| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001366 |
| 产品用途: | 3' AAVLINK plasmid for NEXMIF expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
DH5alpha
|
|
插入基因信息 |
|
| 插入基因名称: | NEXMIF |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-NEXMIF-FLAG 6599 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6599)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6599
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3312
/codon_start=1
/label=NEXMIF
/translation="GDSPNPIQ*IWSLPSSLHGK*PHTYNIQSNLSQ*WWQ*LQQGPV*
LHARYPTPI**LLPIMSL**WRDLLSFPAGASQYG*WSAL*L*FNGPTLCQLK*LLLLK
LEVL*KGWR**YH**LPGPLQP*AGDPAKH**DSTTKGVH*PPGYIQLHP*QIPPLFPF
RDVPTGHP*SFPSNYQM*EYEDTRNTKGVPRGCPRTIGQCGKNQVGLQYPFTAGPNGGW
IYFK*SPVSVPYVQ**GFCQPAPKKPLPVNI**SIWSN*YQQQSVKIKKEKFTQQEWGY
EPKLFSEKHQEKIPQR*QQGD*KTTWQKLPPGP*VHKEREIHGCHQWRENANWHWPWGK
PNQHHILHWKDIG*MYPTWWPYGLYEDAKSKGTFWRLGLGEGEQPRLE*YEHGHQHQQP
SG**PTGISGAFLYLVQHCLWYGRCAEIHDGLHRAPLGTHGAPWGSQHILLP*VQ*SKI
KNPQNIGWDTTGV*EKDQQWVPRSH*ESQVNQGCEQKQWENSNR*SWSCKHAWL*RGLS
LYLL**KV**HEHFRQ*RTDTQKVVSSQIQLQGPERREM*GKAHGARTGPQG*VWDSFF
*KTEGFRLQSPKSRNNLLGFTCV*RRDSHFPERH"
misc_feature 1917..1922
/label=SDS
CDS 3319..3384
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3388..3819
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3532..3537
/label=SDS
repeat_region 3862..4002
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3862..3991
/label=AAV2 ITR
rep_origin 4077..4532
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4814..4918
/gene="bla"
/label=AmpR promoter
CDS 4919..5779
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5950..6538
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagatagc cccaacccaa
1501 tccagtgaat ttggagcctc ccaagtagtc tccatggaaa ataacctcac acctacaaca
1561 tacaatccaa tctgtctcaa tagtggtggc agtaattgca acaaggtcct gtatgactcc
1621 atgcaagata cccaactccc atctgatgac tcttaccaat tatgtcactt taataatgga
1681 gagatctgct ttcctttcca gcaggggcca gtcaatatgg atgatggtcg gctctttagc
1741 tttgattcaa tggccccact ctctgtcagc tcaagtaatt attgctcctt aagcttgaag
1801 tcctgtgaaa aggatggcga tgatgatatc actgatgact tcctggccca ttgcagccct
1861 aagctggtga tccagcaaag cattgatgag atagcaccac taaaggagtc cactgacctc
1921 ctggatatat ccaacttcac ccctgacaaa ttccgccact cttccctttc agagatgtcc
1981 ccaccggaca cccctagtct ttcccctcaa attaccagat gtgagagtat gaagacacta
2041 ggaacactaa aggggttcca agagggtgtc ccaggaccat tggacagtgt ggaaaaaatc
2101 aagtgggact gcagtaccct ttcacggcag gtccaaatgg aggatggatt tactttaaat
2161 aatcaccagt ttcagttcca tatgttcaat gatgaggatt ctgtcagcct gctccaaaaa
2221 aacccttgcc tgtcaacatt taatgatcca tctggtcaaa ttagtaccaa caacaaagtg
2281 tcaaaatcaa gaaagaaaag ttcacccagc aagagtgggg ctatgaacca aagctcttct
2341 cagaaaaaca ccaggaaaaa atccctcaaa ggtaacaaca aggggattga aaaaccacct
2401 ggcaaaaact cccgccaggt ccctaagtcc acaaagaaag ggaaatacat ggctgccatc
2461 aatggagaga aaatgcaaat tggcattggc cgtgggggaa gccaaaccaa caccatatcc
2521 tccactggaa agacattggc tgaatgtatc caacatggtg gccctatggc ctctatgaag
2581 atgccaagtc aaaagggact ttctggagat tgggccttgg ggaaggagag cagcccaggc
2641 tggagtgata tgagcatggg caccaacacc aacagccttc tggatgatga ccaacgggaa
2701 tttcaggagc cttcctatat cttgtccaac attgcctctg gtatggcaga tgtgcagaga
2761 ttcatgatgg cctccataga gcccctttgg gaacccatgg agcaccatgg ggatcccaac
2821 atattctact cccctgagtc caatagtcta aaattaaaaa ccctcaaaat attggctggg
2881 acaccacagg agtctaagaa aaagatcaac agtgggtccc aaggagccac taagaatcac
2941 aggtcaatca agggtgtgag caaaagcaat gggaaaacag caataggtga tcctggtcgt
3001 gcaaacatgc ctggttataa cgaggactct cgctctacct tctttgataa aaagtatagt
3061 aacatgagca ctttaggcaa taacggaccg acacacaaaa agttgtatcg tcacaaatcc
3121 agctccaagg ccctgagaga cgagaaatgt aagggaaagc acatggagcg agaacaggtc
3181 cacaaggatg agtctgggac agcttctttt gaaaaactga gggattccga ctacaatctc
3241 ctaaaagcag aaacaacctt ttgggtttta cctgtgtttg aagaagagac tcgcattttc
3301 cagaaagaca tttgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc
3361 gactacaagg atgacgatga caagtaactc gagataatca acctctggat tacaaaattt
3421 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
3481 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
3541 ataaatcctg gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
3601 ggacaggggc tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg
3661 atgctattgc tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct
3721 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
3781 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc
3841 acgtgcggac cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc
3901 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
3961 gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt
4021 ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc
4081 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
4141 acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
4201 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
4261 tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc
4321 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
4381 cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg
4441 gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc
4501 gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc
4561 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
4621 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
4681 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
4741 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
4801 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
4861 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
4921 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
4981 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
5041 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
5101 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
5161 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
5221 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
5281 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
5341 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
5401 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
5461 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
5521 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
5581 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
5641 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
5701 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
5761 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
5821 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
5881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
5941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
6001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
6061 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
6121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
6181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
6241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
6301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
6361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
6421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
6481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
6541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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