| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001453 |
| 产品用途: | 3' AAVLINK plasmid for ZMYM2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ZMYM2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG 6611 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6611)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6611
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3312
/codon_start=1
/label=ZMYM2
/translation="V*LL*ISRNS*RASSVAWGNETFL*STLLTAFLLSTK*AQHDNSE
RT*KLTL*SGLSDISNQNDRFSTTPFSNT*QRDEEQSSSLQTFNNDKSYLL*TSHADQI
LSDR*YLEDRICSSAYPCACVYPSSYAHVQSEYSCSYYSSCSCASSCFSACSIGQQ*ED
SCSN*GAKKQGFFRCS*YRVAYNDGYDE*RRGENRDNQHQQCNY*NRYNWFRPFEEL*P
RDTVQHA*CTI*TRFGYRNRFSQSC*GA*YGK*IFITTCFWRRI*GTAQTSI*KKGSQE
KGCIRIPVS***F*QFRMQLSFQIYVWRKCMETLGQN*AT**RSSGIR*VKIF*ISKVK
RGSTLSHHS*A*LWVSSFCQ*DPTAKWRELCT*QHLLPLPWNTGVFVWK*SKRQHIY*S
WIPNI*ARIE*NTAKLATKHTSRWVNILSS*RRLSLEDKTTRITLSSSSSEYTVLL*H*
VFWPENSGTTLKTFLWHCV*ALEKKSFNDGKQSVSSIPSVFLVWNR**R*NYYWKKKT*
R**ASI*TN*KHSQSFQMSCENV*MLLV*KSTES*SEDGCFLFATRML*FYR*PCLVYV
YFTGPKHLGKYACTGSSSKRYL**RQL*TG*RHR"
CDS 3319..3384
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3400..3831
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3544..3549
/label=SDS
repeat_region 3874..4014
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3874..4003
/label=AAV2 ITR
rep_origin 4089..4544
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4826..4930
/gene="bla"
/label=AmpR promoter
CDS 4931..5791
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5962..6550
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtgactg ttgtaaatct
1501 caaggaactc ttaaagagcg agttcagtgg cgtggggaaa tgaaacattt ctgtgatcaa
1561 cattgcttac tgcgtttcta ctgtcaacaa aatgagccca acatgacaac tcagaaagga
1621 cctgaaaact tacattatga tcagggttgt cagacatctc gaaccaaaat gacaggttca
1681 gcaccacccc cttctccaac acctaacaaa gagatgaaga acaaagcagt tctttgcaaa
1741 cctttaacaa tgacaaaagc tacttactgt aaacctcaca tgcagaccaa atcttgtcag
1801 acagatgata cttggaggac agaatatgtt ccagtgccta tccctgtgcc tgtgtatatc
1861 ccagttccta tgcacatgta cagtcagaat attcctgttc ctactacagt tcctgttcct
1921 gtgccagttc ctgtttttct gcctgctcca ttggacagca gtgagaagat tcatgcagca
1981 attgaggagc taaaaagcaa ggtttcttca gatgctcttg atacagagtt gcttacaatg
2041 acggatatga tgagtgaaga cgaggggaaa acagagacaa ccaacatcaa cagtgtaatt
2101 attgaaacag atataattgg ttcagacctt ttgaagaact ctgacccaga gacacagtcc
2161 agcatgcctg atgtaccata tgaaccagat ttggatatcg aaatagattt tcccagagct
2221 gctgaggagc ttgatatgga aaatgaattt ttattaccac ctgtttttgg cgaagaatat
2281 gaggaacagc ccagacctcg atctaaaaaa aagggagcca agagaaaggc tgtatcagga
2341 taccagtctc atgatgatag ttctgacaat tcagaatgca gctttccttt caaatatacg
2401 tatggcgtaa atgcatggaa acactgggtc aaaactaggc aacttgatga agatcttctg
2461 gtattagatg agttaaaatc ttctaaatca gtaaagttaa aagaggatct actctctcac
2521 accacagctg agcttaacta tgggttagct cattttgtca atgagatccg acggccaaat
2581 ggagagaatt atgcacctga cagcatctat tacctttgcc ttggaataca ggagtatttg
2641 tgtggaagta atcgaaaaga caacatattt attgatcctg gataccaaac atttgagcaa
2701 gaattgaata aaatactgcg aagctggcaa ccaagcatac ttccagatgg gtcaatattc
2761 tctcgagttg aagaagacta tctctggagg ataaaacaac taggatcaca ctctccagta
2821 gctcttctga atacactgtt ctactttaac actaagtatt ttggcctgaa aacagtggaa
2881 caacacttaa gactttcctt tggcactgtg tttaggcatt ggaaaaaaaa tcctttaacg
2941 atggaaaaca aagcgtgtct tcgataccaa gtgtcttcct tgtgtggaac agataatgaa
3001 gataaaatta ctactggaaa aagaaaacat gaagatgatg agccagtatt tgaacaaatt
3061 gaaaacacag ccaatccttc cagatgtcct gtgaaaatgt ttgaatgcta cttgtctaaa
3121 agtccacaga atcttaatca gaggatggat gttttttatt tgcaaccaga atgctctagt
3181 tctacagata gccctgtctg gtatacgtct acttcactgg accgaaacac cttggaaaat
3241 atgcttgtac gggttcttct agtaaaagat atttatgata aagacaatta tgaactggat
3301 gaagacacag actgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
3361 gactacaagg atgacgatga caagtaagaa ttctgatgac tcgagataat caacctctgg
3421 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
3481 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
3541 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
3601 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
3661 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
3721 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
3781 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
3841 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
3901 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
3961 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
4021 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
4081 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
4141 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
4201 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
4261 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
4321 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
4381 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
4441 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
4501 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
4561 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
4621 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
4681 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
4741 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
4801 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
4861 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
4921 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
4981 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
5041 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
5101 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
5161 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
5221 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
5281 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
5341 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
5401 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
5461 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
5521 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
5581 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
5641 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
5701 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
5761 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
5821 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
5881 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
5941 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
6001 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
6061 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
6121 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
6181 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
6241 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
6301 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
6361 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
6421 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
6481 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
6541 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
6601 tgctcacatg t
//
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG载体图谱质粒图谱pdf版和pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG载体序列质粒序列等相关资料下载
pAAVLINKv1-pCALM1-Cre-3'-ZMYM2-FLAG质粒载体应用举例
- 上一篇：pAAVLINK-EF1a-HA-5'-ZMYM2
- 下一篇：没有了
