| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001396 |
| 产品用途: | 3' AAVLINK plasmid for SETD1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: |
pCALM1
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETD1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SETD1A-FLAG 6593 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6593)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6593
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3306
/codon_start=1
/label=SETD1A
/translation="GCRLPSHTPARTGGVSSKACRPHGGVTPQCASASPRTTCWAPGPC
PTPR*ASLFSHPPPAPTQETPENCLLLCHRGGASPGAPSSHTAAGQVSRPSLPQGSPGR
GADHPQPAPGPRISGQELARGGVPRRPEPGWRPRPPHRGRGG*ARDRGGPGGPGRPGPD
PCPARAACPACC*RLRGHRDIGRGRAP*APAQPHPPGAQLCPGRQAHAPCASPAAPGAS
ARTRRPLQFPS**GPGGPRGGGG*GGGAQAAATAAAAGGGRRGGGGRGGGRGGGVL*QQ
QQQRWGGRPPEAQPPLPRPAPPPSAPTPAATAPRLRATQ*V*TDDHPV*HLELGPGLRG
HELPAAYVRAAAAADKRG*LAQRHSLGPSHNHQPDHPKTQAAAPGWAPGAPDRLSPQRR
LLPHQQEGEGQVPGRVPSLGPAAGGRGHSGDEPRAVRAPVRAAAAAERHRYLRHHGQ*P
AETQPAQVPEEEAPIWPEPDPRVGSVCHGTHCC*RDGHRIRGSEHPSDGGRHAGEALRA
GGHWQQLPVPGGPRHHHRCHQVWQPGQIHQPLLHA*LLRQGHHHRVPEEDRDLLQAAHW
RGRGDHLRLQVPTGRQQDPVSVWHRELPGLPK"
misc_feature 1517..1522
/label=SDS
misc_feature 1653..1658
/label=SDS
misc_feature 1853..1858
/label=SDS
misc_feature 1866..1871
/label=SDS
misc_feature 1913..1918
/label=SDS
misc_feature 2073..2078
/label=SDS
misc_feature 2261..2266
/label=SDS
misc_feature 2273..2278
/label=SDS
misc_feature 2300..2305
/label=SDS
misc_feature 2303..2308
/label=SDS
misc_feature 3032..3037
/label=SDS
CDS 3313..3378
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3382..3813
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3526..3531
/label=SDS
repeat_region 3856..3996
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3856..3985
/label=AAV2 ITR
rep_origin 4071..4526
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4808..4912
/gene="bla"
/label=AmpR promoter
CDS 4913..5773
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5944..6532
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggttgcagg ctccccagtc
1501 acacccctgc ccgaacagga ggcgtctcca gcaaggcctg caggccccac ggaggagtca
1561 ccccccagtg cgcctctgcg tcccccagaa ccacctgctg ggcccccggc ccctgcccca
1621 cgccccgatg agcgtccctc ttctcccatc cccctcctgc ccccacccaa gaaacgccgg
1681 aaaactgtct ccttctctgc catcgaggtg gtgccagccc cggagccccc tccagccaca
1741 ccgccgcagg ccaagtttcc cggcccagcc tcccgcaagg ctccccgggg cgtggagcgg
1801 accatccgca acctgcccct ggaccacgca tctctggtca agagttggcc cgaggaggtg
1861 tcccgaggag gccggagccg ggctggaggc cgaggccgcc tcaccgagga agaggaggct
1921 gagccaggga cagaggtgga cctggcggtc ctggccgacc tggccctgac ccctgcccgg
1981 cgcgggctgc ctgccctgcc tgctgttgaa gactcagagg ccacagagac atcggacgag
2041 gccgagcgcc ctaggcccct gctcagccac atcctcctgg agcacaacta tgccctggcc
2101 gtcaagccca cgccccctgc gccagccctg cggcccccgg agccagtgcc cgcacccgcc
2161 gccctcttca gttccccagc tgatgaggtc ctggaggccc ccgaggtggt ggtggctgag
2221 gcggaggagc ccaagccgca gcaactgcag cagcagcggg aggagggcga agaggagggg
2281 gaggaagagg gggaggaaga ggaggaggag tcctctgaca gcagcagcag cagcgatggg
2341 gagggcgccc tccggaggcg cagcctccgc tcccacgccc ggcgccgccg ccctccgccc
2401 ccacccccgc cgccaccgcc ccgcgcctac gagccacgca gtgagtttga acagatgacc
2461 atcctgtatg acatttggaa ctcgggcctg gactcagagg acatgagtta cctgcggctt
2521 acgtacgagc ggctgctgca gcagacaagc ggggctgact ggctcaacga cactcactgg
2581 gtccatcaca caatcaccaa cctgaccacc ccaaaacgca agcggcggcc ccaggatggg
2641 ccccgggagc accagacagg ctcagcccgc agcgaaggct actaccccat cagcaagaag
2701 gagaaggaca agtacctgga cgtgtgccca gtctcggccc ggcagctgga gggcgtggac
2761 actcagggga cgaaccgcgt gctgtccgag cgccggtccg agcagcggcg gctgctgagc
2821 gccatcggta cctccgccat catggacagt gacctgctga aactcaacca gctcaagttc
2881 cggaagaaga agctccgatt tggccggagc cggatccacg agtggggtct gtttgccatg
2941 gaacccattg ctgctgacga gatggtcatc gaatacgtgg gtcagaacat ccgtcagatg
3001 gtggccgaca tgcgggagaa gcgctacgtg caggagggca ttggcagcag ctacctgttc
3061 cgggtggacc acgacaccat catcgatgcc accaagtgtg gcaacctggc cagattcatc
3121 aaccactgct gcacgcctaa ctgctacgcc aaggtcatca ccatcgagtc ccagaagaag
3181 atcgtgatct actccaagca gcccattggc gtggacgagg agatcaccta cgactacaag
3241 ttcccactgg aagacaacaa gatcccgtgt ctgtgtggca cagagagctg ccggggctcc
3301 ctaaactgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
3361 aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa
3421 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
3481 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
3541 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
3601 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
3661 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
3721 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
3781 caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc
3841 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
3901 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
3961 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4021 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4081 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4141 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4201 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4261 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4321 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4381 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4441 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4501 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4561 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
4621 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
4681 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
4741 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
4801 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
4861 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
4921 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
4981 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5041 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5101 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5161 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5221 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5281 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5341 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5401 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5461 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5521 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
5581 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
5641 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
5701 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
5761 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
5821 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
5881 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
5941 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6001 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6061 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6121 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6181 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6241 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6301 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6361 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6421 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6481 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6541 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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