| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001502 |
| 产品用途: | 3' AAVLINK plasmid for HECW2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | HECW2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-HECW2-FLAG 6569 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6569)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 13, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6569
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3268
/codon_start=1
/label=HECW2
/translation="GKAFFVDHNSRTTTFIDPRLPLQSSRPTSALVHRQHLTRQRSHSA
GEVGEDSRHAGPPVLPRPSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNIFEILQERQ
PDLTRNHSLREKIQFIRTEGTPGLVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYC
QSPRGSPVSSPQNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGPGKLKLII
RRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGEEGLDYSGPSREFFFLVSRELFNPYYG
LFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKALLR
ILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQITERELKPGGANIPV
TEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELELVIAGTAEI
DLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLR
GSNGPRRFCVEKWGKITALPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTFGLE"
misc_feature 2956..2961
/label=SDS
CDS 3277..3342
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3358..3789
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3502..3507
/label=SDS
repeat_region 3832..3972
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3832..3961
/label=AAV2 ITR
rep_origin 4047..4502
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4784..4888
/gene="bla"
/label=AmpR promoter
CDS 4889..5749
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5920..6508
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca aggcattctt
1561 tgtggaccac aactcccgca ccaccacttt cattgatccc cggctcccac tccagagcag
1621 tagacccaca agtgcgctgg ttcatcggca acacctgaca aggcaacgca gccacagtgc
1681 gggtgaggta ggagaagatt ctcgacatgc aggaccacca gttcttccca ggccatccag
1741 tacattcaat acagtcagta ggccacagta ccaggacatg gttccagtgg cttacaatga
1801 caagattgtt gcatttctac gtcaacccaa tatctttgaa attctacagg agcgtcaacc
1861 tgatcttacc aggaatcact cactcaggga gaagatccaa tttatccgaa ctgaagggac
1921 tccaggattg gtgcgccttt caagcgatgc agaccttgtt atgttgctga gcttatttga
1981 agaagagata atgtcatatg tgcctccaca tgccttactc caccccagct actgtcagtc
2041 cccacgtggc tctcccgtgt catctcctca gaactcgcca ggtacccagc gtgccaatgc
2101 ccgggctcca gccccttaca agcgggattt cgaagccaaa ctgaggaact tttacaggaa
2161 gttagagact aaaggatatg gacaaggccc agggaagtta aagttaatta tccgaagaga
2221 tcacttacta gaagatgctt ttaatcagat tatgggctac tccagaaaag acctgcagag
2281 aaataagcta tatgtcacct tcgttgggga ggaagggctg gattacagtg ggccttctag
2341 agagtttttc ttcctggtat ccagagaact ctttaaccca tattatggct tatttgaata
2401 ttcagccaat gacacataca cagtacaaat aagtcctatg tctgcttttg tagacaatca
2461 ccatgaatgg ttccgattca gtggtaggat ccttggtctt gcactaatac accagtattt
2521 gttggatgcc ttcttcacac ggccctttta taaggctctt ctcagaattc tatgtgacct
2581 gagtgaccta gaataccttg atgaagagtt ccatcagagc ctgcagtgga tgaaagacaa
2641 tgatatccat gacatcctag acctcacgtt cactgtgaac gaagaagtat ttgggcagat
2701 aactgaacga gaattaaagc cagggggtgc caatatccca gttacagaga agaacaagaa
2761 ggagtacatc gagaggatgg tgaagtggag gattgagagg ggtgttgtac agcaaacaga
2821 gagcttagtg cgtggcttct atgaggtggt ggatgccagg ctggtatctg tttttgatgc
2881 aagagaactg gaattggtca tcgcaggcac agctgaaata gacctaagtg attggagaaa
2941 caacacagaa tatagaggag gataccatga caatcatatt gtaattcggt ggttctgggc
3001 tgcagtggaa agattcaaca atgaacaacg actaaggttg ttacagtttg ttacaggcac
3061 atccagcatt ccctatgaag gatttgcttc actccgaggg agtaacggcc caagaagatt
3121 ctgtgtggag aaatggggga aaatcactgc tcttcccaga gcgcatacat gttttaaccg
3181 tctggatctg cctccctacc catccttttc catgctttat gaaaaactgt tgacagcagt
3241 tgaagaaacc agtacttttg gacttgagtg tacaaggact acaaagacca tgacggtgat
3301 tataaagatc atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc
3361 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
3421 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
3481 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
3541 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
3601 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
3661 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
3721 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
3781 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
3841 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
3901 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
3961 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
4021 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
4081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
4141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
4201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
4261 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
4321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
4381 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
4441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
4501 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
4561 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
4621 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
4681 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
4741 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
4801 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
4861 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
4921 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
4981 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
5041 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
5101 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
5161 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
5221 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
5281 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
5341 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
5401 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
5461 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
5521 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
5581 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
5641 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
5701 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
5761 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
5821 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
5881 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
5941 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
6001 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
6061 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
6121 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
6181 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
6241 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
6301 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
6361 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
6421 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
6481 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
6541 ggccttttgc tggccttttg ctcacatgt
//
