| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001490 |
| 产品用途: | 5' AAVLINK plasmid for ARHGAP5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ARHGAP5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG 5726 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5726)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5726
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2427
/codon_start=1
/label=ARHGAP5
/translation="VVLIILQSLLTRS*MIRR*RRKPTK*KKIKSRKRKLRTSIHQHVE
IGKVITLGCPSRIWLQLRSPYHYLLRNVWNLLKIQGYVPKDSTVSAGIKLTKTIFKSSL
IKIIISI*CQWK*Q*ML*LEPLKLSLQICQIL*FHILFIQNYWKQQKSRIKQNVFMP*K
KLLRNFIL*TMMYSDT**HI*TGLVSNIKST**QQTTYPSVFGQP**DLILKIESFCLL
LRFINLLLKHSFSSVSFSFTMEKL*KRQTLWLLHHLQTQDSWWNQWCHFSCRHHCNLS*
YNHNYKRILLVL"
CDS 2434..2499
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2515..2946
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2659..2664
/label=SDS
repeat_region 2989..3129
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 2989..3118
/label=AAV2 ITR
rep_origin 3204..3659
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3941..4045
/gene="bla"
/label=AmpR promoter
CDS 4046..4906
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5077..5665
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgg tattgataat
1561 cctgcaatca cttctgacca ggagttagat gataagaaga tgaagaagaa aacccacaaa
1621 gtgaaagaag ataaaaagca gaaaaagaaa actaagaact tcaatccacc aacacgtaga
1681 aattgggaaa gtaattactt tgggatgccc ctccaggatc tggttacagc tgagaagccc
1741 ataccactat ttgttgagaa atgtgtggaa tttattgaag atacagggtt atgtaccgaa
1801 ggactctacc gtgtcagcgg gaataaaact gaccaagaca atattcaaaa gcagtttgat
1861 caagatcata atatcaatct agtgtcaatg gaagtaacag taaatgctgt agctggagcc
1921 cttaaagctt tctttgcaga tctgccagat cctttaattc catattctct tcatccagaa
1981 ctattggaag cagcaaaaat cccggataaa acagaacgtc ttcatgcctt gaaagaaatt
2041 gttaagaaat ttcatcctgt aaactatgat gtattcagat acgtgataac acatctaaac
2101 agggttagtc agcaacataa aatcaaccta atgacagcag acaacttatc catctgtttt
2161 tggccaacct tgatgagacc tgattttgaa aatcgagagt ttctgtctac tactaagatt
2221 catcaatctg ttgttgaaac attcattcag cagtgtcagt ttttctttta caatggagaa
2281 attgtagaaa cgacaaacat tgtggctcct ccaccacctt caaacccagg acagttggtg
2341 gaaccaatgg tgccacttca gttgccgcca ccattgcaac ctcagctgat acaaccacaa
2401 ttacaaacgg atcctcttgg tattatatgt acagactaca aagaccatga cggtgattat
2461 aaagatcatg acatcgacta caaggatgac gatgacaagt aagaattctg atgactcgag
2521 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
2581 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
2641 gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc acggcggaac
2701 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
2761 ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc atctagcttt
2821 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
2881 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
2941 ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag gaacccctag
3001 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
3061 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
3121 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
3181 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
3241 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc
3301 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
3361 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
3421 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
3481 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat
3541 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa
3601 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
3661 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
3721 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
3781 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
3841 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
3901 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
3961 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
4021 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
4081 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
4141 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
4201 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
4261 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
4321 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
4381 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
4441 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
4501 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
4561 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
4621 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
4681 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
4741 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
4801 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
4861 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
4921 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
4981 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
5041 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
5101 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
5161 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
5221 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
5281 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
5341 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
5401 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
5461 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
5521 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
5581 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
5641 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
5701 cttttgctgg ccttttgctc acatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-ARHGAP5-FLAG质粒载体应用举例
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