| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001384 |
| 产品用途: | 3' AAVLINK plasmid for SETBP1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SETBP1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-SETBP1-FLAG 6148 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6148)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6148
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2861
/codon_start=1
/label=SETBP1
/translation="VGSASLSSGRLHKRKHKHKHKHKEDRILGTHDNLSGLFAGKATGF
SSHILSERLSSADKELPLVSEKNKHKEKQKHQHSEAGHKASKNNFEVDTLSTLSLSDAQ
HWTQAKEKGDLSSEPVDSCTKRYSGSGGDGGSTRSENLDVFSEMNPSNDKWDSDVSGSK
RRSYEGFGTYREKDIQAFKMNRKERSSYDSSMSPGMPSPHLKVDQTAVHSKNEGSVPTM
MTRKKPAAVDSVTIPPAPVLSLLAASAATSDAVGSSLKKRFKRREIEAIQCEVRKMCNY
TKILSTKKNLDHVNKILKAKRLQRQSKTGNNFVKKRRGRPRKQPTQFDEDSRDQMPVLE
KCIDLPSKRGQKPSLSPLVLEPAASQDTIMATIEAVIHMAREAPPLPPPPPPPLPPPPP
PPLPPPPPLPKTPRGGKRKHKPQAPAQPPQQSPPQQPLPQEEEVKAKRQRKSRGSESEV
LP"
misc_feature 1971..1976
/label=SDS
misc_feature 2803..2808
/label=SDS
CDS 2868..2933
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2937..3368
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3081..3086
/label=SDS
repeat_region 3411..3551
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3411..3540
/label=AAV2 ITR
rep_origin 3626..4081
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4363..4467
/gene="bla"
/label=AmpR promoter
CDS 4468..5328
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5499..6087
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtaggcagt gccagtctgt
1501 ccagtggtcg gctccataag aggaaacaca aacacaagca taagcacaag gaagaccgga
1561 tcctagggac ccatgacaac ctgagtggtc tttttgcagg caaagccaca ggcttctcca
1621 gccacatcct gagcgagcgg ctgagtagcg cagacaaaga gctcccgctg gtgagtgaga
1681 agaacaagca taaggagaaa cagaagcacc agcacagcga agccggccac aaagcttcta
1741 agaacaactt tgaggtggac accctgtcta cactgtcact ttccgacgcc cagcattgga
1801 cacaggccaa ggaaaaagga gacttgagca gtgagcctgt ggactcatgc acgaaaagat
1861 actctggcag tggcggggat ggtggcagca cgagatcgga gaacctggac gtgttcagtg
1921 aaatgaaccc ttcgaatgac aagtgggaca gtgacgtgag tgggagtaaa aggaggagct
1981 atgaaggctt tggaacgtac agggaaaagg acatccaagc cttcaagatg aaccgcaagg
2041 agagaagttc ttatgactcc tccatgtctc cagggatgcc aagtccccac ttaaaagtgg
2101 accagacagc agtgcatagt aagaacgaag gctcagtgcc caccatgatg accaggaaga
2161 agccagccgc agttgacagt gttacaattc caccagcccc agtgttatct ctccttgctg
2221 catctgcagc aacgtcggat gcagtcggct cctccctgaa gaagaggttc aagcggcggg
2281 agatcgaagc catccagtgc gaagtgcgga agatgtgcaa ctacaccaag atcctgtcca
2341 ccaagaagaa cctggaccac gtgaacaaga tcctgaaggc caagcggctg cagagacaat
2401 caaaaacagg caacaacttc gtgaagaaga ggcgcgggcg tcccaggaag cagcccaccc
2461 agttcgatga ggactccaga gaccaaatgc cggtgctgga aaaatgcatc gacctgccca
2521 gcaaaagagg ccagaagccc agcctgagcc cgctggtgct ggagcccgcc gccagccaag
2581 acaccatcat ggccaccatc gaggcggtca tccacatggc ccgggaggcg ccgcccctgc
2641 ccccgccacc gccgccgccc ctgccgccac cgccgccacc acccctgccc ccgccacccc
2701 ctctacccaa gaccccccga ggcggaaaga ggaaacacaa accgcaggcc cccgctcagc
2761 ccccacagca gtcgcccccg cagcagcccc ttccccagga agaggaggtg aaagccaaaa
2821 ggcagaggaa gtcccgaggg agtgagagcg aggtccttcc ctgtacagac tacaaagacc
2881 atgacggtga ttataaagat catgacatcg actacaagga tgacgatgac aagtaactcg
2941 agataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
3001 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
3061 ccgtatggct ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga
3121 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
3181 ttccgtggtg tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct
3241 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
3301 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
3361 ttttttaaag atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct
3421 agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc
3481 aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag
3541 ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
3601 ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt
3661 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc cgctcctttc
3721 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
3781 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
3841 ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
3901 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaactct
3961 atctcgggct attcttttga tttataaggg attttgccga tttcggtcta ttggttaaaa
4021 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt
4081 ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
4141 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
4201 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
4261 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
4321 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
4381 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
4441 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
4501 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
4561 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
4621 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
4681 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
4741 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
4801 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
4861 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
4921 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
4981 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
5041 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
5101 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
5161 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
5221 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
5281 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
5341 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
5401 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
5461 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
5521 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5581 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5641 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5701 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5761 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
5821 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
5881 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
5941 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
6001 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
6061 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6121 gccttttgct ggccttttgc tcacatgt
//
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