| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001537 |
| 产品用途: | 3' AAVLINK plasmid for UNC13A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | UNC13A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-UNC13A-FLAG 6895 bp DNA circular SYN 17-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6895)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6895
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3608
/codon_start=1
/label=UNC13A
/translation="VVKDCVKACLNSTYEYIFNNCHELYSREYQTDPAKKGEVLPEEQG
PSIKNLDFWSKLITLIVSIIEEDKNSYTPCLNQFPQELNVGKISAEVMWNLFAQDMKYA
MEEHDKHRLCKSADYMNLHFKVKWLYNEYVTELPAFKDRVPEYPAWFEPFVIQWLDENE
EVSRDFLHGALERDKKDGFQQTSEHALFSCSVVDVFSQLNQSFEIIKKLECPDPQIVGH
YMRRFAKTISNVLLQYADIISKDFASYCSKEKEKVPCILMNNTQQLRVQLEKMFEAMGG
KELDAEASDILKELQVKLNNVLDELSRVFATSFQPHIEECVKQMGDILSQVKGTGNVPA
SACSSVAQDADNVLQPIMDLLDSNLTLFAKICEKTVLKRVLKELWKLVMNTMEKTIVLP
PLTDQTMIGNLLRKHGKGLEKGRVKLPSHSDGTQMIFNAAKELGQLSKLKDHMVREEAK
SLTPKQCAVVELALDTIKQYFHAGGVGLKKTFLEKSPDLQSLRYALSLYTQATDLLIKT
FVQTQSAQGLGVEDPVGEVSVHVELFTHPGTGEHKVTVKVVAANDLKWQTSGIFRPFIE
VNIIGPQLSDKKRKFATKSKNNSWAPKYNESFQFTLSADAGPECYELQVCVKDYCFARE
DRTVGLAVLQLRELAQRGSAACWLPLGRRIHMDDTGLTVLRILSQRSNDEVAKEFVKLK
SDTRSAEEGGAAPAP"
misc_feature 1969..1974
/label=SDS
misc_feature 2221..2226
/label=SDS
misc_feature 3583..3588
/label=SDS
CDS 3615..3680
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3684..4115
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3828..3833
/label=SDS
repeat_region 4158..4298
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4158..4287
/label=AAV2 ITR
rep_origin 4373..4828
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5110..5214
/gene="bla"
/label=AmpR promoter
CDS 5215..6075
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6246..6834
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggtaaag gactgtgtga
1501 aagcctgcct taattctacc tacgagtaca tcttcaataa ctgccatgaa ctgtacagcc
1561 gggagtacca gacagacccg gccaagaagg gggaagttct cccagaggaa caggggccca
1621 gcatcaagaa cctcgacttc tggtccaagc tgattaccct catagtgtcc atcattgagg
1681 aagacaagaa ttcctacact ccctgcctca accagtttcc ccaggagctg aatgtgggta
1741 aaatcagcgc tgaagtgatg tggaatctgt ttgcccaaga catgaagtac gccatggagg
1801 agcacgacaa gcatcgtcta tgcaagagtg ccgactacat gaacctccac ttcaaggtga
1861 aatggctcta caatgagtat gtgacggaac ttcccgcctt caaggaccgc gtgcctgagt
1921 accctgcatg gtttgaaccc ttcgtcatcc agtggctgga tgagaatgag gaggtgtccc
1981 gggatttcct gcacggtgcc ctggagcgag acaagaagga tgggttccag cagacctcag
2041 agcatgccct attctcctgc tccgtggtgg atgtcttctc ccaactcaac cagagctttg
2101 aaatcatcaa gaaactcgag tgtcccgacc ctcagatcgt ggggcactac atgaggcgct
2161 ttgccaagac catcagtaat gtgctcctcc agtatgcaga catcatctcc aaggactttg
2221 cctcctactg ctccaaggag aaggagaaag tgccctgcat tctcatgaat aacactcaac
2281 agctacgagt tcagctggag aagatgttcg aagccatggg aggaaaggag ctggatgctg
2341 aagccagtga catcctgaag gagcttcagg tgaaactcaa taacgtcttg gatgagctca
2401 gccgggtgtt tgctaccagc ttccagccgc acattgaaga gtgtgtcaaa cagatgggtg
2461 acatccttag ccaggttaag ggcacaggca atgtgccagc cagtgcctgc agcagcgtgg
2521 cccaggacgc ggacaatgtg ttgcagccca tcatggacct gctggacagc aacctgaccc
2581 tctttgccaa aatctgtgag aagactgtgc tgaagcgagt gctgaaggag ctgtggaagc
2641 tggttatgaa caccatggag aaaaccatcg tcctgccgcc cctcactgac cagacgatga
2701 tcgggaacct cttgagaaaa catggcaagg gattagaaaa gggcagggtg aaattgccaa
2761 gccactcaga cggaacccag atgatcttca atgcagccaa ggagctgggt cagctgtcca
2821 aactcaagga tcacatggta cgagaagaag ccaagagctt gaccccaaag cagtgcgcgg
2881 ttgttgagtt ggccctggac accatcaagc aatatttcca cgcgggtggc gtgggcctca
2941 agaagacctt cctggagaag agcccggacc tgcaatcctt gcgctatgcc ctgtcgctct
3001 acacgcaggc caccgacctg ctaatcaaga cctttgtaca gacgcaatcg gcccagggct
3061 tgggtgtaga agaccctgtg ggtgaagtct ctgtccatgt tgagctgttc actcatccag
3121 gaactgggga acacaaggtc acagtgaaag tggtggctgc caatgacctc aagtggcaga
3181 cttctggcat cttccggccg ttcatcgagg tcaacatcat tgggccccag ctcagcgaca
3241 agaaacgcaa gtttgcgacc aaatccaaga acaatagctg ggctcccaag tacaatgaga
3301 gcttccagtt cacgctgagc gccgacgcgg gtcccgagtg ctatgagctg caggtgtgcg
3361 tcaaggacta ctgcttcgcg cgcgaggacc gcacggtggg gctggccgtg ctgcagctgc
3421 gtgagctggc ccagcgcggg agcgccgcct gctggctgcc gctcggccgc cgcatccaca
3481 tggacgacac gggcctcacg gtgctgcgaa tcctctcgca gcgcagcaac gacgaggtgg
3541 ccaaggagtt cgtgaagctc aagtcggaca cgcgctccgc cgaggagggc ggtgccgcgc
3601 ctgcgccttg tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact
3661 acaaggatga cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga
3721 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
3781 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
3841 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
3901 aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc
3961 tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat
4021 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
4081 ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt
4141 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
4201 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
4261 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4321 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4381 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4441 gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4501 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4561 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4621 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4681 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
4741 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
4801 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
4861 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
4921 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
4981 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5041 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5101 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5161 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5221 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5281 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5341 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5401 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5461 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5521 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5581 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5641 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
5701 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
5761 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
5821 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
5881 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
5941 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6001 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6061 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6121 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6181 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6241 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6301 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6361 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
6421 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6481 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6541 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6601 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6661 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
6721 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
6781 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
6841 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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