| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001373 |
| 产品用途: | 5' AAVLINK plasmid for KDM5C expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5C |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5C-FLAG 6694 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6694)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6694
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3395
/codon_start=1
/label=KDM5C
/translation="GHLGCHARTVGRGCQCSP*PCCG*SPGRAAGTADHC*TLGGESPP
LPGGQAEASTSHT*GHNP*SGKHPCSPAQHPGSQGGSC*GPGLDC*C**DPKW*PLPLP
G*LGGPSSCGPGPTCGAGGAETARATGTDSALLEGEGLQDLPQEKFLLHAAGGSLPMCR
CRLRQHQAQPVDGEGAGVVQI*HRAAGAVCAGPQGPRLCDRGLQGGGTEGEGGYPAAAS
HQFGQAQSTGIIEHGLLYNLYLCVWAGAGWGGSSAV*PVSGLVPWAVCVSASPPQLSEA
QSHLIPTAGLVGMGHQIPVSTVYALKAPAPGDHPGTAGSPAETACAAARGRGPAVPHRE
GHQLARPRQAGSGL*RCDCSFGTAG*APPTATG*T*T*GAS*LPCSPCF*PPQRGQWQG
YA*GPGLTGEWRQCDQS*EGSPGGGLR*ERSGAAVLAVATVDWPCVGTA*GNPGPLGGA
HDGGGPARGDPG*EPQHMAAAAGWTAPRPGEDPHTSGAGEGRASRESGSGPGPGEAAAA
EGGSGWGGR*PSPRGARAKEGTELRARG*GGPGGGRAGGGDWG*GPPCTHPHHWQPQHP
GEPEWLGTGGRDHFRPLGPFLHSDSPAASALPTAAASATV"
misc_feature 1783..1788
/label=SDS
misc_feature 2167..2172
/label=SDS
misc_feature 2188..2193
/label=SDS
misc_feature 2257..2262
/label=SDS
misc_feature 2369..2374
/label=SDS
misc_feature 2685..2690
/label=SDS
misc_feature 2812..2817
/label=SDS
misc_feature 3184..3189
/label=SDS
misc_feature 3193..3198
/label=SDS
misc_feature 3196..3201
/label=SDS
misc_feature 3211..3216
/label=SDS
CDS 3402..3467
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3483..3914
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3627..3632
/label=SDS
repeat_region 3957..4097
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3957..4086
/label=AAV2 ITR
rep_origin 4172..4627
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4909..5013
/gene="bla"
/label=AmpR promoter
CDS 5014..5874
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6045..6633
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggc accttggctg
1561 tcatgcgagg actgttggtc gcgggtgcca gtgtagcccc tagccctgct gtggataaag
1621 cccaggccga gctgcaggaa ctgctgacca ttgctgaacg ctgggaggag aaagcccacc
1681 tctgcctgga ggccaggcag aagcatccac cagccacact tgaggccata atccgtgaag
1741 cggaaaacat ccctgttcac ctgcccaaca tccaggctct caaggaggct cttgctaagg
1801 cccgggcctg gattgctgat gttgatgaga tccaaaatgg tgaccactac ccctgcctgg
1861 atgacttgga gggcctagta gctgtgggcc gggacctacc tgtggggctg gaggagctga
1921 gacagctaga gctacaggta ctgacagcgc actcctggag ggagaaggcc tccaagacct
1981 tcctcaagaa aaattcttgc tacacgctgc tggaggttct ctgcccatgt gcagatgccg
2041 gctcagacag caccaagcgc agccggtgga tggagaagga gctggggttg tacaaatctg
2101 acacagagct gctggggctg tctgcgcagg acctcaggga cccaggctct gtgatcgtgg
2161 ccttcaagga gggggaacag aaggagaagg agggtatcct gcagctgcgt cgcaccaatt
2221 cggccaagcc cagtccactg gcatcatcga gcacggcctc ctctacaacc tctatctgtg
2281 tgtgtgggca ggtgctggct ggggcgggag ctctgcagtg tgacctgtgt caggactggt
2341 tccatgggcg gtgtgtgtca gtgcctcgcc tcctcagctc tccgaggccc aatcccacct
2401 catccccact gctggcctgg tgggaatggg acaccaaatt cctgtgtcca ctgtgtatgc
2461 gctcaaggcg cccgcgcctg gagaccatcc tggcactgct ggtagccctg cagagactgc
2521 ctgtgcggct gcccgagggc gaggccctgc agtgcctcac agagagggcc atcagctggc
2581 aaggccgcgc caggcaggct ctggcctctg aagatgtgac tgctcttttg ggacggctgg
2641 ctgagctccg ccaacggcta caggctgaac ctagacctga ggagcctcct aactaccctg
2701 cagcccctgc ttctgacccc ctcagagagg gcagtggcaa ggatatgcct aaggtccagg
2761 gcttactgga gaatggagac agtgtgacca gtcctgagaa ggtagccccg gaggagggct
2821 caggtaagag agatctggag ctgctgtcct cgctgttgcc acagttgact ggccctgtgt
2881 tggaactgcc tgaggcaacc cgggccccct tggaggagct catgatggag ggggacctgc
2941 tcgaggtgac cctggatgag aaccacagca tatggcagct gctgcaggct ggacagcccc
3001 cagacctgga gaggatccgc acacttctgg agctggagaa ggcagagcgt cacgggagtc
3061 gggctcgggg ccgggccctg gagaggcggc ggcggcggaa ggtggatcgg ggtggggagg
3121 gcgatgaccc agcccgagag gagctagagc caaagagggt acggagctca gggccagagg
3181 ctgaggaggt ccaggaggag gaagagctgg aggaggagac tgggggtgag ggcccccctg
3241 cacccatccc caccactggc agccccagca cccaggagaa ccagaatggc ttggaaccgg
3301 cggaagggac cacttcaggc ccctcggccc ctttctccac tctgactccc cggctgcatc
3361 tgccctgccc acagcagccg cctcagcaac agttgtgtac agactacaaa gaccatgacg
3421 gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa gaattctgat
3481 gactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
3541 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
3601 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
3661 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
3721 tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
3781 ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
3841 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt
3901 gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga
3961 acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg
4021 gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc
4081 gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
4141 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
4201 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct
4261 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4321 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4381 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4441 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4501 aactctatct cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg
4561 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4621 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
4681 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
4741 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
4801 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
4861 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
6181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
6661 ttcctggcct tttgctggcc ttttgctcac atgt
//
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