| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001352 |
| 产品用途: | 3' AAVLINK plasmid for KDM2B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM2B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-KDM2B-FLAG 6563 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6563)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6563
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
/note="Splicing donor"
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3276
/codon_start=3
/gene="KDM2B"
/gene_synonym="CXXC2; Fbl10; FBXL10; JHDM1B; PCCX2"
/product="lysine-specific demethylase 2B isoform a"
/EC_number="1.14.11.27"
/label=KDM2B
/note="isoform a is encoded by transcript variant 1;jumonji
C domain-containing histone demethylase 1B; F-box and
leucine-rich repeat protein 10; JEMMA (Jumonji domain,
EMSY-interactor, methyltransferase motif) protein; F-box
protein FBL10; lysine (K)-specific demethylase 2B;
F-box/LRR-repeat protein 10; CXXC-type zinc finger protein
2; protein-containing CXXC domain 2; [Histone-H3]-lysine-36
demethylase 1B; jmjC domain-containing histone
demethylation protein 1B"
/db_xref="GI:54112382"
/db_xref="CCDS:CCDS41850.1"
/db_xref="GeneID:84678"
/db_xref="HGNC:HGNC:13610"
/db_xref="MIM:609078"
/protein_id="NP_115979.3"
/translation="QEPAKRRSECEEAPRRRSDEHSKKVPPDGLLRRKSDDVHLRKKRK
YEKPQELSGRKRASSLQTSPGSSSHLSPRPPLGSSLSPWWRSSLTYFQQQLKPGKEDKL
FRKKRRSWKNAEDRMALANKPLRRFKQEPEDELPEAPPKTRESDHSRSSSPTAGPSTEG
AEGPEEKKKVKMRRKRRLPNKELSRELSKELNHEIQRTENSLANENQQPIKSEPESEGE
EPKRPPGICERPHRFSKGLNGTPRELRHQLGPSLRSPPRVISRPPPSVSPPKCIQMERH
VIRPPPISPPPDSLPLDDGAAHVMHREVWMAVFSYLSHQDLCVCMRVCRTWNRWCCDKR
LWTRIDLNHCKSITPLMLSGIIRRQPVSLDLSWTNISKKQLSWLINRLPGLRDLVLSGC
SWIAVSALCSSSCPLLRTLDVQWVEGLKDAQMRDLLSPPTDNRPGQMDNRSKLRNIVEL
RLAGLDITDASLRLIIRHMPLLSKLHLSYCNHVTDQSINLLTAVGTTTRDSLTEINLSD
CNKVTDQCLSFFKRCGNICHIDLRYCKQVTKEGCEQFIAEMSVSVQFGQVEEKLLQKLS
"
misc_feature 1580..1585
/label=SDS
misc_feature 1748..1753
/label=SDS
CDS 3283..3348
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3352..3783
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3496..3501
/label=SDS
repeat_region 3826..3966
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3826..3955
/label=AAV2 ITR
rep_origin 4041..4496
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4778..4882
/gene="bla"
/label=AmpR promoter
CDS 4883..5743
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5914..6502
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca ggaacctgcc
1561 aagcggagga gtgagtgtga ggaggcgccc cggcgcaggt cggatgagca ctcgaagaag
1621 gtgccgccgg acggccttct gcgcagaaag tctgacgacg tgcacctgag gaagaagcgg
1681 aaatacgaga agccccagga gctgagtgga cgcaagcggg cctcatcgct tcaaacgtcc
1741 cccggttcct cctctcacct ctcgccgagg ccccctctag gcagcagcct cagcccctgg
1801 tggagatcca gtctcactta cttccagcag cagctcaaac ctggcaaaga agataagctt
1861 ttcaggaaaa agcggcggtc ctggaagaac gccgaggacc gcatggcgct ggccaacaag
1921 cccctccggc gcttcaagca ggaacccgag gacgaactgc ccgaggcgcc ccccaagacc
1981 agggagagcg accactcccg ctccagctcc cccaccgcgg gacccagcac cgaaggggcc
2041 gagggcccgg aggagaagaa gaaggtgaag atgcgccgga agcggcggct tcccaacaag
2101 gagctgagca gggagctgag caaggagctc aaccacgaga tccagaggac ggagaacagc
2161 ctggccaacg agaaccagca gcccatcaag tcggagcctg agagcgaggg cgaggagccc
2221 aagcggcccc cgggcatctg cgagcgtccc caccgcttca gcaaggggct caacggcacc
2281 ccccgggagc tgcggcacca gctggggccc agcctgcgca gcccgccccg tgtcatctcc
2341 cggcccccac cctccgtgtc cccgcccaag tgtatccaga tggagcgcca tgtgatccgg
2401 ccacccccca tcagcccccc gcctgactcg ctacccctgg acgatggggc agcccacgtc
2461 atgcacaggg aggtgtggat ggccgtcttc agctacctca gccaccaaga cctgtgtgtg
2521 tgcatgcggg tctgcaggac ctggaaccgc tggtgctgcg ataagcggtt gtggacccgc
2581 attgacctga accactgcaa gtctatcaca cccctgatgc tgagtggcat catccggcga
2641 cagcccgtct ccctcgacct cagctggacc aatatctcca agaagcagct gagctggctc
2701 atcaaccggc tgcctgggct ccgggacttg gtgctgtcag gctgctcatg gatcgcggtc
2761 tcggcccttt gcagctccag ttgtccgctg ctccggaccc tggatgtcca gtgggtggag
2821 ggactaaagg atgcccagat gcgggatctc ctgtccccgc ccacagacaa caggccaggt
2881 cagatggaca atcggagcaa gctccggaac atcgtggagc tgcgcctggc aggcctggac
2941 atcacagatg cctccctgcg gctcatcatc cgccacatgc ccctgctctc caagctccac
3001 ctcagttact gtaaccacgt caccgaccag tctatcaacc tgctcactgc tgttggcacc
3061 accacccgag actccttaac cgagatcaac ctgtctgact gcaataaggt cactgatcag
3121 tgcctgtcct tcttcaaacg ctgtggaaac atctgtcata ttgacctgag gtactgcaag
3181 caagtcacca aggaaggctg tgagcagttc atagccgaga tgtctgtgag tgtccagttt
3241 gggcaagtag aagaaaaact cctgcaaaaa ctgagttgta cagactacaa agaccatgac
3301 ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta actcgagata
3361 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
3421 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
3481 tggctttcat tttctcctcc ttgtataaat cctggttagt tcttgccacg gcggaactca
3541 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
3601 tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc tagctttatt
3661 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
3721 aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg ggaggttttt
3781 taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa cccctagtga
3841 tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg
3901 tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctgcc
3961 tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
4021 tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
4081 ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc ctttcgcttt
4141 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
4201 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgatttggg
4261 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
4321 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca actctatctc
4381 gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt taaaaaatga
4441 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caattttatg
4501 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
4561 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
4621 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
4681 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
4741 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
4801 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
4861 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
4921 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
4981 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
5041 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
5101 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
5161 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
5221 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
5281 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
5341 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
5401 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
5461 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
5521 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
5581 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
5641 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
5701 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
5761 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
5821 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
5881 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
5941 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
6001 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
6061 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
6121 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
6181 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
6241 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
6301 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
6361 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
6421 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
6481 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
6541 ttgctggcct tttgctcaca tgt
//
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