| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001408 |
| 产品用途: | 3' AAVLINK plasmid for PHIP expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | PHIP |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-PHIP-FLAG 7013 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7013)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 6, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7013
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3726
/codon_start=1
/label=PHIP
/translation="VCHR*CLVVWNNRKPGTSST*VP**SVSMLQCLLGQWRYRKDESL
GYGAYT**CCIS*RTRYQCSFN*W*VQITNL*TS*WRMGYQSQG*RM*KNCGRNKPVDD
TRYCLSICGPRGSASLSHVLHSSGISNGSKYN*TKTGKQVLQAGFFPNVGSSIYRA*YT
NI**AWKPYCEIC*IRD*SSSTFYKGSDLL*HNSTL*FNEEESFV*F*G*RERC*CARN
FYSKKEGPSA*KKIT**SPVLRYSSMEETV*RIVKSHISM*RFRAFPSAGRSP*ISRLQ
RHH*HSNGFCYR*RNFRGWEL*VTNGVM*RCQTYFQ*FQSIYTKQKIKDLQHEFALVCI
L*RTH*FSFIRL*ICSSFS*KKYHNQKEEEKKQKQLCFQ*CCIKP*KEKKDLKTPAKIR
KLYLCILYTYTINTAKTQCCSDKR*NRI*FCGSNQKQPSGCRSSCH*ATIYFFSCKDFY
YKS*CICNTRENNTREFCETFQSFEYSFQSWSIQF*SWH*E*FCKRKHGKGKASQT*NE
VICTPKGVHSFKVISCH*ARRL*EQRSCTRNHSSKWPWRTAIKTCEEGTWKET*SRS*Y
Q*W*NYTQEKG*KAQKATVCKARRFRAK*CASHQR*STSFFNMQFSF*N**CKGRFVTE
KESWR*EAQKEDEDTKIRCRSPSPCKCQSVKEK*PKKDR*SYR*GRRV*RTQRL*TPHE
N*KSRSKDSFL**G*L*RGAKAAVVRRHLFNFWNF**RTSPKVD*KSKS*FNWL"
misc_feature 2581..2586
/label=SDS
CDS 3733..3798
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3802..4233
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3946..3951
/label=SDS
repeat_region 4276..4416
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4276..4405
/label=AAV2 ITR
rep_origin 4491..4946
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5228..5332
/gene="bla"
/label=AmpR promoter
CDS 5333..6193
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6364..6952
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtctgtcat agatgatgcc
1501 tggtggtttg gaacaatcga aagccaggaa cctcttcaac ctgagtaccc tgatagtctg
1561 tttcaatgct acaatgtttg ctgggacaat ggagatacag aaaagatgag tccttgggat
1621 atggagctta tacctaataa tgctgtattt cctgaagaac taggtaccag tgttccttta
1681 actgatggtg agtgcagatc actaatctat aaacctcttg atggagaatg gggtaccaat
1741 cccagggatg aagaatgtga aagaattgtg gcaggaataa accagttgat gacactagat
1801 attgcctcag catttgtggc ccccgtggat ctgcaagcct atcccatgta ttgcacagta
1861 gtggcatatc caacggatct aagtacaatt aaacaaagac tggaaaacag gttttacagg
1921 cgggtttctt ccctaatgtg ggaagttcga tatatagagc ataatacacg aacatttaat
1981 gagcctggaa gccctattgt gaaatctgct aaattcgtga ctgatcttct tctacatttt
2041 ataaaggatc agacttgtta taacataatt ccactttata attcaatgaa gaagaaagtt
2101 ttgtctgatt ctgaggatga agagaaagat gctgatgtgc caggaacttc tactcgaaaa
2161 aggaaggacc atcagcctag aagaagatta cgtaatagag cccagtctta cgatattcaa
2221 gcatggaaga aacagtgtga agaattgtta aatctcatat ttcaatgtga agattcagag
2281 cctttccgtc agccggtaga tctccttgaa tatccagact acagagacat cattgacact
2341 ccaatggatt ttgctaccgt tagagaaact ttagaggctg ggaattatga gtcaccaatg
2401 gagttatgta aagatgtcag acttattttc agtaattcca aagcatatac accaagcaaa
2461 agatcaagga tttacagcat gagtttgcgc ttgtctgcat tctttgaaga acacattagt
2521 tcagttttat cagattataa atctgctctt cgttttcata aaagaaatac cataaccaaa
2581 aggaggaaga aaagaaacag aagcagctct gtttccagta gtgctgcatc aagccctgaa
2641 aggaaaaaaa ggatcttaaa accccagcta aaatcagaaa gctctacctc tgcattctct
2701 acacctacac gatcaatacc gccaagacac aatgctgctc agataaacgg taaaacagaa
2761 tctagttctg tggttcgaac cagaagcaac cgagtggttg tagatccagt tgtcactgag
2821 caaccatcta cttcttcagc tgcaaagact tttattacaa aagctaatgc atctgcaata
2881 ccagggaaaa caatactaga gaattctgtg aaacattcca aagctttgaa tactctttcc
2941 agtcctggtc aatccagttt tagtcatggc actaggaata attctgcaaa agaaaacatg
3001 gaaaaggaaa agccagtcaa acgtaaaatg aagtcatctg tactcccaaa ggcgtccact
3061 ctttcaaagt catcagctgt cattgagcaa ggagattgta agaacaacgc tcttgtacca
3121 ggaaccattc aagtaaatgg ccatggagga cagccatcaa aacttgtgaa gaggggacct
3181 ggaaggaaac ctaaagtaga agttaatacc aatagtggtg aaattataca caagaaaagg
3241 ggtagaaagc ccaaaaagct acagtatgca aagccagaag atttagagca aaataatgtg
3301 catcccatca gagatgaagt acttccttct tcaacatgca attttctttc tgaaactaat
3361 aatgtaaagg aagatttgtt acagaaaaag aatcgtggag gtaggaagcc caaaaggaag
3421 atgaagacac aaaaattaga tgcagatctc ctagtccctg caagtgtcaa agtgttaagg
3481 agaagtaacc gaaaaaagat agatgatcct atagatgagg aagaagagtt tgaagaactc
3541 aaaggctctg aaccccacat gagaactaga aatcaaggtc gaaggacagc tttctataat
3601 gaggatgact ctgaagagga gcaaaggcag ctgttgttcg aagacacctc tttaactttt
3661 ggaacttcta gtagaggacg agtccgaaag ttgactgaaa aagcaaaagc taatttaatt
3721 ggttggtgta cagactacaa agaccatgac ggtgattata aagatcatga catcgactac
3781 aaggatgacg atgacaagta actcgagata atcaacctct ggattacaaa atttgtgaaa
3841 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
3901 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
3961 cctggttagt tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
4021 gggctcggct gttgggcact gacaattccg tggtgtttat ttgtgaaatt tgtgatgcta
4081 ttgctttatt tgtaaccatc tagctttatt tgtgaaattt gtgatgctat tgctttattt
4141 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
4201 caggttcagg gggagatgtg ggaggttttt taaagatctg gagaccggtg tggcacgtgc
4261 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4321 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4381 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4441 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4501 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4561 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4621 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4681 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4741 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4801 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
4861 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
4921 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
4981 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
5041 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
5101 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
5161 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
5221 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
5281 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
5341 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
5401 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5461 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5521 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5581 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5641 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5701 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5761 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
5821 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
5881 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
5941 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
6001 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
6061 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
6121 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
6181 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
6241 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
6301 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
6361 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6421 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6481 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6541 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6601 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6661 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6721 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6781 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
6841 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
6901 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
6961 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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