| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001379 |
| 产品用途: | 3' AAVLINK plasmid for DIP2A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DIP2A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-DIP2A-FLAG 6788 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6788)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6788
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3501
/codon_start=1
/label=DIP2A
/translation="GRCTRVMC*CALTPVLPTSPNLVRNNQRLDQPQ*SWGTWLLGRES
LRLPGESSPTWRTATRHGSSCSWLTCCSGVPTPLLTTRCSCC*TPRAPSQALQPVSSCT
KGLREWPRL*WRRED*VLGTMWLWSTHQGWTSLPRSMAACTVAACLSPCGPRTLRTSAP
HCPPSR*SWRSASLHASSPRRLSHGCSGPRRLLLPWTSGPGPPS*TQMTSQKRR*QAFS
GPPPPMSSHTWTSACQPLGY*RE*RCRTRPQAPYAAP*SCSVSCTPRGRSPSASTPTVA
LVLPCGVCAVSTRDTNQCWCPRWSWRATCPCGCRPSASTRPASPSAPTL*WRCAPRA*A
HRRVSSG*RG*TCHVCARAWWSPRSGPGLR*PSPSPSSSRTWACRPAP*APRSGAGSTW
PSASRAQLARTPQPSTWTCGHCAMTGFVW*NGVLRTACH*WSLERSSPA*RSSSHTPRP
KDPWETHTWERSG*AAPTMPPGTTPFTGRRRFMPTTSVPG*VLETHRPSGQGPATLASF
GEQSSLMPVEGGTMHCMWLGLWMKLWSSEACGTTPSTLRPLSSEHTGASLSVPYSPGPT
CWWWWWSWMG*SRMPWTWWPW*PTWCWRSTTWSWEWWSSWTQG*SLSTLGVRSSACTCG
TASWLTSWTPSMSPTT"
misc_feature 2123..2128
/label=SDS
misc_feature 3005..3010
/label=SDS
CDS 3508..3573
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3577..4008
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3721..3726
/label=SDS
repeat_region 4051..4191
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4051..4180
/label=AAV2 ITR
rep_origin 4266..4721
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5003..5107
/gene="bla"
/label=AmpR promoter
CDS 5108..5968
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6139..6727
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggac gctgcacccg
1561 tgtaatgtgc tgatgtgccc tcacacctgt gttaccaacc tccccaaacc tcgtcagaaa
1621 caaccagagg ttggaccagc ctcaatgatc gtggggaacc tggttgctgg gaagagaatc
1681 gctcaggctt ccgggagaga gctcgcccac ctggaggaca gcgaccaggc acggaagttc
1741 ctgttcctgg ctgacgtgct gcagtggcgt gcccacacca ctcctgacca cccgctgttc
1801 ttgctgctga acgccaaggg caccgtcaca agcactgcaa cctgtgtcca gctgcacaaa
1861 agggctgaga gagtggccgc ggctctgatg gagaagggaa gactgagtgt tggggaccat
1921 gtggctctgg tctacccacc aggggtggac ctcattgccg cgttctatgg ctgcttgtac
1981 tgtggctgcg tgcctgtcac cgtgcggccc ccgcaccctc agaacctcgg caccacactg
2041 cccaccgtca agatgatcgt ggaggtcagc aagtctgcat gcgtcctcac cacgcaggct
2101 gtcacacggc tgctcaggtc caaggaggct gctgctgccg tggacatcag gacctggccc
2161 accatcctag acacagatga catcccaaaa aagaagatag caagcgtttt caggcccccc
2221 tcccccgatg tcctcgcata cttggacttc agcgtgtcaa ccactgggat attagcggga
2281 gtgaagatgt cgcacgcggc cacaagcgcc ttatgccgct ccataaagct gcagtgtgag
2341 ctgtacccct cgcggcagat cgccatctgc ctcgacccct actgtggcct tggttttgcc
2401 ctgtggtgtc tgtgcagtgt ctactcggga caccaatcag tgctggtgcc cccgctggag
2461 ctggagagca acgtgtccct gtggctgtcg gccgtcagcc agtacaaggc ccgcgtcacc
2521 ttctgctcct actctgtgat ggagatgtgc accaagggcc taggcgcaca gacgggtgtc
2581 ctcaggatga agggggtgaa cctgtcatgt gtgcgcacgt gcatggtggt cgccgaggag
2641 cggcccagga ttgcgctgac ccagtccttc tccaagctct tcaaggacct gggcctgccg
2701 gcccgcgccg taagcaccac gttcgggtgc agggtcaacg tggccatctg cctccagggc
2761 acagctggcc cggaccccac aaccgtctac gtggacatgc gggcactgcg ccatgacagg
2821 gttcgtttgg tagaacgggg ttctccgcac agcctgccat tgatggagtc tggaaagatc
2881 ctccccggcg tgaaggtcat catcgcacac accgagacca aaggaccctt gggagactca
2941 cacctgggag agatctgggt aagcagcccc cacaatgcca ccgggtacta caccgtttac
3001 ggggaggagg cgcttcatgc cgaccacttc agtgcccggc tgagttttgg agacacacag
3061 accatctggg caaggaccgg ctaccttggc ttccttcggc gaacagagct cactgatgcc
3121 agtggagggc ggcacgatgc actgtatgtg gttgggtctc tggatgaaac tctggagctc
3181 agaggcatgc ggtaccaccc catcgacatt gagacctctg tcatccgagc acacaggagc
3241 atcgctgagt gtgccgtatt cacctggacc aacctgctgg tggtggtggt ggagctggat
3301 gggctagagc aggatgccct ggacctggtg gccctggtga ccaacgtggt gctggaggag
3361 cactacctgg tcgtgggagt ggtggtcatc gtggacccag gggtgatccc tatcaactct
3421 cggggtgaga agcagcgcat gcacctgcgg gacggcttcc tggctgacca gctggacccc
3481 atctatgtcg cctacaacat gtgtacagac tacaaagacc atgacggtga ttataaagat
3541 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
3601 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
3661 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
3721 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
3781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
3841 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
3901 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
3961 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag atctggagac
4021 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
4081 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
4141 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
4201 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
4261 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
4321 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
4381 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
4441 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
4501 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
4561 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
4621 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
4681 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
4741 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
4801 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
4861 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
4921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
4981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
5041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
5101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
5161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
5221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
5281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
5341 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
5401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
5461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
5521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
5581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
5641 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
5701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
5761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
5821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
5881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
5941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
6001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
6061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
6121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
6181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
6241 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
6301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
6361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
6421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
6481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
6541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
6601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
6661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
6721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
6781 tcacatgt
//
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