| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001426 |
| 产品用途: | 3' AAVLINK plasmid for KIAA0232 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KIAA0232 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KIAA0232-FLAG 6476 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6476)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 7, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
FEATURES Location/Qualifiers
source 1..6476
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3199
/codon_start=1
/label=KIAA0232
/translation="GLLKTFGQRWQTQILWLQ*K*KELMLSCFRQM*ITTAAV*MLKLN
WRPFRSLIRLCGGQSTICGRDRKRAWRKEHLLLVSYQTWMVVIIQHPLNPGM*PKIKKT
HSFLEEFMENSKPSIVMGSGQSYHLVTQKEVCYSVQLLML*R*LVQMSL*PQETVLLLG
TGSYGNPSCHLNRMISRRVGKMG*IRDFLLSSMKTY*ELVATFKSKILDLNTHFLPLT*
AIHFHKFFM*NAHLNLKGLHLSAPVLNRNQSSVLIQTVKCFTPGYVVLTEHNTGLFGSL
LGLTFAQFLHPNCPQEEEASQNLNLRKMKQIFPFLLKLIYLKIRRQISNLWKKMQRKKA
ITMENQSLSLENSFPG*KNLGWKRVLRHHWIPRRNQLGFFQ*ESKISVWNVA*MNPWK*
I*KAQKQIVK*WHNARKKLIIFVVAKQVVSFLLMKIIQFLRDSWKSSLY*TLIYKE*IE
VKKNRPGGKKPCTLLFFLHQSVKNVTQMPRERVV*KNIQMLKRHPVMKSAC*ILIGCLL
FMKQDVQEREILEQSQMASAERCAPAPAPPRKRQAQKAEASGWALVKRSSFLELI"
misc_feature 2368..2373
/label=SDS
misc_feature 2371..2376
/label=SDS
misc_feature 2598..2603
/label=SDS
CDS 3206..3271
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3287..3718
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3431..3436
/label=SDS
repeat_region 3761..3879
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3761..3868
/label=AAV2 ITR
rep_origin 3954..4409
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4691..4795
/gene="bla"
/label=AmpR promoter
CDS 4796..5656
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5827..6415
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggttatta aagacatttg
1501 gacaaagatg gcagacacaa attctgtggc tacagtagaa atagaaagaa ctgatgctga
1561 gttgttttcg gcagatgtaa ataactactg ctgctgtcta gatgctgaag ctgaactgga
1621 gacccttcag gagcctgata aggctgtgcg gaggtcagag taccatctgt gggagggaca
1681 gaaagagagc ctggagaaaa gagcatttgc ttctagtgag ctatcaaacg tggatggtgg
1741 tgattataca acaccctcta aaccctggga tgtagcccaa gataaagaaa acacattcat
1801 tcttggagga gtttatggag aactcaaaac cttcaatagt gatggggagt gggcagtcgt
1861 accacctagt cacacaaaag gaagtctgtt acagtgtgca gcttctgatg ttgtgacgat
1921 agctggtaca gatgtcttta tgaccccagg aaacagtttt gctcctgggc acaggcagtt
1981 atggaaaccc ttcgtgtcat ttgaacagaa tgatcagccg aagagtgggg aaaatgggtt
2041 aaataaggga ttttctttta tcttccatga agacttacta ggagcttgtg gcaactttca
2101 agtcgaagat cctggacttg aatactcatt ttcttccttt gacttaagca atccattttc
2161 acaagttctt catgtagaat gctcatttga acctgaaggg attgcatctt tcagccccag
2221 ttttaaaccg aaatcaatcc tctgttctga ttcagacagt gaagtgtttc accccaggat
2281 atgtggtgtt gacagaacac aatacagggc tattcggatc tctcctcgga ctcactttcg
2341 cccaatttct gcatccgaac tgtccccagg aggaggaagc gagtcagaat ttgaatctga
2401 gaaagatgaa gcaaatattc ccattccttc tcaagttgat atatttgaag atccgcaggc
2461 agatctcaaa cctttggaag aagatgcaga gaaagaaggc cattactatg gaaaatcaga
2521 gcttgagtct ggaaaattcc ttcccaggtt aaaaaaatct gggatggaaa agagtgctca
2581 gacatcactg gattcccagg aggaatcaac tgggattctt tcagtaggaa agcaaaatca
2641 gtgtttggaa tgtagcatga atgaatccct ggaaatagat ttagaaagct cagaagcaaa
2701 ttgtaaaata atggcacaat gcgaggaaga aattaataat ttttgtggtt gcaaagcagg
2761 ttgtcagttt cctgcttatg aagataatcc agtttcttcg ggacagctgg aagagttccc
2821 tgtattgaac actgatatac aaggaatgaa tagaagtcaa gaaaaacaga cctggtggga
2881 aaaagccttg tactctcctc tttttcctgc atcagagtgt gaagaatgtt acacaaatgc
2941 caagggagag agtggtttag aagaatatcc agatgctaaa gagacaccca gtaatgaaga
3001 gcgcctgtta gattttaata gggtgtcttc tgtttatgaa gcaagatgta caggagagag
3061 agattctgga gcaaagtcag atggcttccg cggaaagatg tgctccagcg ccagctccac
3121 ctcggaagag acaggctcag aaggcggagg cgagtgggtg ggccctagtg aagaggagct
3181 cttttctcga actcatctct gtacagacta caaagaccat gacggtgatt ataaagatca
3241 tgacatcgac tacaaggatg acgatgacaa gtaagaattc tgatgactcg agataatcaa
3301 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
3361 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
3421 ttcattttct cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc
3481 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
3541 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga
3601 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
3661 caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaaag
3721 atctggagac cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag
3781 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
3841 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
3901 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
3961 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
4021 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
4081 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
4141 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
4201 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
4261 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
4321 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
4381 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
4441 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
4501 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
4561 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
4621 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
4681 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
4741 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
4801 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
4861 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
4921 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
4981 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
5041 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
5101 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
5161 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
5221 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
5281 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
5341 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
5401 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
5461 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
5521 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
5581 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
5641 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
5701 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
5761 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
5821 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
5881 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
5941 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
6001 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
6061 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
6121 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
6181 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
6241 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
6301 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
6361 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
6421 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//
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