| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001511 |
| 产品用途: | 3' AAVLINK plasmid for IQGAP3 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | IQGAP3 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-IQGAP3-FLAG 6674 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6674)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6674
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..224
/label=SCP1
CDS 236..1264
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1280..1369
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1294..1299
/label=SDS
misc_feature 1297..1302
/label=SDS
intron 1373..1413
/label=Splicing Donor
protein_bind 1420..1453
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1454..1545
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1546..3375
/codon_start=1
/label=IQGAP3
/translation="VEQPQDVVTGNPTVVRLVVRFYRNGRGQSALQEILGKVIQDVLED
KVLSVHTDPVHLYKNWINQTEAQTGQRSHLPYDVTPEQALSHPEVQRRLDIALRNLLAM
TDKFLLAITSSVDQIPYGMRYVAKVLKATLAEKFPDATDSEVYKVVGNLLYYRFLNPAV
VAPDAFDIVAMAAGGALAAPQRHALGAVAQLLQHAAAGKAFSGQSQHLRVLNDYLEETH
LKFRKFIHRACQVPEPEERFAVDEYSDMVAVAKPMVYITVGELVNTHRLLLEHQDCIAP
DHQDPLHELLEDLGELPTIPDLIGESIAADGHTDLSKLEVSLTLTNKFEGLEADADDSN
TRSLLLSTKQLLADIIQFHPGDTLKEILSLSASREQEAAHKQLMSRRQACTAQTPEPLR
RHRSLTAHSLLPLAEKQRRVLRNLRRLEALGLVSARNGYQGLVDELAKDIRNQHRHRHR
RKAELVKLQATLQGLSTKTTFYEEQGDYYSQYIRACLDHLAPDSKSSGKGKKQPSLHYT
AAQLLEKGVLVEIEDLPASHFRNVIFDITPGDEAGKFEVNAKFLGVDMERFQLHYQDLL
QLQYEGVAVMKLFNKAKVNVNLLIFLLNKKFLRK"
misc_feature 1845..1850
/label=SDS
misc_feature 2001..2006
/label=SDS
misc_feature 2769..2774
/label=SDS
misc_feature 3348..3353
/label=SDS
CDS 3382..3447
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3463..3894
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3607..3612
/label=SDS
repeat_region 3937..4077
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3937..4066
/label=AAV2 ITR
rep_origin 4152..4607
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4889..4993
/gene="bla"
/label=AmpR promoter
CDS 4994..5854
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6025..6613
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg tgggggcgcg ttcgtcctca
181 gtcgcgatcg aacactcgag ccgagcagac gtgcctacgg accgcgtacg ccaccatgtc
241 caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga
301 ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg agcatacctg
361 gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa
421 atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg
481 tctggcagta aaaaccatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc
541 cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa
601 agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt
661 cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc
721 atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt
781 taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac
841 gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga
901 gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt
961 cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga
1021 agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag
1081 atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc
1141 tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtaat
1201 gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg
1261 cgatggaggt ggatctggtc tgctgctgaa actcctcctc ctcggtggtg gttctggtcc
1321 tcgtccccgt ggtactcgtg gtaaaggtcg ccgtattcac tacaaaggat aagtaagtat
1381 caaggttaca agacaggttt aaggagacca ataactagta taacttcgta taggatactt
1441 tatagcaatt tatgaaactg ggcttgtcga gacagagaag actcttgcgt ttctgatagg
1501 cacctattgg tcttactgac atccactttg cctttctctc cacaggtgga gcagccccag
1561 gacgtggtga caggcaaccc aacagtggtg aggctggtgg tgagattcta ccgtaatggg
1621 cggggacaga gtgccctgca ggagattctg ggcaaggtta tccaggatgt gctagaagac
1681 aaagtgctca gcgtccacac agaccctgtc cacctctata agaactggat caaccagact
1741 gaggcccaga cagggcagcg cagccatctc ccatatgatg tcaccccgga gcaggccttg
1801 agccaccccg aggtccagag acgactggac atcgccctac gcaacctcct cgccatgact
1861 gataagttcc ttttagccat cacctcatct gtggaccaaa ttccgtatgg gatgcgatat
1921 gtggccaaag tcctgaaggc aactctggca gagaaattcc ctgacgccac agacagcgag
1981 gtctataagg tggtcgggaa cctcctgtac taccgcttcc tgaacccagc tgtggtggct
2041 cctgacgcct tcgacattgt ggccatggca gctggtggag ccctggctgc cccccagcgc
2101 catgccctgg gggctgtggc tcagctccta cagcacgctg cggctggcaa ggccttctct
2161 gggcagagcc agcacctacg ggtcctgaat gactatctgg aggaaacaca cctcaagttc
2221 aggaagttca tccatagagc ctgccaggtg ccagagccag aggagcgttt tgcagtggac
2281 gagtactcag acatggtggc tgtggccaaa cccatggtgt acatcaccgt gggggagctg
2341 gtcaacacgc acaggctgtt gctggagcac caggactgca ttgcccctga tcaccaagac
2401 cccctgcatg agctcctgga ggatcttggg gagctgccca ccatccctga ccttattggt
2461 gagagcatcg ctgcagatgg gcacacggac ctgagcaagc tagaagtgtc cctgacgctg
2521 accaacaagt ttgaaggact agaggcagat gctgatgact ccaacacccg tagcctgctt
2581 ctgagcacca agcagctgtt ggccgatatc atacagttcc atcctgggga caccctcaag
2641 gagatcctgt ccctctcggc ttccagagag caagaagcag cccacaagca gctgatgagc
2701 cgacgccagg cctgtacagc ccagacaccg gagccactgc gacgacaccg ctcactgaca
2761 gctcactccc tcctgccact ggcagagaag cagcggcgcg tcctgcggaa cctacgccga
2821 cttgaagccc tggggttggt cagcgccaga aatggctacc aggggctagt ggacgagctg
2881 gccaaggaca tccgcaacca gcacagacac aggcacaggc ggaaggcaga gctggtgaag
2941 ctgcaggcca cattacaggg cctgagcact aagaccacct tctatgagga gcagggtgac
3001 tactacagcc agtacatccg ggcctgcctg gaccacctgg cccccgactc caagagttct
3061 gggaagggga agaagcagcc ttctcttcat tacactgctg ctcagctcct ggaaaagggt
3121 gtcttggtgg aaattgaaga tcttcccgcc tctcacttca gaaacgtcat ctttgacatc
3181 acgccgggag atgaggcagg aaagtttgaa gtaaatgcca agttcctggg tgtggacatg
3241 gagcgatttc agcttcacta tcaggatctc ctgcagctcc agtatgaggg tgtggctgtc
3301 atgaaactct tcaacaaggc caaagtcaat gtcaaccttc tcatcttcct cctcaacaag
3361 aagtttttgc ggaagtgtac agactacaaa gaccatgacg gtgattataa agatcatgac
3421 atcgactaca aggatgacga tgacaagtaa gaattctgat gactcgagat aatcaacctc
3481 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
3541 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
3601 ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc atcgccgcct
3661 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttta
3721 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat ttgtgaaatt
3781 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
3841 aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagatct
3901 ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
3961 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
4021 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
4081 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
4141 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
4201 cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc
4261 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
4321 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
4381 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
4441 ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc
4501 ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta
4561 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
4621 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
4681 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
4741 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
4801 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
4861 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
4921 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
4981 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
5041 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
5101 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
5161 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
5221 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
5281 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
5341 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
5401 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
5461 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
5521 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
5581 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
5641 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
5701 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
5761 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
5821 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
5881 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
5941 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
6001 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
6061 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
6121 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
6181 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
6241 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
6301 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
6361 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
6421 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
6481 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
6541 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
6601 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
6661 ttttgctcac atgt
//
