| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001429 |
| 产品用途: | 3' AAVLINK plasmid for PRR12 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PRR12 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PRR12-FLAG 7245 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7245)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..7245
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3946
/codon_start=1
/label=PRR12
/translation="GAPGWSP*SHLRSSCLCPRLARVWEPHRVMPYQALTTTAWTRA*L
GRRSRPRLRRWRRSSRR*SRVSWPPSWTSSSQASATHHSTRRA*RLRSALPRVCHPLCQ
PEACSPSPLPPLLCHIPHLPEPLGLGAPWRLQRVRVWGLAALHPASGLMRS*SGTSRRC
PPSPRMRKTLSPRTETCRRASPPPSLPSMTHPLLGQKTLPPQMGRPWPPRLQFQGHPLF
RGSPVPTAMALPSPRCWRRNPHPLHLLPRLLSLSLRHPLRRHSQPCPRHPRWWPPRPAH
HRHRRCRRHLHQPCPRLHHHPHQPLPHWLLLLRSPPPRLPKTPSCRTPGPCIWPKSRRR
RQCVGRRTRRPARVAERASSGNGTSSSSVLRTSLPSSWRCRRGVNPHPSGESRRPFCRN
SLRRSRTARGSFVPPVIIWGILGMQKIGTSASM*SSWKMSIRRTT*GSVLGNPGIGPQC
QSDALGRPRTPYLLGVALHLPLRPQHHLPSLRPLKRRHLRSPQSRLLRRPCLSPLPPRS
PPSCGLLRRKRRRRR*HVESGHCGVSGPPADGRHGQSGVSPRDNLPHPGCPKPGLPR*R
LNRPLRRGRNG*RRQAATLQQAGAHQAAPRTRSPPLEPPARTSGQYLGVCSKPGRCGRC
TGATWRCW*AQHLTQT*SRPWRTRMTSCTCPPCGR*TAC*MSTRRKS*SGCR*AQPCRM
LCTRSHSCKWSRVGRALRKRGLCGCGLLGNPTTARRSASSRGAWSEPRSSRLSWKSRDT
IHSTIRSTTINTTPSCAAGTRPWPSRAAPRTWARRRWSSSACGTSRGWNSSLTPSVTCW
PKHRPTAAA"
RBS 1639..1647
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
misc_feature 1641..1646
/label=SDS
misc_feature 1686..1691
/label=SDS
misc_feature 1971..1976
/label=SDS
CDS complement(2045..2056)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
misc_feature 2222..2227
/label=SDS
misc_feature 2414..2419
/label=SDS
misc_feature 2526..2531
/label=SDS
misc_feature 3037..3042
/label=SDS
misc_feature 3246..3251
/label=SDS
misc_feature 3840..3845
/label=SDS
misc_feature 3843..3848
/label=SDS
CDS 3953..4018
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4034..4465
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4178..4183
/label=SDS
repeat_region 4508..4648
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4508..4637
/label=AAV2 ITR
rep_origin 4723..5178
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5460..5564
/gene="bla"
/label=AmpR promoter
CDS 5565..6425
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6596..7184
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggggcaccc ggttggagcc
1501 cctgaagcca cttaagatca agctgtctgt gcccaaggct ggcgagggtc tgggaacctc
1561 atcgggtgat gccatatcag gcactgacca caacagcctg gactcgagcc tgactcggga
1621 gaagatcgag gccaagatta aggaggtgga ggagaagcag ccggagatga agtcgggttt
1681 catggcctcc ttcttggact tcctcaagtc aggcaagcgc cacccaccac tctaccaggc
1741 gggcctgacg cctccgctca gccctcccaa gagtgtgcca ccctctgtgc cagcccgagg
1801 cctgcagccc cagccccctg ccacccctgc tgtgccacat cccccacctt ccggagcctt
1861 tgggcttggg ggcgccctgg aggctgcaga gagtgagggt ctggggcttg gctgcccttc
1921 accctgcaag cggcttgatg aggagctgaa gcggaacctc gagacgctgc cctccttctc
1981 ctcggatgag gaagactctg tcgccaagaa ccgagacctg caggagagca tctcctccgc
2041 catctctgcc ctcgatgacc caccccttgc tgggccaaaa gacacttcca ccccagatgg
2101 gccgcccttg gcccccgcgg ctgcagttcc agggccaccc cctcttccgg ggctccccag
2161 tgccaacagc aatggcactc ccgagccccc gctgctggag gagaaacccc cacccactcc
2221 acctcctgcc ccgactcctc agcctcagcc tccgccaccc cctccgccgc cacagccagc
2281 cctgccctcg ccacccccgc tggtggcccc cacgcccagc tcaccaccgc caccgccgct
2341 gccgccgcca cctccaccag ccatgccctc gcctccacca ccacccccac cagccgctgc
2401 cccactggct gctcctcctg aggagcccgc cgccccgtct cccgaagacc ccgagctgcc
2461 ggacacccgg cccctgcatc tggccaaaaa gcaggagacg gcggcagtgt gtggggagac
2521 ggacgaggag gccggcgaga gtggcggaga gggcatcttc cgggaacggg acgagttcgt
2581 catccgtgct gaggacatcc cttccctcaa gctggcgttg cagacggggc gtgaaccccc
2641 acccatctgg cgagtccaga aggcccttct gcagaaattc actccggaga tcaaggacgg
2701 ccagaggcag ttttgtgcca ccagtaatta tttggggtat tttggggatg caaaaaatcg
2761 gtaccagcgc ctctatgtaa agttcctgga aaatgtcaat aagaaggact acgtgagggt
2821 ctgtgctcgg aaaccctggc atcggccccc agtgccagtc agacgctctg ggcaggccaa
2881 gaaccccgta tctgctgggg gtagctctgc acctccccct aaggccccag caccacctcc
2941 caagcctgag acccctgaaa agacgacatc tgagaagccc ccagagcaga ctcctgagac
3001 ggccatgcct gagccccctg cccccgagaa gccctccctc ctgcggcctg ttgagaagga
3061 aaaggagaag gagaaggtga cacgtggaga gcggccattg cggggtgagc gggccaccag
3121 cggacggcag acacggccag agcggagtct cgccacggga caacctgcca catcccggct
3181 gcccaaagcc cggcctacca aggtgaaggc tgaaccgccc cctaagaaga ggaagaaatg
3241 gctgaaggag gcaggcggca acgctacagc aggcgggggc ccaccaggca gctcctcgga
3301 ctcggagtcc tcccctggag cccccagcga ggacgagcgg gcagtacctg ggcgtctgct
3361 caaaaccagg gcgatgcggg agatgtaccg gagctacgtg gagatgttgg tgagcacagc
3421 acttgaccca gacatgatcc aggccctgga ggacacgcat gacgagctgt acctgccccc
3481 catgcggaag atagacggcc tgctgaatga gcacaagaag aaagtcctga agcggctgtc
3541 gctaagccca gccctgcagg atgctctgca cacgttccca cagctgcaag tggagcagag
3601 tggggagggc tctccggaag agggggctgt gcggctgcgg cctgctgggg aaccctacaa
3661 ccgcaagacg ctcagcaagc tcaagaggag cgtggtcaga gcccaggagt tcaaggttga
3721 gctggaaaag tcgggatact atacactcta ccattcgctc caccactata aataccacac
3781 cttcctgcgc tgccgggacc agaccctggc catcgagggc ggcgccgagg acctgggcca
3841 ggaggaggtg gtccagcagt gcatgcggaa ccagccgtgg ctggaacagc tctttgactc
3901 cttcagtgac ctgctggccc aagcacaggc ccacagccgc tgcgggtgta cagactacaa
3961 agaccatgac ggtgattata aagatcatga catcgactac aaggatgacg atgacaagta
4021 agaattctga tgactcgaga taatcaacct ctggattaca aaatttgtga aagattgact
4081 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
4141 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta
4201 gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
4261 ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta
4321 tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
4381 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
4441 gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag
4501 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
4561 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
4621 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
4681 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
4741 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
4801 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
4861 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
4921 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
4981 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
5041 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
5101 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
5161 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
5221 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
5281 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
5341 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
5401 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
5461 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
5521 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
5581 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
5641 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
5701 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
5761 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
5821 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
5881 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
5941 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
6001 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
6061 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
6121 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
6181 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
6241 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
6301 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
6361 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
6421 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
6481 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
6541 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
6601 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
6661 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
6721 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
6781 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
6841 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
6901 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
6961 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
7021 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
7081 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
7141 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
7201 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
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