| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001360 |
| 产品用途: | 3' AAVLINK plasmid for RALGAPB expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | RALGAPB |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-RALGAPB-FLAG 5739 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5739)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5739
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..2452
/codon_start=1
/label=RALGAPB
/translation="VRKRTKRF*RMWSLPELFSHIS*NFCFPLAGQ*MWADTLVGLGMF
LPVGLLIVVMMVKDLNKK*FPLKILELAFSMDRRRCCIMLMPLQKLLLWFLLLWSP*LI
HWKVTSRTKIVIQIWILCQEF*NSHP*HLSFSPIIQTILIPHRGSVPVPE*GSCLRVAL
FLPLDLRQEFL*SGWNAMMI*KTFPSQS**QRSVLVWKLLQIVALH*DLQLLKKKFLSS
SSTL*TLDYSG*KFKEPLENLIWSSLLWMG*LSAGELLAFW*GRL*LTFVEERDWKVTP
TVPPMSAGNRKSPTLSTSTGTSSWSQSFILHFSRRLDSRTAV"
misc_feature 2424..2429
/label=SDS
CDS 2459..2524
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 2528..2959
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 2672..2677
/label=SDS
repeat_region 3002..3142
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3002..3131
/label=AAV2 ITR
rep_origin 3217..3672
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3954..4058
/gene="bla"
/label=AmpR promoter
CDS 4059..4919
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5090..5678
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtcagaaaa cgaaccaaga
1501 gattttaaag aatgtggagt cttccagaac tgttcagcca catttcctag aatttttgct
1561 ttcccttggc tggtcagtag atgtgggcag acaccctggt tggactgggc atgtttctac
1621 cagttggtct attaattgtt gtgatgatgg tgaaggatct caacaagaag tgatttcctc
1681 tgaagatatt ggagctagca ttttcaatgg acagaagaag gtgctgtatt atgctgatgc
1741 ccttacagaa attgcttttg tggttccttc tcctgtggag tccttaactg attcattgga
1801 aagtaacatc tcggaccaag atagtgattc aaatatggat cttatgccag gaattctgaa
1861 acagccatcc ctgacacttg agcttttccc caatcataca gacaatctta attcctcaca
1921 gaggctcagt cccagttcca gaatgaggaa gctgcctcag ggtcgccctg ttcctcccct
1981 tggacctgag acaagagttt ctgtagtctg ggtggaacgc tatgatgata tagaaaactt
2041 tcccctctca gagctgatga cagagatcag tactggtgtg gaaactactg caaatagtag
2101 cacttcactg agatctacaa ctcttgaaaa agaagttcct gtcatcttca tccacccttt
2161 aaacactgga ttattccgga taaaaattca aggagccact ggaaaattta atatggtcat
2221 ccctcttgtg gatgggatga ttgtcagcag gcgagctctt ggctttctgg tgaggcagac
2281 tgtaattaac atttgtagaa gaaagagact ggaaagtgac tcctacagtc ccccccatgt
2341 ccgccggaaa cagaaaatca ccgacattgt caacaagtac cggaacaagc agctggagcc
2401 agagttttat acttcacttt tccaggaggt tggactcaag aactgcagtt cttgtacaga
2461 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
2521 caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
2581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
2641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt
2701 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
2761 ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta
2821 accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
2881 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
2941 gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg
3001 caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag
3061 gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag
3121 cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc
3181 ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa
3241 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc
3301 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
3361 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
3421 aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
3481 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
3541 cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct
3601 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
3661 cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
3721 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
3781 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
3841 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
4741 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
5221 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
5701 tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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