| 质粒名称: | pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001390 |
| 产品用途: | 3' AAVLINK plasmid for KDM6B expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒表达 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | KDM6B |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
买家导航
pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-KDM6B-FLAG 6704 bp DNA circular SYN 11-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6704)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported May 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6704
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3405
/codon_start=1
/label=KDM6B
/translation="GKGQGQGPQRKEPPGAGEPGPAERGDPGS*EVPARSWRGLQGQAT
HSSSPSISSCTFCPAHTPVSLCPWKEGSGGSPRATGCQPGRHAEAALT**GAPQGAEDP
AHQGREW*QGDLYRL*GGRAAAAHGRPHHQPLCC*RRARQQECQGEREVSRVLPFPCPV
CETEDQH*GEAAPGKTQPPYTQHLSGEQTGCLLTCPAAVLYRPSKSHHSDPGPGGLPAA
QLGPLLHQDPGGSEWRTHRGSSHPGAAALR*ELGSDRHSADLAL*ELPFPHHHCQVRTV
PGLILPGVSAGGEGE*G*GVRGARQHHWNPS*QRTRPEEPSHHQVWHQHRLV*C*AVEA
PAAGAAKAARLHAGNIHGQHAEPRGPHHPGHEHGAAVHEGARQPNARPPGE*QLLLRQH
QHWPRRLRVVRGARALLGDHQRFL*SARRGLLDGFLVANPG*SLCIQYSCVPLRAATRR
PRVD*CGDCALGAGHRLVQQHCLERGAPHRLSVPAGPGTIRVE*GEERQIHRAHDSRVM
ERGSHGQNQRPRLVQDDQVLPAAVHEALPGATREPGAGREENRLPGPCQGRASLLLQRV
RCGGV*HPVRDK*EWQPQHVPGTLRGLCPAPQRRPAGRGGAGAVPH*GAGSGLRRLHAG
PSQHVA"
misc_feature 2354..2359
/label=SDS
misc_feature 2413..2418
/label=SDS
CDS 3412..3477
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3493..3924
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3637..3642
/label=SDS
repeat_region 3967..4107
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3967..4096
/label=AAV2 ITR
rep_origin 4182..4637
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4919..5023
/gene="bla"
/label=AmpR promoter
CDS 5024..5884
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6055..6643
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcaaaggc caaggccaag
1501 gtccccaaag aaaagagccg ccgggtgctg gggaacctgg acctgcagag cgaggagatc
1561 cagggtcgtg agaagtcccg gcccgatctt ggcggggcct ccaaggccaa gccacccaca
1621 gctccagccc ctccatcagc tcctgcacct tctgcccagc ccacaccccc gtcagcctct
1681 gtccctggaa agaaggctcg ggaggaagcc ccagggccac cgggtgtcag ccgggccgac
1741 atgctgaagc tgcgctcact tagtgagggg ccccccaagg agctgaagat ccggctcatc
1801 aaggtagaga gtggtgacaa ggagaccttt atcgcctctg aggtggaaga gcggcggctg
1861 cgcatggcag acctcaccat cagccactgt gctgctgacg tcgtgcgcgc cagcaggaat
1921 gccaaggtga aagggaagtt tcgagagtcc tacctttccc ctgcccagtc tgtgaaaccg
1981 aagatcaaca ctgaggagaa gctgccccgg gaaaaactca acccccctac acccagcatc
2041 tatctggaga gcaaacggga tgccttctca cctgtcctgc tgcagttctg tacagaccct
2101 cgaaatccca tcacagtgat ccggggcctg gcgggctccc tgcggctcaa cttgggcctc
2161 ttctccacca agaccctggt ggaagcgagt ggcgaacaca ccgtggaagt tcgcacccag
2221 gtgcagcagc cctcagatga gaactgggat ctgacaggca ctcggcagat ctggccttgt
2281 gagagctccc gttcccacac caccattgcc aagtacgcac agtaccaggc ctcatccttc
2341 caggagtctc tgcaggagga gaaggagagt gaggatgagg agtcagagga gccagacagc
2401 accactggaa cccctcctag cagcgcacca gacccgaaga accatcacat catcaagttt
2461 ggcaccaaca tcgacttgtc tgatgctaag cggtggaagc cccagctgca ggagctgcta
2521 aagctgcccg ccttcatgcg ggtaacatcc acgggcaaca tgctgagcca cgtgggccac
2581 accatcctgg gcatgaacac ggtgcagctg tacatgaagg tgcccggcag ccgaacgcca
2641 ggccaccagg agaataacaa cttctgctcc gtcaacatca acattggccc aggcgactgc
2701 gagtggttcg cggtgcacga gcactactgg gagaccatca gcgctttctg tgatcggcac
2761 ggcgtggact acttgacggg ttcctggtgg ccaatcctgg atgatctcta tgcatccaat
2821 attcctgtgt accgcttcgt gcagcgaccc ggagacctcg tgtggattaa tgcggggact
2881 gtgcactggg tgcaggccac cggctggtgc aacaacattg cctggaacgt ggggcccctc
2941 accgcctatc agtaccagct ggccctggaa cgatacgagt ggaatgaggt gaagaacgtc
3001 aaatccatcg tgcccatgat tcacgtgtca tggaacgtgg ctcgcacggt caaaatcagc
3061 gaccccgact tgttcaagat gatcaagttc tgcctgctgc agtccatgaa gcactgccag
3121 gtgcaacgcg agagcctggt gcgggcaggg aagaaaatcg cttaccaggg ccgtgtcaag
3181 gacgagccag cctactactg caacgagtgc gatgtggagg tgtttaacat cctgttcgtg
3241 acaagtgaga atggcagccg caacacgtac ctggtacact gcgagggctg tgcccggcgc
3301 cgcagcgcag gcctgcaggg cgtggtggtg ctggagcagt accgcactga ggagctggct
3361 caggcctacg acgccttcac gctggcccca gccagcacgt cgcgatgtac agactacaaa
3421 gaccatgacg gtgattataa agatcatgac atcgactaca aggatgacga tgacaagtaa
3481 gaattctgat gactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
3541 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
3601 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
3661 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
3721 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
3781 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
3841 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
3901 ggggagatgt gggaggtttt ttaaagatct ggagaccggt gtggcacgtg cggaccgagc
3961 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
4021 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
4081 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
4141 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
4201 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgcctt
4261 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
4321 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
4381 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
4441 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
4501 aacaacactc aactctatct cgggctattc ttttgattta taagggattt tgccgatttc
4561 ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
4621 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
4681 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
4741 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
4801 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
4861 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
4921 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
4981 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
5041 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
5101 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
5161 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
5221 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
5281 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
5341 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
5401 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
5461 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
5521 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
5581 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
5641 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
5701 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
5761 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
5821 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
5881 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
5941 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
6001 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
6061 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
6121 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
6181 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
6241 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
6301 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
6361 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
6421 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
6481 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
6541 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
6601 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
6661 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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