| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001477 |
| 产品用途: | 3' AAVLINK plasmid for PXDN expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PXDN |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PXDN-FLAG 6403 bp DNA circular SYN 14-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6403)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6403
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3104
/codon_start=1
/label=PXDN
/translation="GIVQRSGKPLLPFATGPPTECMRDENESPIPCFLAGDHRANEQLG
LTSMHTLWFREHNRIATELLKLNPHWDGDTIYYETRKIVGAEIQHITYQHWLPKILGEV
GMRTLGEYHGYDPGINAGIFNAFATAAFRFGHTLVNPLLYRLDENFQPIAQDHLPLHKA
FFSPFRIVNEGGIDPLLRGLFGVAGKMRVPSQLLNTELTERLFSMAHTVALDLAAINIQ
RGRDHGIPPYHDYRVYCNLSAAHTFEDLKNEIKNPEIREKLKRLYGSTLNIDLFPALVV
EDLVPGSRLGPTLMCLLSTQFKRLRDGDRLWYENPGVFSPAQLTQIKQTSLARILCDNA
DNITRVQSDVFRVAEFPHGYGSCDEIPRVDLRVWQDCCEDCRTRGQFNAFSYHFRGRRS
LEFSYQEDKPTKKTRPRKIPSVGRQGEHLSNSTSAFSTRSDASGTNDFREFVLEMQKTI
TDLRTQIKKLESRLSTTECVDAGGESHANNTKWKKDACTICECKDGQVTCFVEACPPAT
CAVPVNIPGACCPVCLQKRAEEKP"
misc_feature 2695..2700
/label=SDS
CDS 3111..3176
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3192..3623
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3336..3341
/label=SDS
repeat_region 3666..3806
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3666..3795
/label=AAV2 ITR
rep_origin 3881..4336
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4618..4722
/gene="bla"
/label=AmpR promoter
CDS 4723..5583
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5754..6342
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcatcgtg cagcggtccg
1501 ggaagccgct gctccccttc gccaccgggc cgcccacgga gtgcatgcgg gacgagaacg
1561 agagccccat cccctgcttc ctggccgggg accaccgcgc caacgagcag ctgggcctga
1621 ccagcatgca cacgctgtgg ttccgcgagc acaaccgcat tgccacggag ctgctcaagc
1681 tgaacccgca ctgggacggc gacaccatct actatgagac caggaagatc gtgggtgcgg
1741 agatccagca catcacctac cagcactggc tcccgaagat cctgggggag gtgggcatga
1801 ggacgctggg agagtaccac ggctacgacc ccggcatcaa tgctggcatc ttcaacgcct
1861 tcgccaccgc ggccttcagg tttggccaca cgcttgtcaa cccactgctt taccggctgg
1921 acgagaactt ccagcccatt gcacaagatc acctccccct tcacaaagct ttcttctctc
1981 ccttccggat tgtgaatgag ggcggcatcg atccgcttct cagggggctg ttcggggtgg
2041 cggggaaaat gcgtgtgccc tcgcagctgc tgaacacgga gctcacggag cggctgttct
2101 ccatggcaca cacggtggct ctggacctgg cggccatcaa catccagcgg ggccgggacc
2161 acgggatccc accctaccac gactacaggg tctactgcaa tctatcggcg gcacacacgt
2221 tcgaggacct gaaaaatgag attaaaaacc ctgagatccg ggagaaactg aaaaggttgt
2281 atggctcgac actcaacatc gacctgtttc cggcgctcgt ggtggaggac ctggtgcctg
2341 gcagccggct gggccccacc ctgatgtgtc ttctcagcac acagttcaag cgcctgcgag
2401 atggggacag gttgtggtat gagaaccctg gggtgttctc cccggcccag ctgactcaga
2461 tcaagcagac gtcgctggcc aggatcctat gcgacaacgc ggacaacatc acccgggtgc
2521 agagcgacgt gttcagggtg gcggagttcc ctcacggcta cggcagctgt gacgagatcc
2581 ccagggtgga cctccgggtg tggcaggact gctgtgaaga ctgtaggacc agggggcagt
2641 tcaatgcctt ttcctatcat ttccgaggca gacggtctct tgagttcagc taccaggagg
2701 acaagccgac caagaaaaca agaccacgga aaatacccag tgttgggaga cagggggaac
2761 atctcagcaa cagcacctca gccttcagca cacgctcaga tgcatctggg acaaatgact
2821 tcagagagtt tgttctggaa atgcagaaga ccatcacaga cctcagaaca cagataaaga
2881 aacttgaatc acggctcagt accacagagt gcgtggatgc cgggggcgaa tctcacgcca
2941 acaacaccaa gtggaaaaaa gatgcatgca ccatttgtga atgcaaagac gggcaggtca
3001 cctgcttcgt ggaagcttgc ccccctgcca cctgtgctgt ccccgtgaac atcccagggg
3061 cctgctgtcc agtctgctta cagaagaggg cggaggaaaa gccctgtaca gactacaaag
3121 accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat gacaagtaag
3181 aattctgatg actcgagata atcaacctct ggattacaaa atttgtgaaa gattgactgg
3241 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
3301 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
3361 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
3421 gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta ttgctttatt
3481 tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
3541 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
3601 gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg
3661 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
3721 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
3781 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
3841 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
3901 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta
3961 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
4021 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
4081 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
4141 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
4201 acaacactca actctatctc gggctattct tttgatttat aagggatttt gccgatttcg
4261 gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
4321 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
4381 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
4441 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
4501 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
4561 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
4621 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
4681 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4741 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4801 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4861 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4921 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4981 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
5041 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
5101 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
5161 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
5221 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
5281 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
5341 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
5401 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
5461 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
5521 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
5581 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
5641 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
5701 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5761 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5821 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5881 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
5941 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
6001 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
6061 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
6121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
6181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
6241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
6301 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
6361 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
