| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001541 |
| 产品用途: | 3' AAVLINK plasmid for CHD1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CHD1-FLAG 7112 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7112)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7112
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3825
/codon_start=1
/label=CHD1
/translation="VLPHQVLLLSIKKSYQPF*SLVLKNFLRNLKEKNKSPRKWI*MKS
*RELKLMKMNQVL*L*EMNCFPSSRLPTSQIWMRMTLSWNLKEIQRIGRKLFQKIKEDD
*KKKKDKRNLKKFICSQE*EIVQNRLVSMEVKGGAVEVGDTLDLIVIPSQKGKGQRNVE
DHGLFLGRILKDLVMQKLGGLSRAIRNLVVLWKD*MQLLEMLS*LISQKQTLDDWENWY
IMVALKH*RIVLQEQNEQVVDSEK*RVQHSEYQEYR*MPN*SSPMKKN*YLCTNPFLLI
QKKESSILSHATQRQLILI*TGAKKMIPIC*LASMNMDMEAGK*LKWILTSV*HTRFFQ
MIPIKNHKQNSCRPVQTTSSNYLVEILQKKKLFLVREVQRGEKQELRRIKQ*SL*K*KR
K*RVILLLCLQRSLMKMMIN*VNPSLMVGKDPRNLQCQMLQFISRQVVNQFPFLKNLKS
WIRRHSAFVKKE*GLLKQL*NNLIGLRKAFQKENN*SILDNV**KLETISQNV*KSIQI
LNKLSNGEKTCGFLYLSLLNLMQENYINYISMLLKNGRSLSKTVIKTAT*ILT*LEIQM
WKD*KRIQITMIAAGTVIPLIDT*LSTMIIIKTDIREILTKKVIPGKDPILLLVMVKTI
VIGITTSKTADITVTERNTENWMITGVEITGQIWKEV*KIDLILIIVLTQIIGYIQTTG
QVLNIRTINLPGIIGITQTGKWTTELPAVALGHH*IRDLLMAPDLHLNIQLNTKVHRSI
PGVVGK"
protein_bind 1777..1788
/label=ZF binding site
/bound_moiety="CCR5TC zinc-finger domain"
/note="target sequence for the CCR5TC zinc-finger domain
from the CCR5-224 (+) zinc-finger nuclease (Perez et al.,
2008; Gross et al., 2013)"
RBS 2682..2693
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 3832..3897
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3901..4332
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4045..4050
/label=SDS
repeat_region 4375..4515
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4375..4504
/label=AAV2 ITR
rep_origin 4590..5045
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5327..5431
/gene="bla"
/label=AmpR promoter
CDS 5432..6292
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6463..7051
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggttc tgccccatca
1561 agttctactc ctttcaataa agaagagtta tcagccattt taaagtttgg tgctgaagaa
1621 ctttttaagg aacctgaagg agaagaacaa gagccccagg aaatggatat agatgaaatc
1681 ttgaagagag ctgaaactca tgaaaatgaa ccaggtcctt taactgtagg agatgaattg
1741 ctttcccagt tcaaggttgc caacttctca aatatggatg aggatgacat tgagttggaa
1801 cctgaaagaa attcaaagaa ttgggaggaa attattccag aagatcaaag aagacgatta
1861 gaagaagaag aaagacaaaa ggaacttgaa gaaatttata tgctcccaag aatgagaaat
1921 tgtgcaaaac agattagttt caatggaagt gaagggaggc gcagtagaag taggagatac
1981 tctggatctg atagtgattc catctcagaa gggaaaaggc caaagaaacg tggaagacca
2041 cggactattc ctcgggagaa tattaaagga tttagtgatg cagaaattag gcggtttatc
2101 aagagctata agaaatttgg tggtcctctg gaaagattag atgcaattgc tcgagatgct
2161 gagttagttg ataagtcaga aacagacctt agacgactgg gagaattggt acataatggt
2221 tgcattaaag cattaaagga tagttcttca ggaacagaac gaacaggtgg tagactcgga
2281 aaagtgaagg gtccaacatt ccgaatatca ggagtacagg tgaatgccaa actagtcatc
2341 tcccatgaag aagaattaat acctttgcac aaatccattc cttctgatcc agaagaaaga
2401 aagcagtata ctatcccatg ccacacaaag gcagctcatt ttgatataga ctggggcaaa
2461 gaagatgatt ccaatttgtt aattggcatc tatgaatatg gatatggaag ctgggaaatg
2521 attaaaatgg atcctgacct cagtctaaca cacaagattc ttccagatga tcccgataaa
2581 aaaccacaag caaaacagtt gcagacccgt gcagactacc tcatcaaatt acttagtaga
2641 gatcttgcaa aaaaagaagc tctttctggt gcgggaagtt caaagaggag aaaagcaaga
2701 gctaagaaga ataaagcaat gaagtctata aaagtgaaag aggaaataaa gagtgattct
2761 tctcctctgc cttcagagaa gtctgatgaa gatgatgata aattgagtga atccaagtct
2821 gatggtaggg aaagatccaa gaaatcttca gtgtcagatg ctccagttca tatcacggca
2881 agtggtgaac cagttcccat ttctgaagaa tctgaagagc tggatcagaa gacattcagc
2941 atttgtaaag aaagaatgag gcctgttaaa gcagctttga aacaacttga taggcctgag
3001 aaaggccttt cagaaagaga acaactagag catactagac aatgtttaat aaaaattgga
3061 gaccatatca cagaatgtct aaaagagtat acaaatcctg aacaaattaa gcaatggaga
3121 aaaaacctgt ggatttttgt atctaagttt actgaatttg atgcaagaaa attacataaa
3181 ttatataagc atgctattaa aaaacggcag gagtctcagc aaaacagtga tcaaaacagc
3241 aacttgaatc ctcacgtgat tagaaatcca gatgtggaaa gattaaaaga gaatacaaat
3301 cacgatgata gcagcaggga cagttattcc tctgatagac acttaactca gtaccatgat
3361 catcataaag accgacatca gggagattct tacaaaaaaa gtgattccag gaaaagaccc
3421 tattcttctt ttagtaatgg taaagaccat cgtgattggg atcactacaa gcaagacagc
3481 agatattaca gtgacagaga gaaacacaga aaactggatg atcacaggag tagagatcac
3541 aggtcaaatt tggaaggaag tttaaaagat agatctcatt ctgatcatcg ttctcactca
3601 gatcatcggt tacattcaga ccaccggtca agttctgaat atacgcacca taaatcttcc
3661 agggattata ggtatcactc agactggcaa atggaccaca gagcttccag cagtggccct
3721 aggtcaccac tagatcagag atctccttat ggctccagat ctccatttga acattcagtt
3781 gaacacaaaa gtacaccgga gcatacctgg agtagtcgga aaacatgtac agactacaaa
3841 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa
3901 ctcgagataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
3961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
4021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttagtt cttgccacgg
4081 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
4141 acaattccgt ggtgtttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatct
4201 agctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
4261 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg
4321 gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg gaccgagcgg ccgcaggaac
4381 ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc
4441 gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc
4501 gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
4561 cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc
4621 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc
4681 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
4741 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
4801 tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
4861 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
4921 ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt
4981 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac
5041 aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
5101 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
5161 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
5221 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
5281 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
5341 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
5401 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
5461 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
5521 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
5581 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
5641 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
5701 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
5761 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
5821 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
5881 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
5941 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
6001 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
6061 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
6121 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
6181 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
6241 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
6301 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
6361 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
6421 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
6481 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
6541 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
6601 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
6661 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
6721 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
6781 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
6841 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
6901 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
6961 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
7021 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
7081 cctggccttt tgctggcctt ttgctcacat gt
//
