| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001393 |
| 产品用途: | 3' AAVLINK plasmid for SMARCA4 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SMARCA4 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SMARCA4-FLAG 6763 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6763)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6763
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3476
/codon_start=1
/label=SMARCA4
/translation="GVLLTDGSEKDKKGKGGTKTLMNTIMQLRKICNHPYMFQHIEESF
SEHLGFTGGIVQGLDLYRASGKFELLDRILPKLRATNHKVLLFCQMTSLMTIMEDYFAY
RGFKYLRLDGTTKAEDRGMLLKTFNEPGSEYFIFLLSTRAGGLGLNLQSADTVIIFDSD
WNPHQDLQAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQ
KSSSHERRAFLQAILEHEEQDESRHCSTGSGSASFAHTAPPPAGVNPDLEEPPLKEEDE
VPDDETVNQMIARHEEEFDLFMRMDLDRRREEARNPKRKPRLMEEDELPSWIIKDDAEV
ERLTCEEEEEKMFGRGSRHRKEVDYSDSLTEKQWLKKITGKDIHDTASSVARGLQFQRG
LQFCTRASKAIEEGTLEEIEEEVRQKKSSRKRKRDSDAGSSTPTTSTRSRDKDDESKKQ
KKRGRPPAEKLSPNPPNLTKKMKKIVDAVIKYKDSSSGRQLSEVFIQLPSRKELPEYYE
LIRKPVDFKKIKERIRNHKYRSLNDLEKDVMLLCQNAQTFNLEGSLIYEDSIVLQSVFT
SVRQKIEKEDDSEGEESEEEEEGEEEGSESESRSVKVKIKLGRKEKAQDRLKGGRRRPS
RGSRAKPVVSDDDSEEEQEEDRSGSGSEED"
misc_feature 2311..2316
/label=SDS
misc_feature 2368..2373
/label=SDS
misc_feature 2416..2421
/label=SDS
misc_feature 2455..2460
/label=SDS
misc_feature 2518..2523
/label=SDS
misc_feature 2521..2526
/label=SDS
misc_feature 2524..2529
/label=SDS
misc_feature 2563..2568
/label=SDS
misc_feature 2713..2718
/label=SDS
misc_feature 2740..2745
/label=SDS
misc_feature 3232..3237
/label=SDS
misc_feature 3262..3267
/label=SDS
misc_feature 3265..3270
/label=SDS
misc_feature 3280..3285
/label=SDS
misc_feature 3430..3435
/label=SDS
misc_feature 3442..3447
/label=SDS
CDS 3483..3548
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3552..3983
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3696..3701
/label=SDS
repeat_region 4026..4166
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4026..4155
/label=AAV2 ITR
rep_origin 4241..4696
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4978..5082
/gene="bla"
/label=AmpR promoter
CDS 5083..5943
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6114..6702
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcgtgctg ctgactgatg
1501 gctccgagaa ggacaagaag ggcaaaggcg gcaccaagac cctgatgaac accatcatgc
1561 agctgcggaa gatctgcaac cacccctaca tgttccagca catcgaggag tccttttccg
1621 agcacttggg gttcactggc ggcattgtcc aagggctgga cctgtaccga gcctcgggta
1681 aatttgagct tcttgataga attcttccca aactccgagc aaccaaccac aaagtgctgc
1741 tgttctgcca aatgacctcc ctcatgacca tcatggaaga ttactttgcg tatcgcggct
1801 ttaaatacct caggcttgat ggaaccacga aggcggagga ccggggcatg ctgctgaaaa
1861 ccttcaacga gcccggctct gagtacttca tcttcctgct cagcacccgg gctggggggc
1921 tcggcctgaa cctccagtcg gcagacactg tgatcatttt tgacagcgac tggaatcctc
1981 accaggacct gcaagcgcag gaccgagccc accgcatcgg gcagcagaac gaggtgcgtg
2041 tgctccgcct ctgcaccgtc aacagcgtgg aggagaagat cctagctgca gccaagtaca
2101 agctcaacgt ggaccagaag gtgatccagg ccggcatgtt cgaccagaag tcctccagcc
2161 atgagcggcg cgccttcctg caggccatcc tggagcacga ggagcaggat gagagcagac
2221 actgcagcac gggcagcggc agtgccagct tcgcccacac tgcccctccg ccagcgggcg
2281 tcaaccccga cttggaggag ccacctctaa aggaggaaga cgaggtgccc gacgacgaga
2341 ccgtcaacca gatgatcgcc cggcacgagg aggagtttga tctgttcatg cgcatggacc
2401 tggaccgcag gcgcgaggag gcccgcaacc ccaagcggaa gccgcgcctc atggaggagg
2461 acgagctccc ctcgtggatc atcaaggacg acgcggaggt ggagcggctg acctgtgagg
2521 aggaggagga gaagatgttc ggccgtggct cccgccaccg caaggaggtg gactacagcg
2581 actcactgac ggagaagcag tggctcaaga aaattacagg aaaagatatc catgacacag
2641 ccagcagtgt ggcacgtggg ctacaattcc agcgtggcct tcagttctgc acacgtgcgt
2701 caaaggccat cgaggagggc acgctggagg agatcgaaga ggaggtccgg cagaagaaat
2761 catcacggaa gcgcaagcga gacagcgacg ccggctcctc caccccgacc accagcaccc
2821 gcagccgcga caaggacgac gagagcaaga agcagaagaa gcgcgggcgg ccgcctgccg
2881 agaaactctc ccctaaccca cccaacctca ccaagaagat gaagaagatt gtggatgccg
2941 tgatcaagta caaggacagc agcagtggac gtcagctcag cgaggtcttc atccagctgc
3001 cctcgcgaaa ggagctgccc gagtactacg agctcatccg caagcccgtg gacttcaaga
3061 agataaagga gcgcattcgc aaccacaagt accgcagcct caacgaccta gagaaggacg
3121 tcatgctcct gtgccagaac gcacagacct tcaacctgga gggctccctg atctatgaag
3181 actccatcgt cttgcagtcg gtcttcacca gcgtgcggca gaaaatcgag aaggaggatg
3241 acagtgaagg cgaggagagt gaggaggagg aagagggcga ggaggaaggc tccgaatccg
3301 aatctcggtc cgtcaaagtg aagatcaagc ttggccggaa ggagaaggca caggaccggc
3361 tgaagggcgg ccggcggcgg ccgagccgag ggtcccgagc caagccggtc gtgagtgacg
3421 atgacagtga ggaggaacaa gaggaggacc gctcaggaag tggcagcgaa gaagactgta
3481 cagactacaa agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg
3541 atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg
3601 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
3661 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
3721 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
3781 gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt
3841 tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
3901 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
3961 gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg
4021 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
4081 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
4141 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
4201 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
4261 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
4321 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4381 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4441 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4501 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4561 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4621 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4681 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4741 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4801 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4861 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4921 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4981 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
5041 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
5101 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
5161 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
5221 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
5281 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
5341 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5401 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5461 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5521 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5581 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5641 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5701 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5761 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5821 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5881 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5941 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
6001 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
6061 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
6121 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
6181 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
6241 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
6301 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6361 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6421 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6481 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6541 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6601 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6661 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6721 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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