| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001381 |
| 产品用途: | 3' AAVLINK plasmid for SYNGAP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SYNGAP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SYNGAP1-FLAG 6985 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6985)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6985
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3698
/codon_start=1
/label=SYNGAP1
/translation="VVNSHCVFPRELKEVFASWRLRCAERGREDIADRLISASLFLRFL
CPAIMSPSLFGLMQEYPDEQTSRTLTLIAKVIQNLANFSKFTSKEDFLGFMNEFLELEW
GSMQQFLYEISNLDTLTNSSSFEGYIDLGRELSTLHALLWEVLPQLSKEALLKLGPLPR
LLNDISTALRNPNIQRQPSRQSERPRPQPVVLRGPSAEMQGYMMRDLNSSMDMARLPSP
TKEKPPPPPPGGGKDLFYVSRPPLARSSPAYCTSSSDITEPEQKMLSVNKSVSMLDLQG
DGPGGRLNSSSVSNLAAVGDLLHSSQASLTAALGLRPAPAGRLSQGSGSSITAAGMRLS
QMGVTTDGVPAQQLRIPLSFQNPLFHMAADGPGPPGGHGGGGGHGPPSSHHHHHHHHHH
RGGEPPGDTFAPFHGYSKSEDLSSGVPKPPAASILHSHSYSDEFGPSGTDFTRRQLSLQ
DNLQHMLSPPQITIGPQRPAPSGPGGGSGGGSGGGGGGQPPPLQRGKSQQLTVSAAQKP
RPSSGNLLQSPEPSYGPARPRQQSLSKEGSIGGSGGSGGGGGGGLKPSITKQHSQTPST
LNPTMPASERTVAWVSNMPHLSADIESAHIEREEYKLKEYSKSMDESRLDREYEEEIHS
LKERLHMSNRKLEEYERRLLSQEEQTSKILMQYQARLEQSEKRLRQQQAEKDSQIKSII
GRLMLVEEELRRDHPAMAEPLPEPKKRLLDAQRGSFPPWVQQTRV"
misc_feature 1519..1524
/label=SDS
misc_feature 1747..1752
/label=SDS
misc_feature 1943..1948
/label=SDS
CDS 2649..2678
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
misc_feature 3112..3117
/label=SDS
misc_feature 3370..3375
/label=SDS
misc_feature 3583..3588
/label=SDS
CDS 3705..3770
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3774..4205
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3918..3923
/label=SDS
repeat_region 4248..4388
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4248..4377
/label=AAV2 ITR
rep_origin 4463..4918
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5200..5304
/gene="bla"
/label=AmpR promoter
CDS 5305..6165
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6336..6924
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtggtcaac tcccactgcg
1501 tgttcccgag ggagctgaag gaggtgtttg cttcatggcg gctgcgctgc gcagagcgag
1561 gccgggagga catcgcagac aggcttatca gcgcctcact cttcctgcgc ttcctctgcc
1621 cagcgattat gtcgcccagt ctctttgggc ttatgcagga gtacccagat gagcagacct
1681 cacgaaccct caccctcatt gccaaggtca tccagaacct ggccaacttt tccaagttta
1741 cctcaaagga ggactttctg ggcttcatga atgagtttct ggagctggaa tggggttcca
1801 tgcagcagtt tttgtatgag atctccaatc tggacacgct aaccaacagc agtagctttg
1861 agggttacat cgacttgggc cgagagctct ccacactgca tgccctactc tgggaggtgc
1921 tgccccagct cagcaaggaa gccctcctga agctgggtcc actgccccgg ctcctcaacg
1981 acatcagcac agctctgagg aaccccaaca tccaaaggca gccaagccgc cagagtgagc
2041 ggccccggcc tcagcctgtg gtactgcggg ggccatcggc tgagatgcag ggctacatga
2101 tgcgggacct caacagctct atggacatgg ctcgcctccc ctccccaacc aaggaaaagc
2161 cacccccacc accgcctggt ggtggtaaag acctgttcta tgtaagccgt ccacccctgg
2221 cccgttcctc accagcatac tgcacgagca gctcggacat cacagagcca gagcagaaga
2281 tgctgagtgt caacaagagt gtgtccatgc tggacttaca gggtgatggg cctggtggcc
2341 gcctcaacag cagcagtgtt tcgaacctgg cggccgtagg ggacctgctg cactcaagcc
2401 aggcctcgct gacagcagcc ttggggctac ggcctgcgcc tgccggacgc ctctcccagg
2461 ggagtggctc atccatcacg gcggctggca tgcgcctcag ccagatgggt gtcaccacag
2521 acggtgtccc tgcccagcaa ctgcgaatcc ccctctcctt ccagaaccct ctcttccaca
2581 tggctgctga tgggccaggt cccccaggcg gccatggagg gggcggtggc catggcccac
2641 cttcctccca tcaccaccac caccaccatc accaccaccg aggtggagag ccccctgggg
2701 acacctttgc cccattccat ggctatagca agagtgagga cctctcttcc ggggtcccca
2761 agccccctgc tgcctccatc cttcatagcc acagctacag tgatgagttt ggaccctctg
2821 gcactgactt cacccgtcgg cagctttcac tccaggacaa cctgcagcac atgctgtccc
2881 ctccccagat caccattggt ccccagaggc cagccccctc agggcctgga ggtgggagcg
2941 gtgggggcag cggtgggggt ggcgggggcc agccgcctcc attgcagagg ggcaagtctc
3001 agcagttgac agtcagcgca gcccagaaac cccggccatc cagcgggaat ctattgcagt
3061 ccccagagcc aagttatggc cccgcccgtc cacggcaaca gagcctcagc aaggagggca
3121 gcattggggg cagcgggggc agcggtggcg gagggggtgg ggggctgaag ccctccatca
3181 ccaagcagca ttctcagaca ccatccacat tgaaccccac aatgccagcc tctgagcgga
3241 cagtggcctg ggtctccaac atgcctcacc tgtcggctga catcgagagt gcccacatcg
3301 agcgggaaga gtacaagctc aaggagtact caaaatcgat ggatgagagc cggctggata
3361 gggagtacga ggaggagatt cactcactga aagagcggct gcacatgtcc aaccggaagc
3421 tggaagagta tgagcggagg ctgctgtccc aggaagaaca aaccagcaaa atcctgatgc
3481 agtatcaggc ccgactggag cagagtgaga agaggctaag gcagcagcag gcagagaagg
3541 attcccagat caagagcatc attggcaggc tgatgctggt ggaggaggag ctgcgccggg
3601 accaccccgc catggctgag ccgctgccag aacccaagaa gaggctgctc gacgctcaga
3661 gaggcagctt ccccccttgg gtccaacaaa cccgcgtgtg tacagactac aaagaccatg
3721 acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga
3781 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
3841 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
3901 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
3961 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
4021 cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta
4081 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
4141 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt
4201 tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt
4261 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
4321 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg
4381 cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
4441 catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4501 gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct
4561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg
4681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
4741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc
4801 tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat
4861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta
4921 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg
4981 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa
5041 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc
5101 gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
5161 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
5221 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
5281 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
5341 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
5401 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
5461 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
5521 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
5581 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
5641 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
5701 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
5761 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
5821 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
5881 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
5941 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
6001 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
6061 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
6121 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
6181 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
6241 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
6301 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
6361 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
6421 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
6481 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
6541 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
6601 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
6661 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
6721 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6781 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6841 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6901 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6961 ttttgctggc cttttgctca catgt
//
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