKKNRPGGKKPCTLLFFLHQSVKNVTQMPRERVV*KNIQMLKRHPVMKSAC*ILIGCLL
FMKQDVQEREILEQSQMASAERCAPAPAPPRKRQAQKAEASGWALVKRSSFLELI"
misc_feature 2433..2438
/label=SDS
misc_feature 2436..2441
/label=SDS
misc_feature 2663..2668
/label=SDS
CDS 3271..3336
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3352..3783
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3496..3501
/label=SDS
repeat_region 3826..3944
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3826..3933
/label=AAV2 ITR
rep_origin 4019..4474
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4756..4860
/gene="bla"
/label=AmpR promoter
CDS 4861..5721
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5892..6480
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggt tattaaagac
1561 atttggacaa agatggcaga cacaaattct gtggctacag tagaaataga aagaactgat
1621 gctgagttgt tttcggcaga tgtaaataac tactgctgct gtctagatgc tgaagctgaa
1681 ctggagaccc ttcaggagcc tgataaggct gtgcggaggt cagagtacca tctgtgggag
1741 ggacagaaag agagcctgga gaaaagagca tttgcttcta gtgagctatc aaacgtggat
1801 ggtggtgatt atacaacacc ctctaaaccc tgggatgtag cccaagataa agaaaacaca
1861 ttcattcttg gaggagttta tggagaactc aaaaccttca atagtgatgg ggagtgggca
1921 gtcgtaccac ctagtcacac aaaaggaagt ctgttacagt gtgcagcttc tgatgttgtg
1981 acgatagctg gtacagatgt ctttatgacc ccaggaaaca gttttgctcc tgggcacagg
2041 cagttatgga aacccttcgt gtcatttgaa cagaatgatc agccgaagag tggggaaaat
2101 gggttaaata agggattttc ttttatcttc catgaagact tactaggagc ttgtggcaac
2161 tttcaagtcg aagatcctgg acttgaatac tcattttctt cctttgactt aagcaatcca
2221 ttttcacaag ttcttcatgt agaatgctca tttgaacctg aagggattgc atctttcagc
2281 cccagtttta aaccgaaatc aatcctctgt tctgattcag acagtgaagt gtttcacccc
2341 aggatatgtg gtgttgacag aacacaatac agggctattc ggatctctcc tcggactcac
2401 tttcgcccaa tttctgcatc cgaactgtcc ccaggaggag gaagcgagtc agaatttgaa
2461 tctgagaaag atgaagcaaa tattcccatt ccttctcaag ttgatatatt tgaagatccg
2521 caggcagatc tcaaaccttt ggaagaagat gcagagaaag aaggccatta ctatggaaaa
2581 tcagagcttg agtctggaaa attccttccc aggttaaaaa aatctgggat ggaaaagagt
2641 gctcagacat cactggattc ccaggaggaa tcaactggga ttctttcagt aggaaagcaa
2701 aatcagtgtt tggaatgtag catgaatgaa tccctggaaa tagatttaga aagctcagaa
2761 gcaaattgta aaataatggc acaatgcgag gaagaaatta ataatttttg tggttgcaaa
2821 gcaggttgtc agtttcctgc ttatgaagat aatccagttt cttcgggaca gctggaagag
2881 ttccctgtat tgaacactga tatacaagga atgaatagaa gtcaagaaaa acagacctgg
2941 tgggaaaaag ccttgtactc tcctcttttt cctgcatcag agtgtgaaga atgttacaca
3001 aatgccaagg gagagagtgg tttagaagaa tatccagatg ctaaagagac acccagtaat
3061 gaagagcgcc tgttagattt taatagggtg tcttctgttt atgaagcaag atgtacagga
3121 gagagagatt ctggagcaaa gtcagatggc ttccgcggaa agatgtgctc cagcgccagc
3181 tccacctcgg aagagacagg ctcagaaggc ggaggcgagt gggtgggccc tagtgaagag
3241 gagctctttt ctcgaactca tctctgtaca gactacaaag accatgacgg tgattataaa
3301 gatcatgaca tcgactacaa ggatgacgat gacaagtaag aattctgatg actcgagata
3361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
3421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
3481 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
3541 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
3601 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
3661 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
3721 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
3781 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
3841 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
3901 tcgcccggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta
3961 ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac
4021 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
4081 acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
4141 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
4201 gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca
4261 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
4321 ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa
4381 gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac
4441 gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc
4501 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
4561 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
4621 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
4681 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
4741 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
4801 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
4861 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
4921 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
4981 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
5041 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
5101 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
5161 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
5221 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
5281 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
5341 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
5401 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
5461 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
5521 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
5581 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
5641 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
5701 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
5761 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
5821 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
5881 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
5941 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
6001 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
6061 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
6121 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
6181 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
6241 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
6301 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
6361 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
6421 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
6481 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
6541 t
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KIAA0232-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG
- 下一篇：没有了
