| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001529 |
| 产品用途: | 5' AAVLINK plasmid for KDM5A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM5A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM5A-FLAG 6699 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6699)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6699
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3400
/codon_start=1
/label=KDM5A
/translation="VLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGL
EETRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQ
CELCKDWFHNSCVPLPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQK
LPVRLPEGEALQCLTERAMSWQDRARQALATDELSSALAKLSVLSQRMVEQAAREKTEK
IISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSSSPRQTMDYDDEETDSDEDIRETY
GYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEHAYSSASKSCSQG
SSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQA
THPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKM
DKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVL
DIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMA
ENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS"
misc_feature 1595..1600
/label=SDS
misc_feature 2481..2486
/label=SDS
misc_feature 3344..3349
/label=SDS
CDS 3407..3472
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3488..3919
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3632..3637
/label=SDS
repeat_region 3962..4102
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3962..4091
/label=AAV2 ITR
rep_origin 4177..4632
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4914..5018
/gene="bla"
/label=AmpR promoter
CDS 5019..5879
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6050..6638
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgc tgagcccccg
1561 gaccgacatt ggtgtatatg ggagtggcaa aaataggagg aaaaaagtaa aagaactaat
1621 agaaaaagaa aaagaaaagg atctggacct ggagcctctg agtgatctgg aggaaggatt
1681 ggaggaaacc agagatacag ccatggtggt ggcagttttc aaagaacggg agcaaaaaga
1741 gattgaagcc atgcattctc tcagagcagc caacctagcc aagatgacaa tggtggaccg
1801 catagaagaa gtaaaatttt gcatttgccg caagacagcc agtgggttta tgctacagtg
1861 tgagctctgc aaagactggt tccataacag ctgtgttcct cttcctaaat caagttccca
1921 aaaaaaagga tccagctggc aagctaaaga agtaaaattc ctttgccctc tttgtatgcg
1981 gtctcgaagg cccaggctag agactattct gtcactcctg gtatcccttc agaagttgcc
2041 cgtacggttg cctgaaggag aggccctgca gtgtttgaca gaacgtgcta tgagttggca
2101 agatagagcg cggcaggctc tagccacaga tgaactatcc tctgccctgg ccaaactatc
2161 tgtgttgagc cagcgtatgg tggaacaggc ggctcgagaa aaaactgaaa agatcatcag
2221 tgcagaactc caaaaagcag ctgccaatcc agacttacag ggacacttac ctagtttcca
2281 gcagtctgct tttaaccggg tggtgagcag tgtgtcatct tctcctcgac aaacaatgga
2341 ctatgatgat gaagaaacag actctgatga agacattcga gagacatatg gctacgacat
2401 gaaggacaca gccagtgtga agtcctctag tagtcttgaa cccaatcttt tttgtgatga
2461 agagattccc atcaaatccg aggaggtggt gacccacatg tggacagcac cttcattttg
2521 tgcagagcat gcttattctt ctgcttctaa gagttgttct caaggttcta gcaccccaag
2581 gaaacaacct cggaagagcc ctttggtgcc ccgaagtttg gaacctccag tgctggagtt
2641 gtcacctgga gctaaggcac aactggaaga acttatgatg gttggagatc tcctggaagt
2701 atctctggac gagactcaac acatatggcg gattttgcag gccacacacc caccctctga
2761 agacagattc ttgcatatca tggaggatga cagcatggaa gagaaaccac taaaagtgaa
2821 aggaaaggac tcttcagaga agaaacggaa acggaagcta gaaaaggtag agcaactttt
2881 tggagaagga aaacagaagt ccaaggagtt aaagaaaatg gacaaaccta gaaagaagaa
2941 attaaaatta ggtgcagaca aatcaaagga gctgaataaa ctggccaaga aactagcaaa
3001 agaagaagag agaaagaaaa agaaggagaa ggctgctgca gccaaagttg aacttgtgaa
3061 agagagcact gaaaagaaaa gagagaaaaa ggtgctggac atcccctcaa agtatgactg
3121 gtcaggagca gaggagtctg atgatgagaa tgctgtgtgc gcagcacaga actgccaaag
3181 gccctgcaag gacaaggtag actgggtaca atgtgatggt ggctgtgatg agtggtttca
3241 tcaagtttgt gtgggtgtat ctccagaaat ggctgaaaat gaagattaca tctgtataaa
3301 ctgtgcaaag aagcaggggc cagttagccc aggtccagca ccacctcctt ccttcataat
3361 gagctacaaa ctaccaatgg aggatcttaa agagaccagt tgtacagact acaaagacca
3421 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaagaatt
3481 ctgatgactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
3541 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
3601 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
3661 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
3721 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
3781 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
3841 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
3901 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
3961 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
4021 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
4081 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
4141 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
4201 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
4261 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
4321 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
4381 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
4441 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
4501 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
4561 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
4621 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
4681 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
4741 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
4801 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
4861 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
4921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
5041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
5101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
5161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
5221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
5281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
5341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
5401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
5461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
5521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
5581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
5641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
5701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
5761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
5821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
5881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
5941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
6001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
6061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
6121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
6181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
6241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
6301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
6361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
6421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
6481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
6541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
6601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
6661 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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