| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001456 |
| 产品用途: | 3' AAVLINK plasmid for ROBO2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ROBO2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-ROBO2-FLAG 6756 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6756)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 8, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6756
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3469
/codon_start=1
/label=ROBO2
/translation="VFNTG*RLQLVPVQGLE*RVSHSQ**SGDAMKLSLLKTITA*LSK
SLMW*SNQPL*LVLVVPAG*F*WVLAYGCIGEERRGRDSVIMLLRFKEEMED**AMEAV
QVFSMLVIPAIHGLLILGQPRACQ*IIATVAQMRLEILAVEMCCHQFQAKGIKQQRCSQ
MEPFIVALTSLPKPVTTVPAK*HRLPHMPRHRSCIPTAYMNWLSICLIHNGKAQFSKKQ
I*WDLVILYLIRTKVTMVGKVEKRRKIKTLLNHRKTMDPLGPMSLYLPPQSSPFLARSW
NTMQWNNKKMAMTVIAGAHHCQYKLTYTKVWKMNWKKMMIGSQHLLFEAWLLLLLSPLD
SSPLQLLLHPHGKRCNPCCRLTWMS*QEPISLI*QNKHGTFKAIINLHSLQFHR*VMCL
EP*FLIWKRMLQMMMPTTKRKL*KSPGP*EHWTRLLDPAWTI*TAL*QEKPLPPLKDLD
LPAHFLLTVTPVQP*VKVRGLGPLKNTREGGWTNNQHCLIEGKE*QMIFHHHQIPRQVR
V*GSK*ARASRLVTWKTQQREKEAL*RDNMHPA*KTQRAHWIVQLEPP*SGSDKPRNG*
APQNDKKIYGKPHTNKVSDQRRPWCPIASPVSHLQVATAHQEQLLLRDPLDLGKPRC*E
QATSAMPATFLT*DIWAPTVKDSLQVN"
misc_feature 2456..2461
/label=SDS
misc_feature 2835..2840
/label=SDS
misc_feature 3270..3275
/label=SDS
CDS 3476..3541
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3545..3976
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3689..3694
/label=SDS
repeat_region 4019..4159
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4019..4148
/label=AAV2 ITR
rep_origin 4234..4689
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4971..5075
/gene="bla"
/label=AmpR promoter
CDS 5076..5936
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6107..6695
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtattcaat accgggtaga
1501 ggttgcagct agtaccagtg caggggttgg agtaaagagt gagccacagc caataataat
1561 cgggagacgc aatgaagttg tcattactga aaacaataac agcataactg agcaaatcac
1621 tgatgtggtg aagcaaccag cctttatagc tggtattggt ggtgcctgct gggtaattct
1681 gatgggtttt agcatatggt tgtattggcg aagaaagaag aggaagggac tcagtaatta
1741 tgctgttacg tttcaaagag gagatggagg actaatgagc aatggaagcc gtccaggtct
1801 tctcaatgct ggtgatccca gctatccatg gcttgctgat tcttggccag ccacgagctt
1861 gccagtaaat aatagcaaca gtggcccaaa tgagattgga aattttggcc gtggagatgt
1921 gctgccacca gttccaggcc aaggggataa aacagcaacg atgctctcag atggagccat
1981 ttatagtagc attgacttca ctaccaaaac cagttacaac agttccagcc aaataacaca
2041 ggctacccca tatgccacga cacagatctt gcattccaac agcatacatg aattggctgt
2101 cgatctgcct gatccacaat ggaaaagctc aattcagcaa aaaacagatc tgatgggatt
2161 tggttattct ctacctgatc agaacaaagg taacaatggt gggaaaggtg gaaaaaagaa
2221 gaaaaataaa aactcttcta aaccacagaa aaacaatgga tccacttggg ccaatgtccc
2281 tctacctccc cccccagtcc agccccttcc tggcacggag ctggaacact atgcagtgga
2341 acaacaagaa aatggctatg acagtgatag ctggtgccca ccattgccag tacaaactta
2401 cttacaccaa ggtctggaag atgaactgga agaagatgat gatagggtcc caacacctcc
2461 tgttcgaggc gtggcttctt ctcctgctat ctcctttgga cagcagtcca ctgcaactct
2521 tactccatcc ccacgggaag agatgcaacc catgctgcag gctcacctgg atgagttgac
2581 aagagcctat cagtttgata tagcaaaaca aacatggcac attcaaagca ataatcaacc
2641 tccacagcct ccagttccac cgttaggtta tgtgtctgga gccttgattt ctgatttgga
2701 aacggatgtt gcagatgatg atgccgacga cgaagaggaa gctttagaaa tccccaggcc
2761 cctgagagca ctggaccaga ctcctggatc cagcatggac aatctagaca gctctgtgac
2821 aggaaaagcc tttacctcct ctcaaagacc tcgacctacc agcccatttt ctactgacag
2881 taacaccagt gcagccctga gtcaaagtca gaggcctcgg cccactaaaa aacacaaggg
2941 agggcggatg gaccaacaac cagcattgcc tcatcgaagg gaaggaatga cagatgatct
3001 tccaccacca ccagatcccc cgccaggtca gggtttaagg cagcaaatag gcccgagcca
3061 gcaggctggt aacgtggaaa actcagcaga gagaaaagga agctctctag agagacaaca
3121 tgcatccagc ttagaagaca caaagagctc attggattgt ccagctagaa cctccctaga
3181 gtggcagcga caaacccagg aatggataag ctccacagaa cgacaagaag atatacggaa
3241 agccccacac aaacaaggtg tcggatcaga ggaggccttg gtgccctata gcaagcccag
3301 tttcccatct ccaggtggcc acagctcatc aggaacagct tcttctaagg gatccactgg
3361 acctaggaaa accgaggtgt tgagagcagg ccaccagcgc aatgccagcg accttcttga
3421 cataggatat atgggctcca acagtcaagg acagtttaca ggtgaattat gtacagacta
3481 caaagaccat gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa
3541 gtaactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
3601 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
3661 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt agttcttgcc
3721 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
3781 actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
3841 atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
3901 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
3961 gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga gcggccgcag
4021 gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc
4081 gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga
4141 gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt
4201 atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg
4261 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg
4321 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
4381 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
4441 aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
4501 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
4561 tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggtctatt
4621 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt
4681 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
4741 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
4801 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
4861 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
4921 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
4981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
5041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
5101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
5161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
5221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
5281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
5341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
5401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
5461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
5521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
5581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
5641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
5701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
5761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
5821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
5881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
5941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
6001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
6061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
6121 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
6181 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
6241 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
6301 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
6361 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
6421 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
6481 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
6541 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
6601 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
6661 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
6721 ggttcctggc cttttgctgg ccttttgctc acatgt
//
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