pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2C-FLAG              6921 bp DNA     circular SYN 14-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6921
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3622
                     /codon_start=1
                     /label=DIP2C
                     /translation="AIDSIHQVGVYCLALVPANTLPKTPLGGIHLSETKQLFLEGSLHP
                     CNVLMCPHTCVTNLPKPRQKQPEIGPASVMVGNLVSGKRIAQASGRDLGQIEDNDQARK
                     FLFLSEVLQWRAQTTPDHILYTLLNCRGAIANSLTCVQLHKRAEKIAVMLMERGHLQDG
                     DHVALVYPPGIDLIAAFYGCLYAGCVPITVRPPHPQNIATTLPTVKMIVEVSRSACLMT
                     TQLICKLLRSREAAAAVDVRTWPLILDTDDLPKKRPAQICKPCNPDTLAYLDFSVSTTG
                     MLAGVKMSHAATSAFCRSIKLQCELYPSREVAICLDPYCGLGFVLWCLCSVYSGHQSIL
                     IPPSELETNPALWLLAVSQYKVRDTFCSYSVMELCTKGLGSQTESLKARGLDLSRVRTC
                     VVVAEERPRIALTQSFSKLFKDLGLHPRAVSTSFGCRVNLAICLQGTSGPDPTTVYVDM
                     RALRHDRVRLVERGSPHSLPLMESGKILPGVRIIIANPETKGPLGDSHLGEIWVHSAHN
                     ASGYFTIYGDESLQSDHFNSRLSFGDTQTIWARTGYLGFLRRTELTDANGERHDALYVV
                     GALDEAMELRGMRYHPIDIETSVIRAHKSVTECAVFTWTNLLVVVVELDGSEQEALDLV
                     PLVTNVVLEEHYLIVGVVVVVDIGVIPINSRGEKQRMHLRDGFLADQLDPIYVAYNM"
     CDS             3629..3694
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3710..4141
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3854..3859
                     /label=SDS
     repeat_region   4184..4324
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4184..4313
                     /label=AAV2 ITR
     rep_origin      4399..4854
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5136..5240
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5241..6101
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6272..6860
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggcga ttgacagtat
     1561 acatcaagtt ggagtttatt gcctggcctt ggtgccagca aacaccctcc ccaaaacccc
     1621 gcttggtggg atccatttat cagaaacaaa acagcttttt ctggagggct ctctgcaccc
     1681 ctgcaatgtc ctaatgtgcc cccacacctg cgtcacaaac ttgcctaagc ctcgacagaa
     1741 gcagccagaa atcggccctg cctctgtgat ggtggggaac ctggtctctg ggaagagaat
     1801 cgcccaggcc agtggcagag acctgggtca gatcgaagat aacgaccagg cacgcaagtt
     1861 cctgttcctc tcagaggtct tgcagtggag agcacagacc accccggacc acatcctcta
     1921 cacgctgctc aactgtcggg gtgcgatagc gaactcgctg acctgcgtgc agctgcacaa
     1981 gagagctgag aagatcgccg tgatgctgat ggagaggggc caccttcagg acggcgacca
     2041 cgtggccttg gtctaccccc caggaataga cctgatagca gcgttttatg gttgcctgta
     2101 cgcaggctgt gtgccaataa ccgtccgtcc cccgcaccca cagaacatcg cgacgacgtt
     2161 gcctaccgtc aagatgattg tggaggtgag tcgctctgcc tgtctgatga cgacacagct
     2221 gatctgtaag ttgctgcggt ccagggaggc ggcggcggct gtggacgtca ggacgtggcc
     2281 cctcatcctg gacacagatg atttgccaaa gaagcggcct gcccagatct gcaaaccttg
     2341 caacccagac actcttgcat atctcgactt cagcgtgtcc acaactggga tgctagctgg
     2401 cgtaaagatg tctcacgcag ccaccagtgc cttctgccgt tccattaagc tgcagtgtga
     2461 actttacccc tctagagaag tggccatctg cctggaccct tactgtggac tgggatttgt
     2521 cctctggtgc ctctgcagtg tgtattctgg gcaccagtcc atcctgatcc cgccctctga
     2581 gctggaaacc aaccccgcct tgtggcttct tgccgtgagt cagtacaaag tccgagacac
     2641 gttttgctcc tactccgtga tggagctgtg caccaagggg ctgggctcgc aaacagagtc
     2701 cctcaaggcg cgagggctgg acttgtcccg agtgaggacc tgcgtggttg tggcggaaga
     2761 gaggcctcgg atcgcactca cacagtcgtt ctcaaagctg tttaaggacc tgggccttca
     2821 cccgcgggcc gtcagcacct cgttcggttg cagggtgaac ctggcgattt gcttgcaggg
     2881 aacctcagga cctgacccaa ccactgtcta cgtggacatg agagccctga gacacgacag
     2941 agtccgctta gtggaaagag gatcccctca tagtctgccc ctgatggaat cgggaaagat
     3001 acttccaggg gttcggatta taattgccaa cccagaaaca aaaggaccgc tgggggactc
     3061 acaccttgga gagatttggg ttcacagtgc ccacaatgcc agcggttatt tcactattta
     3121 cggagacgaa tccctccagt cagatcactt caactcaaga ctaagttttg gagacaccca
     3181 gaccatctgg gcacgcacag gctacttggg gttcctgcgg agaactgagc tcacagatgc
     3241 aaatggagag cgccatgatg ccctctacgt ggtaggggca ctggacgaag ccatggagct
     3301 gcggggcatg cggtaccacc caatcgacat tgagacctcg gtcatcagag cccataaaag
     3361 cgttacggaa tgtgctgtgt ttacctggac aaatttgttg gtggttgtgg ttgagctgga
     3421 tgggtcggaa caagaagcct tggacctggt tcccttggtg accaacgtgg tcctggagga
     3481 gcactacctg atcgtcggag tggtggtcgt ggtggacatc ggcgtcatcc ccatcaactc
     3541 ccgtggggag aagcagcgca tgcacctgcg agacgggttt ttggcagacc agctagaccc
     3601 catctatgtg gcctacaaca tgtgtacaga ctacaaagac catgacggtg attataaaga
     3661 tcatgacatc gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat
     3721 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3781 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3841 gctttcattt tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc
     3901 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     3961 gtgtttattt gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg
     4021 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     4081 caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta
     4141 aagatctgga gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg
     4201 gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc
     4261 gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg
     4321 caggggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     4381 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4441 ttacgcgcag cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct
     4501 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4561 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4621 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4681 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg
     4741 gctattcttt tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc
     4801 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
     4861 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     4921 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     4981 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     5041 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     5101 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5161 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5221 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5281 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5341 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     5401 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     5461 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     5521 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     5581 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     5641 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     5701 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     5761 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     5821 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     5881 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     5941 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6001 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6061 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6121 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6181 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6241 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6301 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6361 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     6421 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     6481 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     6541 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     6601 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     6661 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     6721 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     6781 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     6841 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     6901 gctggccttt tgctcacatg t
//