| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001432 |
| 产品用途: | 3' AAVLINK plasmid for PRR14L expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PRR14L |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PRR14L-FLAG 7205 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7205)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7205
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3906
/codon_start=1
/label=PRR14L
/translation="GTTGLLNCWGAI*GVGYQRNWRWRSREQHLSDPF*MPGDT*SC*K
TAEP*GFGLHVAERREIYTSTKSTYDIATVHII*SVVR*CTKSEPTEG*QR*VHHDQ*N
HPSKAGQGQHCGTDSEVRRPEGGKVTSSIKEGH*VMHKSLPSWCPSESTRPLLCWV*SN
TWCLCEERSSSLKEAAPSNL*ESFLSGANHCWEKNR*NQKFCLFKEFFQSHPHKSAQTS
QFVYSVCTYTIRT*NSTYQELG*SHTKADVYTVPSLEEPEF*EDYQRISLTKQAVHPCL
QTGPSHEDSEAKIPTVFL*TSSNG*KLQAPQI*KTPGRIFIQHRAAESIFGS*WMG*EA
*Q*AHGTLFSRIHQDDLHRFEQQDAVPAVWF*NLPSILSCEIIQLRLHD*VLKDFS*AL
CSGKACLRRGPPVPVSTSQVDLFFLLVPRLPWDGNIQGRHWRP*SDPHSGSSPASSSSP
RLWRHCHSPDQSRLLCPWPSHTSSTLFPRMLPNLDKKTELLQPHAYHAEALHDPVYTGP
ERVTVSSLHSRQGLLFSALLGGQSPIHLEPAWSFCLLLRNLFSSFPSLQAATKSGHHKQ
PHHVTICASSRHGSYI*HQRQSDEAGASIPCLGTKVLLGSRISCQQAPAFSL*VPGSWI
G*AGWCESSMPLPTEQPPRTERG*AREEAKESLTDSHPENHS*ARS*SYPHGPSSTQKV
KEEGV*FRRDIYQQEL*ISSCKQVFRDHL*GTQGTKWYTNLNQPTEEEASSRISGFYSP
AKEESSRQSQGGRQLYQGPEGSCAESRAGCSFDTETNGTGDLLCQGRGAGTIIRL"
misc_feature 1856..1861
/label=SDS
misc_feature 1946..1951
/label=SDS
misc_feature 3619..3624
/label=SDS
CDS 3913..3978
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3994..4425
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4138..4143
/label=SDS
repeat_region 4468..4608
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4468..4597
/label=AAV2 ITR
rep_origin 4683..5138
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5420..5524
/gene="bla"
/label=AmpR promoter
CDS 5525..6385
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6556..7144
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggacgact gggttactta
1501 actgttgggg agcaatctga ggagttggtt accagagaaa ctggcgatgg cgatcccgtg
1561 agcaacatct ctcagaccca ttttaaatgc cgggggatac ttaatcatgc tgaaaaacag
1621 cagagccctg aggttttgga ctacatgttg cagaaagaag agaaatatat acgtcaacaa
1681 aaagcacata cgatatcgca acagtgcata tcatctagtc tgttgttaga tgatgcacaa
1741 aatcagaacc aaccgaaggc tgacaaagat gagtccacca tgatcaatga aatcacccta
1801 gcaaagctgg ccaaggacag cattgtggca cagactcaga agttagaaga ccagaaggag
1861 gaaaggttac atcatccatt aaggaaggac actgagtcat gcacaagtcc ttgccttctt
1921 ggtgcccctc ggaaagcaca agacccctcc tctgctgggt gtgatcaaat acatggtgcc
1981 tttgcgaaga aaggagttct tcccttaaag aagcagcccc atcgaacttg taagaaagtt
2041 tcctatcagg agcaaatcat tgttgggaga aaaataggta aaatcagaag ttctgccttt
2101 ttaaagagtt cttccaatcc catccccaca aaagcgcaca gacttctcag tttgtgtact
2161 ctgtctgcac ctacacgatt agaacctgaa acagcaccta ccaagagctt ggttagtcac
2221 ataccaaagc agatgtctac accgtgccat cccttgagga gcctgaattt taggaagact
2281 accaaagaat cagccttact aaacaagctg tccatccttg cctccaaact ggccccagcc
2341 atgaagactc agaagctaag ataccgacgg tgttcctctg aacttcttcc aatggctaaa
2401 agctacaagc gcctcagata taaaagactc ctggacggat tttcatccag cacagagcag
2461 ctgaatccat atttggcagc tagtggatgg gataagaggc ctaacagtaa gcccatggca
2521 ctttattctc tcgaatccat caagatgacc ttcatagatt tgagcaacaa gatgccgtcc
2581 ctgctgtttg gttctgaaat cttcccagta tcctttcatg tgaaatcatc cagctcagat
2641 tgcacgactg agtcctcaag gacttttcct gagcactgtg ctccggcaag gcttgcctta
2701 ggagaggccc tccagtgccc gtctcaacct cccaagtgga ccttttcttt cttcttgtcc
2761 cacggttgcc ctgggatggc aacattcagg gaagacactg gcgtccatag tcagacccac
2821 actcaggctc ctccccagcc tccagctcct ctccaagact atggaggcac tgccatagtc
2881 cagaccagag cagactgctc tgtccttggc cttcacacac ttctagcact ttgttcccca
2941 ggatgttacc gaatctggac aaaaaaacgg agcttctcca gccacatgcc taccatgcag
3001 aggctcttca tgacccagtt tacacagggc ctgaaagggt tacggtctcc agcctccata
3061 gcagacaagg tcttctgttc tctgccctac tcggtgggca gagtcctatc catttggagc
3121 cagcatggtc cttctgtctg ctccttcgaa atctcttctc ttcattcccc tcactgcaag
3181 cggcaaccaa gtctgggcac cacaagcagc cacaccatgt taccatatgt gcctcttcca
3241 ggcatggaag ctacatataa caccagcggc agtcagacga ggctggagcc tccattccct
3301 gccttggtac caaagtcttg cttggtagca gaatcagctg tcagcaagct cctgctttca
3361 gcctctgagt tccaggttcg tggattggat gagctggatg gtgtgaaagc agcatgcccc
3421 tgcccacaga gcagcccccc agaacagaaa gaggctgagc cagagaagag gccaaagaaa
3481 gtctcacaga ttcgcatccg gaaaaccatt cctaggccag atcctaatct tacccccatg
3541 ggccttcctc gacccaaaag gttaaagaag aaggagttta gtttagaaga gatatatacc
3601 aacaagaatt ataaatctcc tcctgcaaac aggtgtttag agaccatctt tgaggaaccc
3661 aaggaacgaa atggtacact aatctcaatc agccaacaga agaggaagcg agttctagaa
3721 tttcaggatt ttacagtccc gcgaaagagg agagctcgag gcaaagtcaa ggtggcaggc
3781 agctttacca gggcccagaa ggcagctgtg cagagtcgag agctggatgc tcttttgata
3841 cagaaactaa tggaactgga gaccttcttt gccaaggaag aggagcagga acaatcatca
3901 ggctgttgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
3961 aaggatgacg atgacaagta agaattctga tgactcgaga taatcaacct ctggattaca
4021 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
4081 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
4141 ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc
4201 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa
4261 tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct
4321 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
4381 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg
4441 tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct
4501 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
4561 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc
4621 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata
4681 gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
4741 cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
4801 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
4861 tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg
4921 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
4981 tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt
5041 ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt
5101 taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat
5161 ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc
5221 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
5281 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt
5341 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
5401 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
5461 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
5521 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
5581 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
5641 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
5701 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
5761 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
5821 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
5881 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
5941 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
6001 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
6061 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
6121 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
6181 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
6241 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
6301 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
6361 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
6421 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
6481 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
6541 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
6601 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
6661 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
6721 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
6781 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
6841 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
6901 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
6961 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
7021 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
7081 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
7141 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
7201 catgt
//
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