| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001388 |
| 产品用途: | 3' AAVLINK plasmid for AHDC1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | AHDC1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG 6426 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6426)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6426
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3139
/codon_start=1
/label=AHDC1
/translation="GTTASATSAASAASSSSSSFQPSPENCRQFAGASQWPFRQGYGGL
DWASEAFSQLYNPSFDCHVSEPNVILDISNYTPQKVKQQTAVSETFSESSSDSTQFNQP
VGGGGFRRANSEASSSEGQSSLSSLEKLMMDWNEASSAPGYNWNQSVLFQSSSKPGRGR
RKKVDLFEASHLGFPTSASAAASGYPSKRSTGPRQPRGGRGGGACSAKKERGGAAAKAK
FIPKPQPVNPLFQDSPDLGLDYYSGDSSMSPLPSQSRAFGVGERDPCDFIGPYSMNPST
PSDGTFGQGFHCDSPSLGAPELDGKHFPPLAHPPTVFDAGLQKAYSPTCSPTLGFKEEL
RPPPTKLAACEPLKHGLQGASLGHAAAAQAHLSCRDLPLGQPHYDSPSCKGTAYWYPPG
SAARSPPYEGKVGTGLLADFLGRTEAACLSAPHLASPPATPKADKEPLEMARPPGPPRG
PAAAAAGYGCPLLSDLTLSPVPRDSLLPLQDTAYRYPGFMPQAHPGLGGGPKSGFLGPM
AEPHPEDTFTVTSL"
misc_feature 1584..1589
/label=SDS
misc_feature 1587..1592
/label=SDS
misc_feature 1590..1595
/label=SDS
misc_feature 1593..1598
/label=SDS
misc_feature 1596..1601
/label=SDS
misc_feature 1599..1604
/label=SDS
CDS 3146..3211
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3215..3646
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3359..3364
/label=SDS
repeat_region 3689..3829
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3689..3818
/label=AAV2 ITR
rep_origin 3904..4359
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4641..4745
/gene="bla"
/label=AmpR promoter
CDS 4746..5606
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5777..6365
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggca ccacagcctc
1561 tgccacctct gcagcctctg ccgcctcctc ctcctcctcc tccttccagc cctcgcccga
1621 gaactgtcgg cagtttgcgg gggcttctca gtggcctttc cggcagggct atggaggcct
1681 ggactgggcc tcagaggcct ttagtcagct ctacaatccc agttttgact gccacgtcag
1741 cgagcccaac gtgatcctgg acatctccaa ctacacaccg cagaaggtga agcagcagac
1801 ggctgtgtcg gagaccttct ctgagtcatc ctccgacagc acccagttca atcagccggt
1861 tggtggcggg gggtttcggc gtgccaacag cgaggcctca agtagtgagg gccagtcgag
1921 cctgtccagc ctggagaaac tgatgatgga ctggaacgag gcatcatctg cccccggcta
1981 caactggaac cagagtgtcc tctttcagag tagctccaag ccgggccgtg gacggcggaa
2041 gaaggtggac ctgttcgagg cctcacatct gggcttcccg acatccgcct ctgccgctgc
2101 ctcaggctac ccatccaaac ggagcactgg gccccggcag ccgcgaggtg gacggggcgg
2161 tggggcctgc tcagccaaga aggagcgggg tggcgcagcg gccaaagcca agttcatccc
2221 caagccacag ccagtcaacc cactgttcca ggacagtcct gacctcggcc tggactacta
2281 tagcggggac agcagcatgt caccactgcc ctcacagtcg agggccttcg gcgtgggaga
2341 gcgagacccc tgtgacttca taggacccta ctccatgaac ccgtccacgc cttccgatgg
2401 cacctttggc caaggcttcc actgcgactc gcccagcctg ggtgctcccg agcttgatgg
2461 caagcatttc ccacctctgg cccacccacc cacggtgttt gacgccggcc tgcagaaggc
2521 atactcgccc acctgctcgc ctacactggg cttcaaggaa gagctgcggc caccgcccac
2581 aaagctggct gcctgcgagc ccctcaagca tggactccag ggggccagcc tgggccacgc
2641 agctgcagcc caggcccacc tgagctgccg ggacctgccg ctgggccagc cccactacga
2701 ttcccccagc tgcaagggca cagcctattg gtaccctcca ggctcagctg cccgcagccc
2761 gccctatgaa ggcaaggtgg gtacagggct gctggctgac ttcctgggca ggacggaggc
2821 cgcgtgcctc agtgcccctc acctggctag cccaccagcc acgcccaagg ccgacaagga
2881 gccactggaa atggcccggc cccctggccc accccgtggc cctgctgcag ccgctgctgg
2941 ctatggctgc ccactcctta gtgacttgac cctgtccccc gtgccgaggg actcgctgct
3001 gcccctgcag gacaccgcct acaggtaccc aggctttatg ccccaggcgc atcctggcct
3061 gggtgggggc cccaagagcg gcttcctggg gcccatggcg gaacctcacc ccgaggacac
3121 attcaccgtc acatccctgt gtacagacta caaagaccat gacggtgatt ataaagatca
3181 tgacatcgac tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac
3241 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
3301 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
3361 tccttgtata aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc
3421 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa
3481 atttgtgatg ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc
3541 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
3601 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg
3661 gtgtggcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
3721 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
3781 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
3841 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
3901 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
3961 ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg
4021 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
4081 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
4141 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
4201 gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt
4261 tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat
4321 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
4381 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
4441 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
4501 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
4561 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
4621 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
4681 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
4741 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
4801 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
4861 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
4921 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
4981 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
5041 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
5101 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
5161 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
5221 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
5281 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
5341 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
5401 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
5461 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
5521 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
5581 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
5641 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
5701 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
5761 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
5821 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
5881 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
5941 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
6001 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
6061 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
6121 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
6181 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
6241 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
6301 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
6361 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
6421 acatgt
//
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG载体图谱质粒图谱pdf版和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-AHDC1-FLAG质粒载体应用举例
- 上一篇：pAAVLINKv1-pCALM1-Cre-3'-AHDC1-FLAG
- 下一篇：没有了
