| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001481 |
| 产品用途: | 3' AAVLINK plasmid for CECR2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CECR2 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-CECR2-FLAG 6725 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6725)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6725
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3426
/codon_start=1
/label=CECR2
/translation="GTCDRPASLPDIGYSHLQCQHPVLCLEHLPRLFGGCREGTP*WTA
QR*LRCSSSPPASAPQVCLTTPTSLHTPVYLALFRR*LTQCQSLCQPPSLPWATLGGHR
RTVKHKSLRMTKQSRCLALKRNHQVLVLQRGSTSHNYLTPHLPCRLTAPGRAHHKKGKQ
WARSSKAAPPNLRTTVKQ*RARIPGPRIAATPAQPPKGA*ETSPRWQTGALYPRTESLG
KHLLVDRRGRALVAVVPKSCSAPEAERCRKPCHARDRTQRHRPAQTPV*REAL*ASFPR
CICLA*STRIQLPITTSVQACRVWAL*WEGSPQHPIPSIFPQGAFSLTTHILEAFPGIA
PHKE*GIPTTHRHSLPTTTISELLTMPVHRAFLTGRDLSIPREAQADPQPVSLPHQGPS
SQIRMPWPVCKAVRH*MLP*LLQPVWMQWLLKSQMTGRILVQRKRSWMNLWRGQRVPKN
F*TWTTITQLPSGRARCQPASISMELLRL*VQEWDLVHLHFHPTV*CCRRGLPIPLSGR
PVTFSPGLTLPLWLPSHLTTQGPPSPTASLRRVPSIAARKKAWVTFKL**WNKLALEVE
*EDLSRKCTDHQECRCTRSSRRPRSQRPPQQQHHRRRCRLISLQHFPWIR"
misc_feature 3188..3193
/label=SDS
misc_feature 3377..3382
/label=SDS
misc_feature 3380..3385
/label=SDS
CDS 3433..3498
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3514..3945
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3658..3663
/label=SDS
repeat_region 3988..4128
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3988..4117
/label=AAV2 ITR
rep_origin 4203..4658
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4940..5044
/gene="bla"
/label=AmpR promoter
CDS 5045..5905
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6076..6664
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggta catgcgaccg
1561 ccctgcaagt ctgccggaca tcggttacag ccacctccag tgccagcacc cagttctttg
1621 tttggagcac ctgcccaggc tcttcggggg gtgcagggag gggactccat gatggacagc
1681 ccagagatga ttgcgatgca gcagctctcc tcccgcgtct gccccccagg tgtgccttac
1741 cacccccacc agcctgcaca cccccgttta cctggccctt ttccgcaggt agctcaccca
1801 atgtcagtca ctgtgtcagc ccccaagcct gccctgggca accctgggag ggcaccggag
1861 aacagtgaag cacaagagcc tgagaatgac caagcagagc cgttgcctgg ccttgaagag
1921 aaaccaccag gtgttggtac ttcagagggg gtctacctca cacaactacc tcaccccaca
1981 cctcccctgc agactgactg caccaggcag agctcaccac aagaaaggga aacagtgggc
2041 ccggagctca aaagcagctc ctccgaatct gcggacaact gtaaagcaat gaagggcaag
2101 aatccctggc cctcggatag cagctacccc ggcccagccg cccaagggtg cgtgagagac
2161 ctctccacgg tggcagacag gggcgctcta tccgagaacg gagtcattgg ggaagcatct
2221 ccttgtggat cggaggggaa gggccttggt agcagtggtt ccgaaaagct gctctgcccc
2281 agaggcagaa cgttgcagga aaccatgcca tgcacgggac agaacgcagc gacaccgccc
2341 agcacagacc ccggtttgac gggaggcact gtgagccagt ttcccccgct gtatatgcct
2401 ggcctagagt acccgaattc agctgcccat taccacatca gtccaggcct gcagggtgtg
2461 ggccctgtga tgggagggaa gtccccagca tcccatcccc agcattttcc cccaaggggc
2521 tttcagtcta accacccaca ttctggaggc tttccccggt atcgcccccc acaaggaatg
2581 aggtattcct accacccacc gccacagcct tcctaccacc actatcagcg aactccttac
2641 tatgcctgtc cacagagctt ttctgactgg cagagacctc tccatcccca gggaagccca
2701 agcggacccc cagccagtca gcctccccca ccaaggtccc tcttctcaga taagaatgcc
2761 atggccagtc tgcaaggctg tgagacactg aatgctgcct taacttctcc aacccgtatg
2821 gatgcagtgg ctgctaaagt cccaaatgac gggcagaatc ctggtccaga ggaagagaag
2881 ctggatgaat ctatggagag gccagagagt cccaaagaat ttttagacct ggacaaccat
2941 aacgcagcta ccaagcggca gagctcgttg tcagccagcg agtatctcta tggaactcct
3001 ccgcctctga gttcaggaat gggatttggt tcatctgcat ttccacccca cagtgtgatg
3061 ctgcagacgg ggcctcccta tacccctcag cggccggcca gtcactttca gcccagggct
3121 tactcttccc ctgtggctgc cctcccacct caccacccag gggccaccca gcccaacggc
3181 ctctctcagg agggtcccat ctatcgctgc caggaagaag gcctgggtca ctttcaagct
3241 gtgatgatgg aacaaattgg cactagaagt ggaataagag gacctttcca ggaaatgtac
3301 agaccatcag gaatgcagat gcacccggtc cagtcgcagg cctcgttccc aaagaccccc
3361 acagcagcaa catcacagga ggaggtgccg cctcataagc ctccaacact tcccctggat
3421 cagagctgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
3481 aaggatgacg atgacaagta agaattctga tgactcgaga taatcaacct ctggattaca
3541 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
3601 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
3661 ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc
3721 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa
3781 tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct
3841 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
3901 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg
3961 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
4021 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4081 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
4141 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
4201 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
4261 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
4321 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
4381 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
4441 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
4501 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
4561 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
4621 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
4681 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
4741 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
4801 ctgcatgtgt cagaggtttt caccgtcatc accggaacgc gcgagacgaa agggcctcgt
4861 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
4921 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
4981 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
5041 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
5101 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
5161 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
5221 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5281 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5341 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5401 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5461 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
5521 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
5581 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
5641 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
5701 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
5761 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
5821 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
5881 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
5941 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
6001 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6061 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6121 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6181 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6241 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6301 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6361 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6421 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6481 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
6541 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
6601 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
6661 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
6721 catgt
//
