| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001370 |
| 产品用途: | 5' AAVLINK plasmid for KDM5B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG
0001370 (Barcode) 6013 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6013)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6013
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2726
/codon_start=1
/label=KDM5B
/translation="GNGSLAVSQTRQ*REIAVASPRCGYKNLPMSEGPSCPYDSM*TLQ
GCFPHQLCGGTQYFTGPANLALSPLSEVRETSIRENSAPARLPSAYPSSPS*GRCTSIY
D*KNRELAAQSPATAFVRES*ICARSSGLRTVI*QMASLSRTGVRHKQGISTSWHNIIF
FA**LGQQNLIFALPLLNWTKLYPPPWC*SRSE*TIDGSPAAPGIPS*NSGTLPDFTCK
AKPCSAD*PKLTSETQQ*EE*LLPREARWN*QS*EKTEETPGKRGPLQ*AVGTS*ENAD
PQKEENQTEPPQGHEQFQVRERA*L*ISSFC*NSFPALRHILFRTGRL*G*RCHLPSCE
LPAARRR*GGLGPV*WQLQSVVSSGLCWCLPRDGRERRLHLCALYCEGRTKPK"
misc_feature 2013..2018
/label=SDS
CDS 2733..2798
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2802..3233
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2946..2951
/label=SDS
repeat_region 3276..3416
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3276..3405
/label=AAV2 ITR
rep_origin 3491..3946
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4228..4332
/gene="bla"
/label=AmpR promoter
CDS 4333..5193
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5364..5952
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa atggaagcct
1561 tgcagtctct cagactcgcc aatgaaggga aattgctgtc gcctctccaa gatgtggata
1621 taaaaatctg cctatgtcag aaggccccag ctgcccctat gattcaatgt gaactctgca
1681 gggatgcttt ccacaccagt tgtgtggcgg tacccagtat ttcacagggc ctgcgaatct
1741 ggctttgtcc ccattgtcgg aggtcagaga aacctccatt agagaaaatt ctgcccctgc
1801 tcgcctccct tcagcgtatc cgagttcgcc ttcctgaggg agatgcactt cgatatatga
1861 ttgaaagaac cgtgaactgg cagcacagag cccagcaact gctttcgtca gggaatctta
1921 aatttgtgca agatcgagtg ggctcaggac tgttatatag cagatggcaa gcctcagcag
1981 gacaggtgtc agacacaaac aaggtatctc aacctcctgg cacaacatca ttttctttgc
2041 ctgatgactg ggacaacaga acctcatatt tgcactcccc cttctcaact ggacgaagtt
2101 gtatccccct ccatggtgtt agtccagaag tgaatgaact attgatggaa gcccagctgc
2161 tccaggtatc ccttcctgaa attcaggaac tttaccagac tttacttgca aagccaagcc
2221 ctgctcagca gactgaccga agctcaccag tgagacccag cagtgagaag aatgactgtt
2281 gccgagggaa gcgagatgga attaacagtc ttgagagaaa actgaagaga cgcctggaaa
2341 gagagggcct ctccagtgag cggtgggaac gagttaagaa aatgcggacc cccaaaaaga
2401 agaaaatcaa actgagccac cccaaggaca tgaacaattt caagttagag agagagcgta
2461 gctatgaatt agttcgttct gctgaaactc attccctgcc ctcagacaca tcctattccg
2521 aacaggaaga ctctgaggat gaagatgcca tctgcccagc tgtgagctgc ctgcagccag
2581 aaggagatga ggtggactgg gtccagtgtg atggcagctg caatcagtgg tttcatcagg
2641 tctgtgttgg tgtctcccca gagatggcag agaaagaaga ctacatctgt gtgcgctgta
2701 ctgtgaagga cgcaccaagc cgaaagtgta cagactacaa agaccatgac ggtgattata
2761 aagatcatga catcgactac aaggatgacg atgacaagta actcgagata atcaacctct
2821 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
2881 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
2941 tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca tcgccgcctg
3001 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgtttat
3061 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt tgtgaaattt
3121 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
3181 attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt taaagatctg
3241 gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga tggagttggc
3301 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
3361 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg
3421 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag
3481 caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
3541 agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc
3601 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
3661 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg tgatggttca
3721 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
3781 tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc gggctattct
3841 tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga gctgatttaa
3901 caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg gtgcactctc
3961 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
4021 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
4081 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
4141 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
4201 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
4261 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
4321 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
4381 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
4441 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
4501 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
4561 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
4621 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
4681 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
4741 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
4801 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
4861 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
4921 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
4981 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
5041 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
5101 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
5161 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
5221 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
5281 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
5341 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
5401 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
5461 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
5521 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
5581 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
5641 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
5701 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
5761 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
5821 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
5881 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
5941 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
6001 tttgctcaca tgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5B-FLAG载体序列质粒序列等相关资料下载
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