| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001459 |
| 产品用途: | 3' AAVLINK plasmid for NINL expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | NINL |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-NINL-FLAG 6324 bp DNA circular SYN 12-JAN-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6324)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 11, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6324
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3037
/codon_start=1
/label=NINL
/translation="VMAESLRRRQEPGLAAGKPRTQKLRRARPPPLPRHPTAPQRGGHA
CSPVEWMGILSQRSQSLSARAQRGWSSLEPGSCLCWEQRETPRKPSHGCGSHP*GRPLR
AGDRLRGYRPFRKSEHEAGAGAPRSRPRSSRPGPRAPWSLGVTSTVWRLPGEGPCHPTS
SSQTRRVPGRSSLLPQKRGRPK*RWRERRMTWKPNFYIWKTSSGLWRNM*I*ERTTDWS
SIDFLKKTLC*KTIWEGFGKSLKLQKVLTMHRGRKLRF*RKTRKRPALRWRCSTDRIRT
TRINYPSSMSGFFNWDRRLLPTRPKTRSIV*PFRC*HRAWRRWFAVGSSRVTKSKNLEL
NLNA*IRNIRACSCHGQS*PRPLRKVKTRCRELT*G*GRPRPSTCRRSGWCPRTVWPSC
IACSAFRESRPGGAWMHSGKNMRNS*KPQKSGWKRRR*F*RIWKCSSKRKWIS*RSSLK
RTRSPTCC*RSCTWRTPTW*EHFRPPRRSSEAPRNKAASWKKKFALSTNSSVGLPPQPS
L"
misc_feature 1500..1505
/label=SDS
misc_feature 2373..2378
/label=SDS
misc_feature 2445..2450
/label=SDS
misc_feature 2637..2642
/label=SDS
misc_feature 2711..2716
/label=SDS
CDS 3044..3109
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3113..3544
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3257..3262
/label=SDS
repeat_region 3587..3727
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3587..3716
/label=AAV2 ITR
rep_origin 3802..4257
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4539..4643
/gene="bla"
/label=AmpR promoter
CDS 4644..5504
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5675..6263
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgatggca gagagtctga
1501 ggaggcggca ggagccgggc ctcgccgcag gcaagcccag gacacagaag ctacgcagag
1561 cccggccccc gcccctgccc cggcatccca cggcccctca gagaggtggt cacgcatgca
1621 gccctgtgga gtggatgggg atattgtccc aaaggagcca gagcctttcg gcgcgagcgc
1681 agcggggctg gagcagcctg gagcccggga gctgcctctg ctgggaacag agagagacgc
1741 ctcgcaaacc cagccacgga tgtgggagcc acccctgagg ccggccgctt cgtgcagggg
1801 acaggctgag aggctacagg ccattcagga agagcgagca cgaagctgga gcaggggcac
1861 ccaggagcag gcctcggagc agcaggcccg ggccgagggc gccctggagc ctgggtgtca
1921 caagcacagt gtggaggttg ccaggagagg gtccttgcca tcccacctcc agctcgcaga
1981 cccgcagggt tcctggcagg agcagcttgc tgccccagaa gagggggaga ccaaaatagc
2041 gctggagaga gagaaggatg acatggaaac caaacttcta catctggaag acgtcgtccg
2101 ggctctggag aaacatgtag atttgagaga gaacgacaga ctggagttcc atagactttc
2161 tgaagaaaac actttgttga aaaacgatct gggaagggtt cggcaagagc ttgaagctgc
2221 agaaagtact cacgatgcac agaggaagga aattgaggtt ttaaagaaag acaaggaaaa
2281 ggcctgctct gagatggagg tgctcaacag acagaatcag aactacaagg atcaattatc
2341 ccagctcaat gtcagggttc ttcaactggg acaggaggct tctacccacc aggcccaaaa
2401 cgaggagcat cgtgtgacca ttcagatgtt aacacagagc ctggaggagg tggttcgcag
2461 tgggcagcag cagagtgacc aaatccaaaa acttagagtt gaacttgaat gcctgaatca
2521 ggaacatcag agcctgcagc tgccatggtc agagctgacc cagacccttg aggaaagtca
2581 agaccaggtg cagggagctc acctgaggct gaggcaggcc caggcccagc acttgcagga
2641 ggtccggctg gtgccccagg accgtgtggc cgagctgcat cgcctgctca gccttcaggg
2701 agagcaggcc aggaggcgcc tggatgcaca gcgggaagaa catgagaaac agctgaaagc
2761 cacagaagag cgggtggaag aggcggagat gattctgaag aatatggaaa tgctcctcca
2821 agagaaagtg gataagctga aggagcagtt tgaaaagaac acgaagtccg acctgctgct
2881 gaaggagctg tacgtggaga acgcccacct ggtgagagca cttcaggcca ccgaggagaa
2941 gcagcgaggc gccgagaaac aaagccgcct cttggaagaa aaagttcgcg ctctcaacaa
3001 actcgtcagt aggattgccc ccgcagccct ctctgtgtgt acagactaca aagaccatga
3061 cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt aactcgagat
3121 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
3181 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
3241 atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac ggcggaactc
3301 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
3361 gtggtgttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat ctagctttat
3421 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
3481 taacaacaac aattgcattc attttatgtt tcaggttcag ggggagatgt gggaggtttt
3541 ttaaagatct ggagaccggt gtggcacgtg cggaccgagc ggccgcagga acccctagtg
3601 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
3661 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc
3721 ctgcaggggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
3781 atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
3841 tggttacgcg cagcgtgacc gctacacttg ccagcgcctt agcgcccgct cctttcgctt
3901 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
3961 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg
4021 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
4081 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
4141 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
4201 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
4261 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
4321 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
4381 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
4441 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
4501 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
4561 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
4621 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
4681 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
4741 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
4801 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
4861 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
4921 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
4981 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
5041 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
5101 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
5161 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
5221 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
5281 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
5341 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
5401 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
5461 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
5521 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
5581 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
5641 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
5701 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
5761 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
5821 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
5881 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
5941 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
6001 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
6061 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
6121 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
6181 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
6241 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
6301 tttgctggcc ttttgctcac atgt
//
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