| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001553 |
| 产品用途: | 3' AAVLINK plasmid for C4B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | C4B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-C4B-FLAG 6690 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6690)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6690
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3391
/codon_start=1
/label=C4B
/translation="GGLVGNDETVALTAFVTIALHHGLAVFQDEGAEPLKQRVEASISK
ANSFLGEKASAGLLGAHAAAITAYALTLTKAPADLRGVAHNNLMAMAQETGDNLYWGSV
TGSQSNAVSPTPAPRNPSDPMPQAPALWIETTAYALLHLLLHEGKAEMADQASAWLTRQ
GSFQGGFRSTQDTVIALDALSAYWIASHTTEERGLNVTLSSTGRNGFKSHALQLNNRQI
RGLEEELQFSLGSKINVKVGGNSKGTLKVLRTYNVLDMKNTTCQDLQIEVTVKGHVEYT
MEVNEDYEDYEYDELPAKDDPDAPLQPVTPLQLFEGRRNRRRREAPKVVEEQESRVHYT
VCIWRNGKVGLSGMAIADVTLLSGFHALRADLEKLTSLSDRYVSHFETEGPHVLLYFDS
VPTSRECVGFEAVQEVPVGLVQPASATLYDYYNPERRCSVFYGAPSKSRLLATLCSAEV
CQCAEGKCPRQRRALERGLQDEDGYRMKFACYYPRVEYGFQVKVLREDSRAAFRLFETK
ITQVLHFTKDVKAAANQMRNFLVRASCRLRLEPGKEYLIMGLDGATYDLEGHPQYLLDS
NSWIEEMPSERLCRSTRQRAACAQLNDFLQEYGTQGCQV"
misc_feature 1972..1977
/label=SDS
misc_feature 2223..2228
/label=SDS
misc_feature 2513..2518
/label=SDS
misc_feature 2626..2631
/label=SDS
misc_feature 3171..3176
/label=SDS
CDS 3398..3463
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3479..3910
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3623..3628
/label=SDS
repeat_region 3953..4093
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3953..4082
/label=AAV2 ITR
rep_origin 4168..4623
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4905..5009
/gene="bla"
/label=AmpR promoter
CDS 5010..5870
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6041..6629
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggggg gtttggtggg
1561 caatgatgag actgtggcac tcacagcctt tgtgaccatc gcccttcatc atgggctggc
1621 cgtcttccag gatgagggtg cagagccatt gaagcagaga gtggaagcct ccatctcaaa
1681 ggcaaactca tttttggggg agaaagcaag tgctgggctc ctgggtgccc acgcagctgc
1741 catcacggcc tatgccctga cactgaccaa ggcccctgcg gacctgcggg gtgttgccca
1801 caacaacctc atggcaatgg cccaggagac tggagataac ctgtactggg gctcagtcac
1861 tggttctcag agcaatgccg tgtcgcccac cccggctcct cgcaacccat ccgaccccat
1921 gccccaggcc ccagccctgt ggattgaaac cacagcctac gccctgctgc acctcctgct
1981 tcacgagggc aaagcagaga tggcagacca ggcttcggcc tggctcaccc gtcagggcag
2041 cttccaaggg ggattccgca gtacccaaga cacggtgatt gccctggatg ccctgtctgc
2101 ctactggatt gcctcccaca ccactgagga gaggggtctc aatgtgactc tcagctccac
2161 aggccggaat gggttcaagt cccacgcgct gcagctgaac aaccgccaga ttcgcggcct
2221 ggaggaggag ctgcagtttt ccttgggcag caagatcaat gtgaaggtgg gaggaaacag
2281 caaaggaacc ctgaaggtcc ttcgtaccta caatgtcctg gacatgaaga acacgacctg
2341 ccaggaccta cagatagaag tgacagtcaa aggccacgtc gagtacacga tggaagtaaa
2401 cgaggactat gaggactatg agtacgatga gcttccagcc aaggatgacc cagatgcccc
2461 tctgcagccc gtgacacccc tgcagctgtt tgagggtcgg aggaaccgcc gcaggaggga
2521 ggcgcccaag gtggtggagg agcaggagtc cagggtgcac tacaccgtgt gcatctggcg
2581 gaacggcaag gtggggctgt ctggcatggc catcgcggac gtcaccctcc tgagtggatt
2641 ccacgccctg cgtgctgacc tggagaagct gacctccctc tctgaccgtt acgtgagtca
2701 ctttgagacc gaggggcccc acgtcctgct gtattttgac tcggtcccca cctcccggga
2761 gtgcgtgggc tttgaggctg tgcaggaagt gccggtgggg ctggtgcagc cggccagcgc
2821 aaccctgtac gactactaca accccgagcg cagatgttct gtgttttacg gggcaccaag
2881 taagagcaga ctcttggcca ccttgtgttc tgctgaagtc tgccagtgtg ctgaggggaa
2941 gtgccctcgc cagcgtcgcg ccctggagcg gggtctgcag gacgaggatg gctacaggat
3001 gaagtttgcc tgctactacc cccgtgtgga gtacggcttc caggttaagg ttctccgaga
3061 agacagcaga gctgctttcc gcctctttga gaccaagatc acccaagtcc tgcacttcac
3121 caaggatgtc aaggccgctg ctaatcagat gcgcaacttc ctggttcgag cctcctgccg
3181 ccttcgcttg gaacctggga aagaatattt gatcatgggt ctggatgggg ccacctatga
3241 cctcgaggga cacccccagt acctgctgga ctcgaatagc tggatcgagg agatgccctc
3301 tgaacgcctg tgccggagca cccgccagcg ggcagcctgt gcccagctca acgacttcct
3361 ccaggagtat ggcactcagg ggtgccaggt gtgtacagac tacaaagacc atgacggtga
3421 ttataaagat catgacatcg actacaagga tgacgatgac aagtaagaat tctgatgact
3481 cgagataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
3541 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
3601 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg
3661 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
3721 aattccgtgg tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag
3781 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
3841 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga
3901 ggttttttaa agatctggag accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc
3961 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
4021 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
4081 agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
4141 caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg
4201 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt
4261 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
4321 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
4381 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
4441 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact
4501 ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa
4561 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
4621 ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
4681 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
4741 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
4801 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
4861 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
4921 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
4981 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
5041 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
5101 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
5161 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
5221 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
5281 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
5341 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
5401 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
5461 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
5521 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
5581 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
5641 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
5701 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
5761 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
5821 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
5881 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
5941 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
6001 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
6061 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
6121 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
6181 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
6241 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
6301 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
6361 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
6421 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
6481 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
6541 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
6601 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
6661 tggccttttg ctggcctttt gctcacatgt
//
