| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001363 |
| 产品用途: | 5' AAVLINK plasmid for MBD5 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MBD5 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG 6289 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6289)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6289
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3002
/codon_start=1
/label=MBD5
/translation="GYQNLQAFQGQSTIPCPANNNPMACLFQNFQVRMQEDAALLNKRI
STQPGLTALPENPNTTLPPFQDTPCELQPRIDPSLGQQVKDGLVVGGPGDASVDAIYKA
VVDAASKGMQVVITTAVNSTTQISPIPALSAMSAFTASIGDPLNLSSVVSAVIHGRNMG
GVDHDGRLRNSRGARLPKNLDHGKNVNEGDGFEYFKSASCHTSKKQWDGEQSPRGERNR
WKYEEFLDHPGHIHSSPCHERPNNVSTLPFLPGEQHPILLPPRNCPGDKILEENFRYNN
YKRTMMSFKERLENTVERCAHINGNRPRQSRGFGELLSTAKQDLVLEEQSPSSSNSLEN
SLVKDYIHYNGDFNAKSVNGCVPSPSDAKSISSEDDLRNPDSPSSNELIHYRPRTFNVG
DLVWGQIKGLTSWPGKLVREDDVHNSCQQSPEEGKVEPEKLKTLTEGLEAYSRVRKRNR
KSGKLNNHLEAAIHEAMSELDKMSGTVHQIPQGDRQMRPPKPKRRKISR"
misc_feature 2985..2990
/label=SDS
CDS 3009..3074
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3078..3509
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3222..3227
/label=SDS
repeat_region 3552..3692
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3552..3681
/label=AAV2 ITR
rep_origin 3767..4222
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4504..4608
/gene="bla"
/label=AmpR promoter
CDS 4609..5469
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5640..6228
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggtaccag aatctccagg
1501 cgttccaagg acagtccaca attccttgcc cagctaacaa taaccccatg gcttgtctgt
1561 ttcagaactt tcaggtgaga atgcaggaag atgcagctct cctaaacaaa agaataagca
1621 ctcagcctgg gctcacagca cttcctgaga atccaaacac tacacttcca ccttttcaag
1681 atacaccttg tgagttgcaa ccgaggattg acccatctct tggtcaacag gtgaaggatg
1741 gcctcgttgt gggtggccca ggtgatgctt ccgtagatgc catttacaaa gcagttgtcg
1801 atgcagccag caaaggaatg caggttgtca tcaccactgc agtcaacagt acaactcaga
1861 tcagccccat tccagctctg agtgccatga gtgccttcac tgcctcaatt ggtgacccat
1921 taaatctctc cagtgttgtc agtgcggtca ttcatggacg gaacatggga ggtgttgatc
1981 atgatggtag gctgaggaat tcaagagggg ctcggctgcc caagaatcta gaccatggga
2041 aaaatgtgaa cgaaggagat gggtttgaat atttcaagtc agcaagttgc cacacatcca
2101 aaaaacagtg ggacggggag caaagcccca gaggggagcg aaacaggtgg aagtacgagg
2161 aatttttaga tcatccaggc catatccaca gtagtccttg tcatgaaagg cccaacaatg
2221 tctctacact gccatttctg cctggggaac agcacccaat actgttacca ccaagaaact
2281 gtccagggga taaaattcta gaggaaaatt tcaggtataa taactacaaa agaactatga
2341 tgagttttaa ggagagacta gagaacactg tggaaagatg tgcacacata aatgggaata
2401 gacctcgaca gagtcgggga tttggagagc tgctaagcac tgcaaagcaa gacctggtcc
2461 tagaggagca gtctccaagt tcctcaaata gtttggaaaa ttctctggtc aaagactaca
2521 tccattacaa tggagacttt aatgccaaaa gcgttaatgg gtgtgtgcct agcccttcag
2581 atgctaaaag cattagtagt gaagatgacc taaggaaccc agactccccc tcttcaaatg
2641 aattgataca ttatagacca aggacgttca atgttggcga cttggtctgg ggccaaatca
2701 aaggactgac ttcctggcct ggaaaattag taagagaaga cgacgttcac aattcatgtc
2761 aacaaagccc cgaggaaggg aaggtggagc ccgagaagtt gaagacacta acagaaggtt
2821 tggaagccta cagccgtgtc cggaaaagga acagaaaaag tggaaagcta aataaccatt
2881 tagaagctgc tattcatgag gccatgagtg aactggacaa aatgtctggg actgtacacc
2941 aaatcccaca gggtgacaga caaatgagac cccccaaacc caagaggagg aagatctcca
3001 gatgtacaga ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg
3061 atgacgatga caagtaactc gagataatca acctctggat tacaaaattt gtgaaagatt
3121 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
3181 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
3241 gttagttctt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
3301 tcggctgttg ggcactgaca attccgtggt gtttatttgt gaaatttgtg atgctattgc
3361 tttatttgta accatctagc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
3421 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
3481 ttcaggggga gatgtgggag gttttttaaa gatctggaga ccggtgtggc acgtgcggac
3541 cgagcggccg caggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
3601 gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc
3661 agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg atgcggtatt ttctccttac
3721 gcatctgtgc ggtatttcac accgcatacg tcaaagcaac catagtacgc gccctgtagc
3781 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
3841 gccttagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
3901 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
3961 ctcgacccca aaaaacttga tttgggtgat ggttcacgta gtgggccatc gccctgatag
4021 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
4081 actggaacaa cactcaactc tatctcgggc tattcttttg atttataagg gattttgccg
4141 atttcggtct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
4201 aaaatattaa cgtttacaat tttatggtgc actctcagta caatctgctc tgatgccgca
4261 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
4321 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
4381 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta
4441 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
4501 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
4561 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
4621 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
4681 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
4741 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
4801 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
4861 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
4921 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
4981 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
5041 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
5101 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
5161 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
5221 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
5281 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
5341 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
5401 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
5461 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
5521 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
5581 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
5641 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
5701 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
5761 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
5821 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
5881 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
5941 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
6001 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
6061 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
6121 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
6181 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
6241 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgt
//
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pAAVLINKv1-pCALM1-Cre-3'-MBD5-FLAG质粒载体应用举例
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