| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001358 |
| 产品用途: | 3' AAVLINK plasmid for IQSEC2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: |
IQSEC2
|
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQSEC2-FLAG 7132 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7132)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7132
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3845
/codon_start=1
/label=IQSEC2
/translation="GASGYRPVQADLPAGDKA*LGLASFQQ*CGPEAALPNRPQPLQQE
AREGYPVSDRAGLPVRHTGGSGSLHPGAERPQPADDRGIPREPAEAVQQRRVGLCGG*D
GLLLHGSG*CAPEVPVPYPGSG*GPESGATHRSLQPAVLCL*PSPRAPVPEPRHHLHPC
FCHHPPQYRHVQSQRQS*TKDETR*LHQEPERG*QW*RHPPRPPGGHLPAHPGA*TADQ
R*PCVPGAGCGAHDCWKETSPVSPSPSTGLLLPALRGARSKPPPEARVASAGGLPLQ*S
PCGHQNFPEEEDLGDVQFPSVFPPRGNAHAALPEFILPVWDQVAVCSTWWGAKSPHHLQ
CPQPPGPAALYIRPARVHCGGAGDGEIPCGVGAGEAERYDAA*RLTAWRGQGLSEWDDG
PQ*PGGHLRGRRWAQTGRTQQFPARPL*CREAGAA*QRGIAGQHHRRVCY*QSTPSPED
ATSAPTPSARGVQEPEARLQLLILPGLPIWKQAGQGALPDATTANRPGLCLLFICFFHA
PPPPPPPSWP*PRWPGGAA*WAVQAPGPACPVLPRTGPCPATLPPTPAALSSPTSPAAP
TLAPAGLHSTASRLSPTCGATSPLPRPWPSPRAPTLYLGPARQGTQTGGWRTPSVCSTW
PPPPAPAHIPTAPVQSCPPAPSSPQTGP*ALHLQPPPTDDASSRRGWGPWIPATRGLLL
PPPPPPVTIVTTLTHPTPPLLSTPPPTLPSHPALTPSTHLPPWPAARLWAPWHSQPPQC
KPQGQAKPDQHRG"
misc_feature 2048..2053
/label=SDS
misc_feature 2162..2167
/label=SDS
misc_feature 3070..3075
/label=SDS
CDS 3096..3122
/codon_start=1
/product="9xHis affinity tag"
/label=9xHis
/translation="HHHHHHHHH"
misc_feature 3685..3690
/label=SDS
CDS 3852..3917
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3921..4352
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4065..4070
/label=SDS
repeat_region 4395..4535
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4395..4524
/label=AAV2 ITR
rep_origin 4610..5065
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5347..5451
/gene="bla"
/label=AmpR promoter
CDS 5452..6312
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6483..7071
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggag cctccggcta
1561 ccggcctgtg caagcagact taccagcggg agacaaggca tagctgggac tcgccagctt
1621 tcaacaatga tgtggtccag aggcggcact accgaatcgg cctcaacctc ttcaacaaga
1681 agccagagaa gggtatccag tatctgatcg agcggggctt cctgtcagac acaccggtgg
1741 gagtggctca cttcatcctg gagcggaaag gcctcagccg gcagatgata ggggaattcc
1801 tagggaaccg gcagaagcag ttcaacagag acgtgttgga ctgtgtggtg gatgagatgg
1861 acttctcctc catggatctg gatgatgcgc tccggaagtt ccagtcccat atccgggttc
1921 agggtgaggc ccagaaagtg gagcgactca tcgaagcctt cagccagcgg tactgtgtct
1981 gtaacccagc cctcgtgcgc cagttccgga acccagacac catcttcatc cttgcttttg
2041 ccatcatcct cctcaatacc gacatgtaca gtcccagcgt caaagctgaa cgaaagatga
2101 aactagatga cttcatcaag aacctgagag gggttgacaa tggtgaagac atcccccgag
2161 acctcctggt gggcatctac cagcgcatcc aggggcgtga actgcggacc aacgatgacc
2221 atgtgtccca ggtgcaggct gtggagcgca tgattgttgg aaagaaacca gtcctgtctc
2281 tccctcaccg tcgactggtt tgctgctgcc agctctacga ggtgccagat ccaaaccgcc
2341 cccagaggct agggttgcat cagcgggagg tcttcctctt caatgatctc cttgtggtca
2401 ccaaaatttt ccagaagaag aagatcttgg tgacgtacag tttccgtcag tctttccccc
2461 tcgtggaaat gcacatgcag ctcttccaga attcatatta ccagtttggg atcaagttgc
2521 tgtctgcagt acctggtggg gagcgaaaag tcctcatcat cttcaatgcc cccagcctcc
2581 aggaccggct gcgctttaca tccgacctgc gcgagtccat tgcggaggtg caggagatgg
2641 agaaataccg tgtggagtcg gagctggaga agcagaaagg tatgatgcgg cctaacgcct
2701 cacagcctgg aggggccaag gactcagtga atgggacgat ggcccgcagt agcctggagg
2761 acacttacgg ggcaggcgat gggctcaaac ggggcgcact cagcagttcc ctgcgagacc
2821 tctctgatgc agggaagcgg gggcggcgta acagcgtggg atcgctggac agcaccatcg
2881 aagggtctgt tattagcagt ccacgccctc accagaggat gccacctccg cccccacccc
2941 ctccgccaga ggagtacaag agccagaggc ccgtctccaa ctcctcatcc ttcctgggct
3001 ccctatttgg aagcaagcgg ggcaaggggc ccttccagat gccaccaccg ccaacaggcc
3061 aggcctctgc ctcctcttca tctgcttctt ccacgcacca ccaccaccac caccaccatc
3121 atggccatag ccacggtggc ctgggggtgc tgcctgatgg gcagtccaag ctccaggccc
3181 tgcatgccca gtattgccaa ggaccgggcc ctgccccgcc accctacctc ccaccccagc
3241 agccctctct tcccccacct ccccagcagc ccccaccctt gccccagctg ggctccattc
3301 caccgcctcc cgcctcagcc ccacctgtgg ggccacatcg ccacttccac gcccatggcc
3361 cagtcccagg gccccaacac tataccttgg gccggccagg cagggcaccc agacgggggg
3421 ctggaggaca ccctcagttt gctccacatg gccgccaccc cctgcaccag cccacatccc
3481 cactgcccct gtacagtcct gccccccagc accctccagc ccacaaacag ggccctaagc
3541 acttcatctt cagccaccac ccacagatga tgccagcagc aggcgcggct gggggccctg
3601 gatcccggcc accagggggc tcctactccc acccccacca cccccagtca ccattgtcac
3661 cacactcacc catcccaccc cacccctcct atccacccct ccccccaccc tcccctcaca
3721 ccccgcactc accccttcca cccacctccc cccatggccc gctgcacgcc tctgggcccc
3781 ctggcacagc caaccccccc agtgcaaacc ccaaggccaa gccaagccgg atcagcaccg
3841 tggtctgtac agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca
3901 aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa tttgtgaaag
3961 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
4021 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
4081 ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
4141 ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat
4201 tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg
4261 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
4321 aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg
4381 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
4441 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
4501 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
4561 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
4621 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4681 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4741 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4801 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
4861 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4921 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
4981 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
5041 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
5101 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
5161 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
5221 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
5281 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
5341 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
5401 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
5461 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
5521 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
5581 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
5641 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
5701 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
5761 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
5821 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
5881 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
5941 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
6001 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
6061 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
6121 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
6181 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
6241 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
6301 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
6361 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
6421 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
6481 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
6541 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
6601 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6661 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
6721 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6781 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6841 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6901 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6961 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
7021 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
7081 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
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