| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001375 |
| 产品用途: | 3' AAVLINK plasmid for RERE expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RERE |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-RERE-FLAG 6215 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6215)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6215
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1480..1485
/label=SDS
CDS 1482..2950
/codon_start=1
/label=RERE
/translation="EAA*RGGRPAHSPPSRSRRRLWTTLRSLRAPLPHQGARPRSPLWW
TPPVTPASQLGSTNTWTGATTRVPGQTCTSCLWPGPSWPRRGRRPLRRPSARLSRKPER
SESGRRRRRRSGSGSESGSARQSGRLRRPAQRMKVASVTHSSVVLATCGHPSSHHQPPL
LLCPPTSGPTHLPFGL*ASTPGPTSCRPPTATTPSTCPLTPRTPCWPTTCLASTTSTPP
SASGSSGSGRSESGRSESGSCGRG*SRASR*SPQSWTPCTQPPTPWSTLPGTAPSPSPR
PPGPTLLLLSTRA*TPWRGRDWPWRAPSCGPR*ATLTDWQPSVSTQSAWHR*PAIPWPD
CRCST*LRTITSTLTFTPTSTSTSRTPSTKVQQAPFTRWSTP*LPVPTWLASPTRLALS
PTLCLDSPHTSTRCFATQFSAPPTPVTCLGPSHPPCQQPTSCRPCMPSRPSCRDWPWSS
SGCMDTPTCMVATYQVRKIITVD*RKKVTSS"
misc_feature 1533..1538
/label=SDS
misc_feature 1536..1541
/label=SDS
misc_feature 1743..1748
/label=SDS
CDS 2957..3022
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3026..3457
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3170..3175
/label=SDS
repeat_region 3500..3618
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3500..3607
/label=AAV2 ITR
rep_origin 3693..4148
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4430..4534
/gene="bla"
/label=AmpR promoter
CDS 4535..5395
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5566..6154
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggaggcagca tagcgggggg
1501 gtcgtcctgc ccactcccca ccgtccagat caaggaggag gctctggacg acgctgagga
1561 gcctgagagc ccccctcccc caccaaggag cccgtccccg gagcccactg tggtggacac
1621 ccccagtcac gccagccagt cagctaggtt ctacaaacac ctggaccggg gctacaactc
1681 gtgtgcccgg acagacctgt acttcatgcc tctggccggg tccaagctgg ccaagaagag
1741 ggaggaggcc attgagaagg ccaagcgcga ggctgagcag aaagcccgag aggagcgaga
1801 gcgggagaag gagaaggaga aggagcggga gcgggagcga gagcgggagc gcgaggcaga
1861 gcgggcggct aaggcgtcca gctcagcgca tgaaggtcgc ctcagtgacc cacagctcag
1921 tggtcctggc cacatgcggc catccttcga gccaccacca accaccattg ctgctgtgcc
1981 cccctacatc gggcccgaca cacctgccct tcggactctg agcgagtacg cccggcccca
2041 cgtcatgtcg cccaccaacc gcaaccaccc cttctacatg ccccttaacc ccacggaccc
2101 cctgctggcc taccacatgc ctggcctcta caacgtcgac cccaccatcc gcgagcggga
2161 gctccgggag cgggagatcc gagagcggga gatccgagag cgggagctgc gggagaggat
2221 gaagccgggc ttcgaggtga agcccccaga gctggacccc ctgcacccag ccgccaaccc
2281 catggagcac tttgcccggc acagcgccct caccatcccc ccgaccgccg ggccccaccc
2341 ttttgcttct ttccacccgg gcctgaaccc cttggagagg gagagactgg ccctggcggg
2401 cccccagctg cggcccgaga tgagctaccc tgacagactg gcagccgagc gtatccacgc
2461 agagcgcatg gcatcgctga ccagcgatcc cctggcccga ctgcagatgt tcaacgtgac
2521 tccgcaccat caccagcact ctcacattca ctcccacctc cacctccacc agcaggaccc
2581 cctccaccaa ggttcagcag gccccgttca cccgctggtc gaccccctga ctgccggtcc
2641 ccacctggct cgcttcccct acccgcctgg cactctcccc aaccctctgc ttggacagcc
2701 cccacacgag cacgagatgc ttcgccaccc agttttcggc accccctacc cccgtgacct
2761 gcctggggcc atcccacccc ccatgtcagc agcccaccag ctgcaggcca tgcatgccca
2821 gtcggccgag ctgcagagac tggccatgga gcagcagtgg ctgcatggac acccccacat
2881 gcatggtggc cacctaccaa gtcaggaaga ttattacagt cgactgaaga aagaaggtga
2941 caagcagtta tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
3001 ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt
3061 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
3121 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
3181 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
3241 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
3301 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
3361 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
3421 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
3481 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
3541 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc ggcctcagtg agcgagcgag
3601 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
3661 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
3721 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
3781 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
3841 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
3901 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
3961 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4021 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4081 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4141 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4201 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4261 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4321 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4381 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4501 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4561 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4621 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4681 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4741 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
4801 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
4861 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
4921 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
4981 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5041 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5101 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5161 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5221 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5281 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5341 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5401 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5461 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5521 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5581 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5641 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5701 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
5761 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
5821 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
5881 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
5941 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6001 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6061 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6121 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6181 gttcctggcc ttttgctggc cttttgctca catgt
//
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